A colorimetric method for the estimation of serum glycated proteins based on differential reduction of free and bound glucose by sodium borohydride

Anuradha Kumar, Pragna Rao, T. N. Pattabiraman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A new colorimetric method based on the phenol-sulfuric acid reaction is described for the estimation of serum glycated proteins by the differential reduction of free glucose and hexose bound nonenzymatically with 2.0 and 20 mg of NaBH4 in 0.02 ml of serum, respectively, at room temperature for 15 min. The values (μg hexose/mg protein) in control subjects (n = 60) and diabetics (n = 90) were estimated to be 5.60 ± 0.85 and 10.8 ± 1.6, respectively. The increase was highly significant (P < 0.001) in diabetics. The serum glycated protein levels correlate well with fasting blood sugar values (r = 0.77, P < 0.001, n = 25). There was also a highly significant correlation between glycated protein level and glycated albumin value in individual serum samples (r = 0.85, P < 0.001, n = 25). Values of borohydride reducible glyco-groups bound to serum proteins also correlated well with serum glycated protein levels (r = 0.96, p < 0.001, n = 20) determined by the thiobarbituric acid assay method. The method is found to be simple and rapid, with a coefficient of variations of ±3.8%.

Original languageEnglish
Pages (from-to)296-304
Number of pages9
JournalBiochemical Medicine and Metabolic Biology
Volume39
Issue number3
DOIs
Publication statusPublished - 1988

Fingerprint

Blood Proteins
Glucose
Hexoses
Proteins
Borohydrides
Phenol
Serum
Blood Glucose
Fasting
Assays
Temperature
sodium borohydride

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

@article{5fcb394a3da54a08ba438b7f770035ef,
title = "A colorimetric method for the estimation of serum glycated proteins based on differential reduction of free and bound glucose by sodium borohydride",
abstract = "A new colorimetric method based on the phenol-sulfuric acid reaction is described for the estimation of serum glycated proteins by the differential reduction of free glucose and hexose bound nonenzymatically with 2.0 and 20 mg of NaBH4 in 0.02 ml of serum, respectively, at room temperature for 15 min. The values (μg hexose/mg protein) in control subjects (n = 60) and diabetics (n = 90) were estimated to be 5.60 ± 0.85 and 10.8 ± 1.6, respectively. The increase was highly significant (P < 0.001) in diabetics. The serum glycated protein levels correlate well with fasting blood sugar values (r = 0.77, P < 0.001, n = 25). There was also a highly significant correlation between glycated protein level and glycated albumin value in individual serum samples (r = 0.85, P < 0.001, n = 25). Values of borohydride reducible glyco-groups bound to serum proteins also correlated well with serum glycated protein levels (r = 0.96, p < 0.001, n = 20) determined by the thiobarbituric acid assay method. The method is found to be simple and rapid, with a coefficient of variations of ±3.8{\%}.",
author = "Anuradha Kumar and Pragna Rao and Pattabiraman, {T. N.}",
year = "1988",
doi = "10.1016/0885-4505(88)90089-8",
language = "English",
volume = "39",
pages = "296--304",
journal = "Molecular Genetics and Metabolism",
issn = "1096-7192",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - A colorimetric method for the estimation of serum glycated proteins based on differential reduction of free and bound glucose by sodium borohydride

AU - Kumar, Anuradha

AU - Rao, Pragna

AU - Pattabiraman, T. N.

PY - 1988

Y1 - 1988

N2 - A new colorimetric method based on the phenol-sulfuric acid reaction is described for the estimation of serum glycated proteins by the differential reduction of free glucose and hexose bound nonenzymatically with 2.0 and 20 mg of NaBH4 in 0.02 ml of serum, respectively, at room temperature for 15 min. The values (μg hexose/mg protein) in control subjects (n = 60) and diabetics (n = 90) were estimated to be 5.60 ± 0.85 and 10.8 ± 1.6, respectively. The increase was highly significant (P < 0.001) in diabetics. The serum glycated protein levels correlate well with fasting blood sugar values (r = 0.77, P < 0.001, n = 25). There was also a highly significant correlation between glycated protein level and glycated albumin value in individual serum samples (r = 0.85, P < 0.001, n = 25). Values of borohydride reducible glyco-groups bound to serum proteins also correlated well with serum glycated protein levels (r = 0.96, p < 0.001, n = 20) determined by the thiobarbituric acid assay method. The method is found to be simple and rapid, with a coefficient of variations of ±3.8%.

AB - A new colorimetric method based on the phenol-sulfuric acid reaction is described for the estimation of serum glycated proteins by the differential reduction of free glucose and hexose bound nonenzymatically with 2.0 and 20 mg of NaBH4 in 0.02 ml of serum, respectively, at room temperature for 15 min. The values (μg hexose/mg protein) in control subjects (n = 60) and diabetics (n = 90) were estimated to be 5.60 ± 0.85 and 10.8 ± 1.6, respectively. The increase was highly significant (P < 0.001) in diabetics. The serum glycated protein levels correlate well with fasting blood sugar values (r = 0.77, P < 0.001, n = 25). There was also a highly significant correlation between glycated protein level and glycated albumin value in individual serum samples (r = 0.85, P < 0.001, n = 25). Values of borohydride reducible glyco-groups bound to serum proteins also correlated well with serum glycated protein levels (r = 0.96, p < 0.001, n = 20) determined by the thiobarbituric acid assay method. The method is found to be simple and rapid, with a coefficient of variations of ±3.8%.

UR - http://www.scopus.com/inward/record.url?scp=0023952011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023952011&partnerID=8YFLogxK

U2 - 10.1016/0885-4505(88)90089-8

DO - 10.1016/0885-4505(88)90089-8

M3 - Article

VL - 39

SP - 296

EP - 304

JO - Molecular Genetics and Metabolism

JF - Molecular Genetics and Metabolism

SN - 1096-7192

IS - 3

ER -