3D/4D multiscale imaging in acute lymphoblastic leukemia cells: Visualizing dynamics of cell death

Sreelatha Sarangapani, Rosmin Elsa Mohan, Ajeetkumar Patil, Matthew J. Lang, Anand Asundi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Quantitative phase detection is a new methodology that provides quantitative information on cellular morphology to monitor the cell status, drug response and toxicity. In this paper the morphological changes in acute leukemia cells treated with chitosan were detected using d'Bioimager a robust imaging system. Quantitative phase image of the cells was obtained with numerical analysis. Results show that the average area and optical volume of the chitosan treated cells is significantly reduced when compared with the control cells, which reveals the effect of chitosan on the cancer cells. From the results it can be attributed that d'Bioimager can be used as a non-invasive imaging alternative to measure the morphological changes of the living cells in real time.

Original languageEnglish
Title of host publicationFifth International Conference on Optical and Photonics Engineering
PublisherSPIE
Volume10449
ISBN (Electronic)9781510613720
DOIs
Publication statusPublished - 01-01-2017
Event5th International Conference on Optical and Photonics Engineering - Singapore, Singapore
Duration: 04-04-201707-04-2017

Conference

Conference5th International Conference on Optical and Photonics Engineering
CountrySingapore
CitySingapore
Period04-04-1707-04-17

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Sarangapani, S., Mohan, R. E., Patil, A., Lang, M. J., & Asundi, A. (2017). 3D/4D multiscale imaging in acute lymphoblastic leukemia cells: Visualizing dynamics of cell death. In Fifth International Conference on Optical and Photonics Engineering (Vol. 10449). [1044936] SPIE. https://doi.org/10.1117/12.2270979