A comparative evaluation of antimicrobial efficacy of hydroalcoholic extract of cymbopogon citratus and chlorhexidine gluconate against candida albicans and enterococcus faecalis - An in vitro study

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The aim of present in vitro study was to investigate antimicrobial efficacy of hydroalcoholic extract of Cymbopogon citratus and 2% chlorhexidine gluconate solution against Candida albicans and Enterococcus faecalis. Punch well method using lawn cultures of the C. albicans and E. faecalis was performed to determine the antimicrobial efficacy in the intial stage. The inoculae of these organisms were used to make the lawn culture on Muller Hinton agar plates. Wells were prepared in these lawn cultures and filled with the test agents. The agar plates were kept for incubation at 370 C and zone of inhibition was examined after 24 to 48 hours. Further, Direct Contact method was performed to establish the temporal relation between the pre determined concentration of the extract and the microbicidal efficacy. Gaussian test was employed as test of significance (p<0.05). The results suggest that there was a significant difference in the antimicrobial activity between C. citratus and chlorhexidine gluconate against C. albicans and E. faecalis. C. citratus had a significant antimicrobial efficacy against C. albicans than E. faecalis whereas, chlorhexidine gluconate was more effective against E. faecalis than C. albicans. Clinical Significance: C. Citratus can be used as a potent antifungal agent against C. albicans.

Original languageEnglish
Title of host publicationClinical Dentistry Research Compendium
PublisherNova Science Publishers Inc
Pages219-225
Number of pages7
ISBN (Print)9781622575749
Publication statusPublished - 01-01-2013

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Cymbopogon
Enterococcus faecalis
Candida albicans
Agar
Antifungal Agents
chlorhexidine gluconate
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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title = "A comparative evaluation of antimicrobial efficacy of hydroalcoholic extract of cymbopogon citratus and chlorhexidine gluconate against candida albicans and enterococcus faecalis - An in vitro study",
abstract = "The aim of present in vitro study was to investigate antimicrobial efficacy of hydroalcoholic extract of Cymbopogon citratus and 2{\%} chlorhexidine gluconate solution against Candida albicans and Enterococcus faecalis. Punch well method using lawn cultures of the C. albicans and E. faecalis was performed to determine the antimicrobial efficacy in the intial stage. The inoculae of these organisms were used to make the lawn culture on Muller Hinton agar plates. Wells were prepared in these lawn cultures and filled with the test agents. The agar plates were kept for incubation at 370 C and zone of inhibition was examined after 24 to 48 hours. Further, Direct Contact method was performed to establish the temporal relation between the pre determined concentration of the extract and the microbicidal efficacy. Gaussian test was employed as test of significance (p<0.05). The results suggest that there was a significant difference in the antimicrobial activity between C. citratus and chlorhexidine gluconate against C. albicans and E. faecalis. C. citratus had a significant antimicrobial efficacy against C. albicans than E. faecalis whereas, chlorhexidine gluconate was more effective against E. faecalis than C. albicans. Clinical Significance: C. Citratus can be used as a potent antifungal agent against C. albicans.",
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A comparative evaluation of antimicrobial efficacy of hydroalcoholic extract of cymbopogon citratus and chlorhexidine gluconate against candida albicans and enterococcus faecalis - An in vitro study. / Ballal, N. Vasudev; Prakash, P. Y.; Bhata, K. S.

Clinical Dentistry Research Compendium. Nova Science Publishers Inc, 2013. p. 219-225.

Research output: Chapter in Book/Report/Conference proceedingChapter

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