1-Triacontanol cerotate; isolated from Marsilea quadrifolia Linn. ameliorates reactive oxidative damage in the frontal cortex and hippocampus of chronic epileptic rats

Adhikari Snehunsu, Chitrini Ghosal, Mamta Kandwal, Pramod K. Yadav, B. Satheesha Nayak, K. Raghavendra Rao, Shobha U. Kamath, Pabitra Sahoo, K. K. Srinivasan, Sareesh Naduvil Narayanan, Shiva Kumar, Alex Joseph

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Ethnopharmacological relevance: Marsilea quadrifolia Linn. (MQ) has been used for insomnia and epileptic disorders in traditional Indian medicine. The present study is to isolate the active component responsible for antiepileptic property of MQ by evaluating its ability to minimize the reactive oxidative damage in brain due to chronic epilepsy in rat. Materials and methods: 1-Triacontanol cerotate (1TAC) was isolated after chromatography on a silica gel from dried petroleum ether fraction of methanolic extract of MQ. Acute oral toxicity studies of 1TAC were carried out and efficacy of 1TAC on malondialdehyde (MDA) and reduced glutathione (GSH) production in different brain areas of chronic pentylenetetrazole (PTZ) induced epileptic rats were evaluated. Results: Our results showed that PTZ-kindled chronic epileptic rats had an increase MDA and decreased GSH concentration in the frontal cortex as well as hippocampus, compared to the normal control. MDA and GSH concentrations in those brain areas were normalized after treatment with sodium valproate (SV) in 200 mg kg-1 bw; as well as 1TAC in 40 and 80 mg kg-1 bw doses. Conclusion: Production of reactive oxygen species (ROS) is known to worsen epileptogenesis. The isolated component 1TAC which reduced the reactive oxidative damage in hippocampus and frontal cortex of PTZ kindled rats could be responsible for antiepileptic property of MQ. Its action is found to be dose dependent, with 80 mg kg-1 bw showing even better efficacy than 200 mg kg-1 bw of SV.

Original languageEnglish
Pages (from-to)80-84
Number of pages5
JournalJournal of Ethnopharmacology
Volume172
DOIs
Publication statusPublished - 06-07-2015

Fingerprint

Marsileaceae
Frontal Lobe
Hippocampus
Pentylenetetrazole
Malondialdehyde
Valproic Acid
Anticonvulsants
Brain
Aptitude
Silica Gel
Sleep Initiation and Maintenance Disorders
Traditional Medicine
Glutathione
Chromatography
Epilepsy
Reactive Oxygen Species
1-triacontanol
hexacosanoic acid

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

@article{1f2f37dede4141fa9d61dd6db96610e9,
title = "1-Triacontanol cerotate; isolated from Marsilea quadrifolia Linn. ameliorates reactive oxidative damage in the frontal cortex and hippocampus of chronic epileptic rats",
abstract = "Ethnopharmacological relevance: Marsilea quadrifolia Linn. (MQ) has been used for insomnia and epileptic disorders in traditional Indian medicine. The present study is to isolate the active component responsible for antiepileptic property of MQ by evaluating its ability to minimize the reactive oxidative damage in brain due to chronic epilepsy in rat. Materials and methods: 1-Triacontanol cerotate (1TAC) was isolated after chromatography on a silica gel from dried petroleum ether fraction of methanolic extract of MQ. Acute oral toxicity studies of 1TAC were carried out and efficacy of 1TAC on malondialdehyde (MDA) and reduced glutathione (GSH) production in different brain areas of chronic pentylenetetrazole (PTZ) induced epileptic rats were evaluated. Results: Our results showed that PTZ-kindled chronic epileptic rats had an increase MDA and decreased GSH concentration in the frontal cortex as well as hippocampus, compared to the normal control. MDA and GSH concentrations in those brain areas were normalized after treatment with sodium valproate (SV) in 200 mg kg-1 bw; as well as 1TAC in 40 and 80 mg kg-1 bw doses. Conclusion: Production of reactive oxygen species (ROS) is known to worsen epileptogenesis. The isolated component 1TAC which reduced the reactive oxidative damage in hippocampus and frontal cortex of PTZ kindled rats could be responsible for antiepileptic property of MQ. Its action is found to be dose dependent, with 80 mg kg-1 bw showing even better efficacy than 200 mg kg-1 bw of SV.",
author = "Adhikari Snehunsu and Chitrini Ghosal and Mamta Kandwal and Yadav, {Pramod K.} and Nayak, {B. Satheesha} and {Raghavendra Rao}, K. and Kamath, {Shobha U.} and Pabitra Sahoo and Srinivasan, {K. K.} and {Naduvil Narayanan}, Sareesh and Shiva Kumar and Alex Joseph",
year = "2015",
month = "7",
day = "6",
doi = "10.1016/j.jep.2015.06.020",
language = "English",
volume = "172",
pages = "80--84",
journal = "Journal of Ethnopharmacology",
issn = "0378-8741",
publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - 1-Triacontanol cerotate; isolated from Marsilea quadrifolia Linn. ameliorates reactive oxidative damage in the frontal cortex and hippocampus of chronic epileptic rats

AU - Snehunsu, Adhikari

AU - Ghosal, Chitrini

AU - Kandwal, Mamta

AU - Yadav, Pramod K.

AU - Nayak, B. Satheesha

AU - Raghavendra Rao, K.

AU - Kamath, Shobha U.

AU - Sahoo, Pabitra

AU - Srinivasan, K. K.

AU - Naduvil Narayanan, Sareesh

AU - Kumar, Shiva

AU - Joseph, Alex

PY - 2015/7/6

Y1 - 2015/7/6

N2 - Ethnopharmacological relevance: Marsilea quadrifolia Linn. (MQ) has been used for insomnia and epileptic disorders in traditional Indian medicine. The present study is to isolate the active component responsible for antiepileptic property of MQ by evaluating its ability to minimize the reactive oxidative damage in brain due to chronic epilepsy in rat. Materials and methods: 1-Triacontanol cerotate (1TAC) was isolated after chromatography on a silica gel from dried petroleum ether fraction of methanolic extract of MQ. Acute oral toxicity studies of 1TAC were carried out and efficacy of 1TAC on malondialdehyde (MDA) and reduced glutathione (GSH) production in different brain areas of chronic pentylenetetrazole (PTZ) induced epileptic rats were evaluated. Results: Our results showed that PTZ-kindled chronic epileptic rats had an increase MDA and decreased GSH concentration in the frontal cortex as well as hippocampus, compared to the normal control. MDA and GSH concentrations in those brain areas were normalized after treatment with sodium valproate (SV) in 200 mg kg-1 bw; as well as 1TAC in 40 and 80 mg kg-1 bw doses. Conclusion: Production of reactive oxygen species (ROS) is known to worsen epileptogenesis. The isolated component 1TAC which reduced the reactive oxidative damage in hippocampus and frontal cortex of PTZ kindled rats could be responsible for antiepileptic property of MQ. Its action is found to be dose dependent, with 80 mg kg-1 bw showing even better efficacy than 200 mg kg-1 bw of SV.

AB - Ethnopharmacological relevance: Marsilea quadrifolia Linn. (MQ) has been used for insomnia and epileptic disorders in traditional Indian medicine. The present study is to isolate the active component responsible for antiepileptic property of MQ by evaluating its ability to minimize the reactive oxidative damage in brain due to chronic epilepsy in rat. Materials and methods: 1-Triacontanol cerotate (1TAC) was isolated after chromatography on a silica gel from dried petroleum ether fraction of methanolic extract of MQ. Acute oral toxicity studies of 1TAC were carried out and efficacy of 1TAC on malondialdehyde (MDA) and reduced glutathione (GSH) production in different brain areas of chronic pentylenetetrazole (PTZ) induced epileptic rats were evaluated. Results: Our results showed that PTZ-kindled chronic epileptic rats had an increase MDA and decreased GSH concentration in the frontal cortex as well as hippocampus, compared to the normal control. MDA and GSH concentrations in those brain areas were normalized after treatment with sodium valproate (SV) in 200 mg kg-1 bw; as well as 1TAC in 40 and 80 mg kg-1 bw doses. Conclusion: Production of reactive oxygen species (ROS) is known to worsen epileptogenesis. The isolated component 1TAC which reduced the reactive oxidative damage in hippocampus and frontal cortex of PTZ kindled rats could be responsible for antiepileptic property of MQ. Its action is found to be dose dependent, with 80 mg kg-1 bw showing even better efficacy than 200 mg kg-1 bw of SV.

UR - http://www.scopus.com/inward/record.url?scp=84935497079&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84935497079&partnerID=8YFLogxK

U2 - 10.1016/j.jep.2015.06.020

DO - 10.1016/j.jep.2015.06.020

M3 - Article

C2 - 26117530

AN - SCOPUS:84935497079

VL - 172

SP - 80

EP - 84

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

SN - 0378-8741

ER -