5-Amino salicylic acid inhibits nitrite-induced methemoglobin formation

R.B. Miniyar, T.G. Mahesh, M.K. Unnikrishnan

Research output: Contribution to journalArticle

Abstract

5 Amino salicylic acid (5-ASA) and several structurally-related analogs were tested for inhibitory role on nitrite-induced oxidation of hemoglobin in human blood-hemolysates. Results indicate a strong inhibitory role for 5-ASA in a concentration range of 0.2 to 0.8 mM. Although the inhibitory activity of 5-ASA was higher than other structural alalogs tested, the activity of curcumin was much higher at equimolar levels. PABA also showed some activity at similar concentrations although it was lower than that of 5-ASA. Benzoic acid showed marginal activity at lower concentrations. On the other hand, acetyl salicylic acid and salicylic acid increased the methemoglobin levels in hemolysate. The pro-oxidant activity was higher at higher concentrations for these compounds. Higher level of activity of 5-ASA is consistent with previous findings. As acetyl salicylic acid and 5-ASA are currently being prescribed, it is worth investigating their in vivo activity in order to establish novel and hitherto unexplored clinical applications.
Original languageEnglish
Pages (from-to)466-468
Number of pages3
JournalIndian Journal of Pharmaceutical Sciences
Volume62
Issue number6
Publication statusPublished - 2000

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Methemoglobin
Salicylic Acid
Nitrites
4-Aminobenzoic Acid
Curcumin
Benzoic Acid
Reactive Oxygen Species
Hemoglobins

Cite this

Miniyar, R. B., Mahesh, T. G., & Unnikrishnan, M. K. (2000). 5-Amino salicylic acid inhibits nitrite-induced methemoglobin formation. Indian Journal of Pharmaceutical Sciences, 62(6), 466-468.
Miniyar, R.B. ; Mahesh, T.G. ; Unnikrishnan, M.K. / 5-Amino salicylic acid inhibits nitrite-induced methemoglobin formation. In: Indian Journal of Pharmaceutical Sciences. 2000 ; Vol. 62, No. 6. pp. 466-468.
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abstract = "5 Amino salicylic acid (5-ASA) and several structurally-related analogs were tested for inhibitory role on nitrite-induced oxidation of hemoglobin in human blood-hemolysates. Results indicate a strong inhibitory role for 5-ASA in a concentration range of 0.2 to 0.8 mM. Although the inhibitory activity of 5-ASA was higher than other structural alalogs tested, the activity of curcumin was much higher at equimolar levels. PABA also showed some activity at similar concentrations although it was lower than that of 5-ASA. Benzoic acid showed marginal activity at lower concentrations. On the other hand, acetyl salicylic acid and salicylic acid increased the methemoglobin levels in hemolysate. The pro-oxidant activity was higher at higher concentrations for these compounds. Higher level of activity of 5-ASA is consistent with previous findings. As acetyl salicylic acid and 5-ASA are currently being prescribed, it is worth investigating their in vivo activity in order to establish novel and hitherto unexplored clinical applications.",
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note = "Export Date: 10 November 2017 CODEN: IJSID Correspondence Address: Unnikrishnan, M.K.; College of Pharmaceutical Sciences, Manipal-576 119, India References: Miles, A.M., Grisham, M.B., (1994) Methods in Enzymology, 234, p. 555; Doyle, M.P., Pickering, R.A., Dykstra, R.L., Nelson, C.L., Boyer, R.F., (1982) Biochem. Biophys. Res. Commun., 105, p. 127; Smith, R.C., Nunn, V., (1984) Arch Biochem Biphys, 232, p. 348; Unnikrishnan, M.K., Rao, M.N.A., (1995) Pharmazie., 50, p. 490; Unnikrishnan, M.K., Rao, M.N.A., (1995) Mol. Cell. Biochem., 146, p. 35",
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Miniyar, RB, Mahesh, TG & Unnikrishnan, MK 2000, '5-Amino salicylic acid inhibits nitrite-induced methemoglobin formation', Indian Journal of Pharmaceutical Sciences, vol. 62, no. 6, pp. 466-468.

5-Amino salicylic acid inhibits nitrite-induced methemoglobin formation. / Miniyar, R.B.; Mahesh, T.G.; Unnikrishnan, M.K.

In: Indian Journal of Pharmaceutical Sciences, Vol. 62, No. 6, 2000, p. 466-468.

Research output: Contribution to journalArticle

TY - JOUR

T1 - 5-Amino salicylic acid inhibits nitrite-induced methemoglobin formation

AU - Miniyar, R.B.

AU - Mahesh, T.G.

AU - Unnikrishnan, M.K.

N1 - Export Date: 10 November 2017 CODEN: IJSID Correspondence Address: Unnikrishnan, M.K.; College of Pharmaceutical Sciences, Manipal-576 119, India References: Miles, A.M., Grisham, M.B., (1994) Methods in Enzymology, 234, p. 555; Doyle, M.P., Pickering, R.A., Dykstra, R.L., Nelson, C.L., Boyer, R.F., (1982) Biochem. Biophys. Res. Commun., 105, p. 127; Smith, R.C., Nunn, V., (1984) Arch Biochem Biphys, 232, p. 348; Unnikrishnan, M.K., Rao, M.N.A., (1995) Pharmazie., 50, p. 490; Unnikrishnan, M.K., Rao, M.N.A., (1995) Mol. Cell. Biochem., 146, p. 35

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Y1 - 2000

N2 - 5 Amino salicylic acid (5-ASA) and several structurally-related analogs were tested for inhibitory role on nitrite-induced oxidation of hemoglobin in human blood-hemolysates. Results indicate a strong inhibitory role for 5-ASA in a concentration range of 0.2 to 0.8 mM. Although the inhibitory activity of 5-ASA was higher than other structural alalogs tested, the activity of curcumin was much higher at equimolar levels. PABA also showed some activity at similar concentrations although it was lower than that of 5-ASA. Benzoic acid showed marginal activity at lower concentrations. On the other hand, acetyl salicylic acid and salicylic acid increased the methemoglobin levels in hemolysate. The pro-oxidant activity was higher at higher concentrations for these compounds. Higher level of activity of 5-ASA is consistent with previous findings. As acetyl salicylic acid and 5-ASA are currently being prescribed, it is worth investigating their in vivo activity in order to establish novel and hitherto unexplored clinical applications.

AB - 5 Amino salicylic acid (5-ASA) and several structurally-related analogs were tested for inhibitory role on nitrite-induced oxidation of hemoglobin in human blood-hemolysates. Results indicate a strong inhibitory role for 5-ASA in a concentration range of 0.2 to 0.8 mM. Although the inhibitory activity of 5-ASA was higher than other structural alalogs tested, the activity of curcumin was much higher at equimolar levels. PABA also showed some activity at similar concentrations although it was lower than that of 5-ASA. Benzoic acid showed marginal activity at lower concentrations. On the other hand, acetyl salicylic acid and salicylic acid increased the methemoglobin levels in hemolysate. The pro-oxidant activity was higher at higher concentrations for these compounds. Higher level of activity of 5-ASA is consistent with previous findings. As acetyl salicylic acid and 5-ASA are currently being prescribed, it is worth investigating their in vivo activity in order to establish novel and hitherto unexplored clinical applications.

M3 - Article

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SP - 466

EP - 468

JO - Indian Journal of Pharmaceutical Sciences

JF - Indian Journal of Pharmaceutical Sciences

SN - 0250-474X

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