5-Aminosalicylic acid reverses endosulfan-induced testicular toxicity in male rats

A. Jaiswal, V. Kumar Parihar, M. Sudheer Kumar, S.D. Manjula, B.R. Krishnanand, R. Shanbhag, M.K. Unnikrishnan

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Pre-treatment with 5-aminosalicylic acid (5-ASA) significantly reduced sperm-shape abnormalities in endosulfan-treated rats. The number of abnormal sperm in the epididymis was markedly increased by endosulfan treatment but pre-treatment with 5-ASA kept these values close to normal. Treatment with 5-ASA at a dose of 25 mg/kg bw was more effective in reducing sperm-shape abnormality and sperm count than at a dose of 50 mg/kg bw. Endosulfan significantly increased the level of lactate dehydrogenase (LDH) in rats but a marked decrease was observed upon pre-treatment with 25 mg/kg bw 5-ASA. Changes in plasma testosterone levels were not significantly correlated with 5-ASA pre-treatment but histopathological analysis of seminiferous tubules and Leydig cells showed significant protection from endosulfan-induced tissue damage such as necrosis. The population of Sertoli cells increased and the lumen of the seminiferous tubules contained a greater number of spermatids. There was a corresponding increase in the number of Leydig cells. A curative study with 5-ASA showed a similar protection from endosulfan-induced toxicity and cellular damage, but the extent of protection was significantly lower. © 2005 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)50-59
Number of pages10
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume585
Issue number1-2
DOIs
Publication statusPublished - 2005

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Endosulfan
Mesalamine
Seminiferous Tubules
Sperm Count
Leydig Cells
Spermatozoa
Spermatids
Sertoli Cells
Epididymis
L-Lactate Dehydrogenase
Testosterone
Necrosis
Population

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Jaiswal, A., Kumar Parihar, V., Sudheer Kumar, M., Manjula, S. D., Krishnanand, B. R., Shanbhag, R., & Unnikrishnan, M. K. (2005). 5-Aminosalicylic acid reverses endosulfan-induced testicular toxicity in male rats. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 585(1-2), 50-59. https://doi.org/10.1016/j.mrgentox.2005.04.010
Jaiswal, A. ; Kumar Parihar, V. ; Sudheer Kumar, M. ; Manjula, S.D. ; Krishnanand, B.R. ; Shanbhag, R. ; Unnikrishnan, M.K. / 5-Aminosalicylic acid reverses endosulfan-induced testicular toxicity in male rats. In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2005 ; Vol. 585, No. 1-2. pp. 50-59.
@article{629704e189414bb293e2ca8d6d2de023,
title = "5-Aminosalicylic acid reverses endosulfan-induced testicular toxicity in male rats",
abstract = "Pre-treatment with 5-aminosalicylic acid (5-ASA) significantly reduced sperm-shape abnormalities in endosulfan-treated rats. The number of abnormal sperm in the epididymis was markedly increased by endosulfan treatment but pre-treatment with 5-ASA kept these values close to normal. Treatment with 5-ASA at a dose of 25 mg/kg bw was more effective in reducing sperm-shape abnormality and sperm count than at a dose of 50 mg/kg bw. Endosulfan significantly increased the level of lactate dehydrogenase (LDH) in rats but a marked decrease was observed upon pre-treatment with 25 mg/kg bw 5-ASA. Changes in plasma testosterone levels were not significantly correlated with 5-ASA pre-treatment but histopathological analysis of seminiferous tubules and Leydig cells showed significant protection from endosulfan-induced tissue damage such as necrosis. The population of Sertoli cells increased and the lumen of the seminiferous tubules contained a greater number of spermatids. There was a corresponding increase in the number of Leydig cells. A curative study with 5-ASA showed a similar protection from endosulfan-induced toxicity and cellular damage, but the extent of protection was significantly lower. {\circledC} 2005 Elsevier B.V. All rights reserved.",
author = "A. Jaiswal and {Kumar Parihar}, V. and {Sudheer Kumar}, M. and S.D. Manjula and B.R. Krishnanand and R. Shanbhag and M.K. Unnikrishnan",
note = "Cited By :18 Export Date: 10 November 2017 CODEN: MRGMF Correspondence Address: Unnikrishnan, M.K.; Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal 576104, India; email: cheruvaloor@yahoo.com Chemicals/CAS: acetylsalicylic acid, 493-53-8, 50-78-2, 53663-74-4, 53664-49-6, 63781-77-1; endosulfan, 115-29-7; lactate dehydrogenase, 9001-60-9; mesalazine, 89-57-6; testosterone, 58-22-0; Antidotes; Endosulfan, 115-29-7; Insecticides; L-Lactate Dehydrogenase, EC 1.1.1.27; Mesalamine, 89-57-6; Testosterone, 58-22-0 References: (1984) WHO Report International Programme on Chemical Safety, Environmental Health Criteria-40, Endosulfan, p. 28. , WHO, Geneva; Popov, V.B., Protasqvo, G.A., Radilov, A.S., Embryo and genotoxic effects of two endosulfan forms in the culture of rat and mouse pre and pos implantation embryos (1998) Oncogeny, 29, pp. 104-112; Wade, M.G., Desaulniers, D., Leingartner, K., Foster, W.G., Interactions between endosulfan and dieldrin on estrogen-mediated processes in vitro and in vivo (1997) Reprod. Toxicol., 11, pp. 791-798; Singh, S.K., Pandey, R.S., Effect of sub-chronic endosulfan exposures on plasma gonadotrophins, testosterone, testicular testosterone and enzymes of androgen biosynthesis in rat (1990) Indian J. Exp. Biol., 28, pp. 953-956; Chitra, K.C., Latchoumycandane, C., Mathur, P.P., Chronic effect of endosulfan on the testicular functions of rat (1999) Asian J. Androl., 1, pp. 203-206; Sinha, N., Narayan, R., Saxena, D.K., Endosulfan-induced biochemical changes in the Testis of rats (1995) Vet. Hum. Toxicol., 37, pp. 547-549; Kalender, S., Kalender, Y., Ogutcu, A., Uzunhisarcikli, M., Durak, D., Acikgoz, F., Endosulfan-induced cardiotoxicity and free radical metabolism in rats: The protective effect of vitamin e (2004) Toxicology, 202, pp. 227-235; Choudhary, N., Sharma, M., Verma, P., Joshi, S.C., Hepato and nephrotoxicity in rat exposed to endosulfan (2003) J. Environ. Biol., 24, pp. 305-308; Ramesh, A., Vijayalakshmi, A., Environmental exposure to residues after aerial spraying of endosulfan: Residues in cow milk, fish, water, soil and cashew leaf in Kasargode, Kerala, India (2002) Pest Manag. Sci., 58, pp. 1048-1054; Saiyed, H., Dewan, A., Bhatnagar, V., Shenoy, U., Shenoy, R., Rajmohan, H., Patel, K., Lakkad, B., Effect of endosulfan on male reproductive development (2003) Environ. Health Perspect., 111, pp. 1958-1962; Sanjay, K., Pesticide ban imposed after reports of congenital abnormalities (2002) BMJ, 325 (7360), p. 356; Anand, A.C., Kerala pesticide tragedy (2001) Natl. Med J. India, 14, pp. 123-124; Joshi, S., A curse called endosulfan: Kerala village blames aerial spraying of the pesticide endosulfan for unusual diseases (2001) Down Earth, 9 (19), pp. 28-35; Sanghi, R., Pillai, M.K., Jayalekshmi, T.R., Nair, A., Organochlorine and organophosphorus pesticide residues in breast milk from Bhopal, Madhya Pradesh, India (2003) Hum. Exp. Toxicol., 22, pp. 73-76; Miles Allen, M., Matthew Grisham, B., Antioxidant properties of aminosalicylates (1994) Methods Enzymol., 234, pp. 557-573; Sudheer Kumar, M., Unnikrishnan, M.K., Uma Devi, P., Effect of 5-ASA on radiation induced micronuclei in mouse bone marrow (2003) Mutat. Res., 527, pp. 7-14; Cann, P.A., Holdsworth, C.D., Reversal of male infertility on changing treatment from sulfasalazine to 5-amino salicylic acid (1984) Lancet, 1 (8386), p. 1119; Decker, T., Lohmann Matthes, M.L., A quick and simple method for the quantitation of lactate dehydogenase release in measurements of cellular cytotoxicity and tumour necrosis factor (TNF) activity (1998) J. Immunol. Methods, 115, pp. 61-69; Chen, A., Bookstein, J.J., Meldrum, D.R., Diagnosis of the of the testosterone secreting adrenal adenoma by selective venous catheterisation (1991) Fertil. Steril., 55, pp. 1202-1203; Wyrobek, A.J., Meistrich, M.L., Furrer, R., Bruce, W.R., Physical characteristics of mouse sperm nuclei (1976) Biophys. J., 16, pp. 811-825; Rambabu, J.P., Rao, M.B., Effect of an organochlorine and three organophosphate pesticides on glucose, glycogen, lipid, and protein contents in tissues of the freshwater snail Bellamya dissimilis (Muller) (1994) Bull. Environ. Contam. Toxicol., 53, pp. 142-148; Naqvi, S.M., Vaishnavi, V., Bioaccumulative potential and toxicity of endosulfan insecticide to non-target animals (1993) Comp. Biochem. Physiol. C, 105, pp. 347-361; Gledhill, B.L., Gledhill, M.P., Rigler, R., Ringertz, N.R., Changes in deoxyribonucleoprotein during spermiogenesis in the bull (1966) Exp. Cell Res., 41, pp. 652-665; Lam, D.M.K., Bruce, W.R., The biosynthesis of protamine during spermatogenesis of the mouse: Extraction, partial characterization, and site of synthesis (1971) J. Cell Physiol., 78, pp. 12-24; Loute, A.J., Dixon, G.H., Kinetics of phosphorylation and dephosphoryration of testis histones and their possible role in determining chromosomal structure (1973) Nat. New Biol., 243, pp. 164-168; Pryor, W.A., The involvement of free radicals in chemical carcinogenesis (1987) Anticarcinogenesis and Radiation Protection, , P.A. Cerutti O.F. Nygaard M.G. Simic Plenum New York; Wild, D., Mutagenecity study on organophosphate pesticides (1975) Mutat. Res., 32, pp. 133-150; Vuillaume, M., Reduced oxygen species mutation induction and cancer initiation (1987) Mutat. Res., 186, pp. 43-72; Pearson, D.C., Heuil, D.J., Meddings, J.B., The anti-oxidant properties of 5-aminosalicylic acid (1996) Free Radic. Biol. Med., 21, pp. 367-373; Eigenbrodt, E., Gerbracht, U., Mazurek, S., Presek, P., Friis, R., Carbohydrate metabolism and neoplasia: New perspectives for diagnosis and therapy (1994) Biochemical and Molecular Aspects of Selected Cancers, 2, pp. 311-385. , T.G. Pretlow, T.P. Pretlow (eds.), Academic Press, Inc; Wu, Y.W., Chik, C.L., Knazek, R.A., An in vitro and in vivo study of anti tumor effects of gossypol on human SW-13 adrenocortical carcinoma (1989) Cancer Res., 49, pp. 3754-3758; Shim, H., Dolde, C., Lewis, B.C., Wu, C.S., Dang, G., Jungmann, R.A., Dalla Favera, R., Dang, C.V., C-Myc transactivation of LDH-A: Implications for tumor metabolism and growth (1997) Proc. Natl. Acad. Sci. U.S.A., 94, pp. 6658-6663",
year = "2005",
doi = "10.1016/j.mrgentox.2005.04.010",
language = "English",
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pages = "50--59",
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number = "1-2",

}

Jaiswal, A, Kumar Parihar, V, Sudheer Kumar, M, Manjula, SD, Krishnanand, BR, Shanbhag, R & Unnikrishnan, MK 2005, '5-Aminosalicylic acid reverses endosulfan-induced testicular toxicity in male rats', Mutation Research - Genetic Toxicology and Environmental Mutagenesis, vol. 585, no. 1-2, pp. 50-59. https://doi.org/10.1016/j.mrgentox.2005.04.010

5-Aminosalicylic acid reverses endosulfan-induced testicular toxicity in male rats. / Jaiswal, A.; Kumar Parihar, V.; Sudheer Kumar, M.; Manjula, S.D.; Krishnanand, B.R.; Shanbhag, R.; Unnikrishnan, M.K.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 585, No. 1-2, 2005, p. 50-59.

Research output: Contribution to journalArticle

TY - JOUR

T1 - 5-Aminosalicylic acid reverses endosulfan-induced testicular toxicity in male rats

AU - Jaiswal, A.

AU - Kumar Parihar, V.

AU - Sudheer Kumar, M.

AU - Manjula, S.D.

AU - Krishnanand, B.R.

AU - Shanbhag, R.

AU - Unnikrishnan, M.K.

N1 - Cited By :18 Export Date: 10 November 2017 CODEN: MRGMF Correspondence Address: Unnikrishnan, M.K.; Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal 576104, India; email: cheruvaloor@yahoo.com Chemicals/CAS: acetylsalicylic acid, 493-53-8, 50-78-2, 53663-74-4, 53664-49-6, 63781-77-1; endosulfan, 115-29-7; lactate dehydrogenase, 9001-60-9; mesalazine, 89-57-6; testosterone, 58-22-0; Antidotes; Endosulfan, 115-29-7; Insecticides; L-Lactate Dehydrogenase, EC 1.1.1.27; Mesalamine, 89-57-6; Testosterone, 58-22-0 References: (1984) WHO Report International Programme on Chemical Safety, Environmental Health Criteria-40, Endosulfan, p. 28. , WHO, Geneva; Popov, V.B., Protasqvo, G.A., Radilov, A.S., Embryo and genotoxic effects of two endosulfan forms in the culture of rat and mouse pre and pos implantation embryos (1998) Oncogeny, 29, pp. 104-112; Wade, M.G., Desaulniers, D., Leingartner, K., Foster, W.G., Interactions between endosulfan and dieldrin on estrogen-mediated processes in vitro and in vivo (1997) Reprod. Toxicol., 11, pp. 791-798; Singh, S.K., Pandey, R.S., Effect of sub-chronic endosulfan exposures on plasma gonadotrophins, testosterone, testicular testosterone and enzymes of androgen biosynthesis in rat (1990) Indian J. Exp. Biol., 28, pp. 953-956; Chitra, K.C., Latchoumycandane, C., Mathur, P.P., Chronic effect of endosulfan on the testicular functions of rat (1999) Asian J. Androl., 1, pp. 203-206; Sinha, N., Narayan, R., Saxena, D.K., Endosulfan-induced biochemical changes in the Testis of rats (1995) Vet. Hum. Toxicol., 37, pp. 547-549; Kalender, S., Kalender, Y., Ogutcu, A., Uzunhisarcikli, M., Durak, D., Acikgoz, F., Endosulfan-induced cardiotoxicity and free radical metabolism in rats: The protective effect of vitamin e (2004) Toxicology, 202, pp. 227-235; Choudhary, N., Sharma, M., Verma, P., Joshi, S.C., Hepato and nephrotoxicity in rat exposed to endosulfan (2003) J. Environ. Biol., 24, pp. 305-308; Ramesh, A., Vijayalakshmi, A., Environmental exposure to residues after aerial spraying of endosulfan: Residues in cow milk, fish, water, soil and cashew leaf in Kasargode, Kerala, India (2002) Pest Manag. Sci., 58, pp. 1048-1054; Saiyed, H., Dewan, A., Bhatnagar, V., Shenoy, U., Shenoy, R., Rajmohan, H., Patel, K., Lakkad, B., Effect of endosulfan on male reproductive development (2003) Environ. Health Perspect., 111, pp. 1958-1962; Sanjay, K., Pesticide ban imposed after reports of congenital abnormalities (2002) BMJ, 325 (7360), p. 356; Anand, A.C., Kerala pesticide tragedy (2001) Natl. Med J. India, 14, pp. 123-124; Joshi, S., A curse called endosulfan: Kerala village blames aerial spraying of the pesticide endosulfan for unusual diseases (2001) Down Earth, 9 (19), pp. 28-35; Sanghi, R., Pillai, M.K., Jayalekshmi, T.R., Nair, A., Organochlorine and organophosphorus pesticide residues in breast milk from Bhopal, Madhya Pradesh, India (2003) Hum. Exp. Toxicol., 22, pp. 73-76; Miles Allen, M., Matthew Grisham, B., Antioxidant properties of aminosalicylates (1994) Methods Enzymol., 234, pp. 557-573; Sudheer Kumar, M., Unnikrishnan, M.K., Uma Devi, P., Effect of 5-ASA on radiation induced micronuclei in mouse bone marrow (2003) Mutat. Res., 527, pp. 7-14; Cann, P.A., Holdsworth, C.D., Reversal of male infertility on changing treatment from sulfasalazine to 5-amino salicylic acid (1984) Lancet, 1 (8386), p. 1119; Decker, T., Lohmann Matthes, M.L., A quick and simple method for the quantitation of lactate dehydogenase release in measurements of cellular cytotoxicity and tumour necrosis factor (TNF) activity (1998) J. Immunol. Methods, 115, pp. 61-69; Chen, A., Bookstein, J.J., Meldrum, D.R., Diagnosis of the of the testosterone secreting adrenal adenoma by selective venous catheterisation (1991) Fertil. Steril., 55, pp. 1202-1203; Wyrobek, A.J., Meistrich, M.L., Furrer, R., Bruce, W.R., Physical characteristics of mouse sperm nuclei (1976) Biophys. J., 16, pp. 811-825; Rambabu, J.P., Rao, M.B., Effect of an organochlorine and three organophosphate pesticides on glucose, glycogen, lipid, and protein contents in tissues of the freshwater snail Bellamya dissimilis (Muller) (1994) Bull. Environ. Contam. Toxicol., 53, pp. 142-148; Naqvi, S.M., Vaishnavi, V., Bioaccumulative potential and toxicity of endosulfan insecticide to non-target animals (1993) Comp. Biochem. Physiol. C, 105, pp. 347-361; Gledhill, B.L., Gledhill, M.P., Rigler, R., Ringertz, N.R., Changes in deoxyribonucleoprotein during spermiogenesis in the bull (1966) Exp. Cell Res., 41, pp. 652-665; Lam, D.M.K., Bruce, W.R., The biosynthesis of protamine during spermatogenesis of the mouse: Extraction, partial characterization, and site of synthesis (1971) J. Cell Physiol., 78, pp. 12-24; Loute, A.J., Dixon, G.H., Kinetics of phosphorylation and dephosphoryration of testis histones and their possible role in determining chromosomal structure (1973) Nat. New Biol., 243, pp. 164-168; Pryor, W.A., The involvement of free radicals in chemical carcinogenesis (1987) Anticarcinogenesis and Radiation Protection, , P.A. Cerutti O.F. Nygaard M.G. Simic Plenum New York; Wild, D., Mutagenecity study on organophosphate pesticides (1975) Mutat. Res., 32, pp. 133-150; Vuillaume, M., Reduced oxygen species mutation induction and cancer initiation (1987) Mutat. Res., 186, pp. 43-72; Pearson, D.C., Heuil, D.J., Meddings, J.B., The anti-oxidant properties of 5-aminosalicylic acid (1996) Free Radic. Biol. Med., 21, pp. 367-373; Eigenbrodt, E., Gerbracht, U., Mazurek, S., Presek, P., Friis, R., Carbohydrate metabolism and neoplasia: New perspectives for diagnosis and therapy (1994) Biochemical and Molecular Aspects of Selected Cancers, 2, pp. 311-385. , T.G. Pretlow, T.P. Pretlow (eds.), Academic Press, Inc; Wu, Y.W., Chik, C.L., Knazek, R.A., An in vitro and in vivo study of anti tumor effects of gossypol on human SW-13 adrenocortical carcinoma (1989) Cancer Res., 49, pp. 3754-3758; Shim, H., Dolde, C., Lewis, B.C., Wu, C.S., Dang, G., Jungmann, R.A., Dalla Favera, R., Dang, C.V., C-Myc transactivation of LDH-A: Implications for tumor metabolism and growth (1997) Proc. Natl. Acad. Sci. U.S.A., 94, pp. 6658-6663

PY - 2005

Y1 - 2005

N2 - Pre-treatment with 5-aminosalicylic acid (5-ASA) significantly reduced sperm-shape abnormalities in endosulfan-treated rats. The number of abnormal sperm in the epididymis was markedly increased by endosulfan treatment but pre-treatment with 5-ASA kept these values close to normal. Treatment with 5-ASA at a dose of 25 mg/kg bw was more effective in reducing sperm-shape abnormality and sperm count than at a dose of 50 mg/kg bw. Endosulfan significantly increased the level of lactate dehydrogenase (LDH) in rats but a marked decrease was observed upon pre-treatment with 25 mg/kg bw 5-ASA. Changes in plasma testosterone levels were not significantly correlated with 5-ASA pre-treatment but histopathological analysis of seminiferous tubules and Leydig cells showed significant protection from endosulfan-induced tissue damage such as necrosis. The population of Sertoli cells increased and the lumen of the seminiferous tubules contained a greater number of spermatids. There was a corresponding increase in the number of Leydig cells. A curative study with 5-ASA showed a similar protection from endosulfan-induced toxicity and cellular damage, but the extent of protection was significantly lower. © 2005 Elsevier B.V. All rights reserved.

AB - Pre-treatment with 5-aminosalicylic acid (5-ASA) significantly reduced sperm-shape abnormalities in endosulfan-treated rats. The number of abnormal sperm in the epididymis was markedly increased by endosulfan treatment but pre-treatment with 5-ASA kept these values close to normal. Treatment with 5-ASA at a dose of 25 mg/kg bw was more effective in reducing sperm-shape abnormality and sperm count than at a dose of 50 mg/kg bw. Endosulfan significantly increased the level of lactate dehydrogenase (LDH) in rats but a marked decrease was observed upon pre-treatment with 25 mg/kg bw 5-ASA. Changes in plasma testosterone levels were not significantly correlated with 5-ASA pre-treatment but histopathological analysis of seminiferous tubules and Leydig cells showed significant protection from endosulfan-induced tissue damage such as necrosis. The population of Sertoli cells increased and the lumen of the seminiferous tubules contained a greater number of spermatids. There was a corresponding increase in the number of Leydig cells. A curative study with 5-ASA showed a similar protection from endosulfan-induced toxicity and cellular damage, but the extent of protection was significantly lower. © 2005 Elsevier B.V. All rights reserved.

U2 - 10.1016/j.mrgentox.2005.04.010

DO - 10.1016/j.mrgentox.2005.04.010

M3 - Article

VL - 585

SP - 50

EP - 59

JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

SN - 1383-5718

IS - 1-2

ER -