1, 10–Phenanthroline potentiates cytotoxicity of hydroxyurea in human chronic myeloid leukemia cells

Narayana S. Kamath, K. Satyamoorthy, Manik P. Chitnis, Suresh H. Advani

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    The effect of the divalent hydrophobic metal chelator 1, 10-phenanthroline was evaluated alone and in combination with the antineoplastic agent hydroxyurea on human chronic myeloid leukemia cells. The compound at concentrations of 10 and 5 μg/ml significantly (p <0.001) potentiates DNA biosynthesis inhibiting the activity of hydroxyurea in vitro. Synergistic inhibition was obtained when both 1, 10-phenanthroline and hydroxyurea was used in combination at relatively nontoxic concentrations. Cytotoxicity due to the combination was found to be partially reversible and was marginally reversible in the presence of Fe+2 and Zn. The results strongly suggest the utility of the iron-chelating agent along with hydroxyurea for further evaluation to achieve a better therapeutic result in the clinic.

    Original languageEnglish
    Pages (from-to)193-197
    Number of pages5
    JournalOncology (Switzerland)
    Volume46
    Issue number3
    DOIs
    Publication statusPublished - 1989

    Fingerprint

    Hydroxyurea
    Myeloid Cells
    Leukemia, Myelogenous, Chronic, BCR-ABL Positive
    Iron Chelating Agents
    Chelating Agents
    Antineoplastic Agents
    Metals
    DNA
    1,10-phenanthroline
    Therapeutics

    All Science Journal Classification (ASJC) codes

    • Oncology
    • Cancer Research

    Cite this

    Kamath, Narayana S. ; Satyamoorthy, K. ; Chitnis, Manik P. ; Advani, Suresh H. / 1, 10–Phenanthroline potentiates cytotoxicity of hydroxyurea in human chronic myeloid leukemia cells. In: Oncology (Switzerland). 1989 ; Vol. 46, No. 3. pp. 193-197.
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    1, 10–Phenanthroline potentiates cytotoxicity of hydroxyurea in human chronic myeloid leukemia cells. / Kamath, Narayana S.; Satyamoorthy, K.; Chitnis, Manik P.; Advani, Suresh H.

    In: Oncology (Switzerland), Vol. 46, No. 3, 1989, p. 193-197.

    Research output: Contribution to journalArticle

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    AU - Kamath, Narayana S.

    AU - Satyamoorthy, K.

    AU - Chitnis, Manik P.

    AU - Advani, Suresh H.

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    AB - The effect of the divalent hydrophobic metal chelator 1, 10-phenanthroline was evaluated alone and in combination with the antineoplastic agent hydroxyurea on human chronic myeloid leukemia cells. The compound at concentrations of 10 and 5 μg/ml significantly (p <0.001) potentiates DNA biosynthesis inhibiting the activity of hydroxyurea in vitro. Synergistic inhibition was obtained when both 1, 10-phenanthroline and hydroxyurea was used in combination at relatively nontoxic concentrations. Cytotoxicity due to the combination was found to be partially reversible and was marginally reversible in the presence of Fe+2 and Zn. The results strongly suggest the utility of the iron-chelating agent along with hydroxyurea for further evaluation to achieve a better therapeutic result in the clinic.

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