A comparative evaluation of antimicrobial efficacy of hydroalcoholic extract of cymbopogon citratus and chlorhexidine gluconate against candida albicans and enterococcus faecalis - an in vitro study

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Abstract

The aim of present in vitro study was to investigate antimicrobial efficacy of hydroalcoholic extract of Cymbopogon citratus and 2% chlorhexidine gluconate solution against Candida albicans and Enterococcus faecalis. Punch well method using lawn cultures of the C. albicans and E. faecalis was performed to determine the antimicrobial efficacy in the intial stage. The inoculae of these organisms were used to make the lawn culture on Muller Hinton agar plates. Wells were prepared in these lawn cultures and filled with the test agents. The agar plates were kept for incubation at 370 C and zone of inhibition was examined after 24 to 48 hours. Further, Direct Contact method was performed to establish the temporal relation between the pre determined concentration of the extract and the microbicidal efficacy. Gaussian test was employed as test of significance (p<0.05). The results suggest that there was a significant difference in the antimicrobial activity between C. citratus and chlorhexidine gluconate against C. albicans and E. faecalis. C. citratus had a significant antimicrobial efficacy against C. albicans than E. faecalis whereas, chlorhexidine gluconate was more effective against E. faecalis than C. albicans.

Original languageEnglish
Pages (from-to)221-227
Number of pages7
JournalInternational Journal of Clinical Dentistry
Volume4
Issue number3
Publication statusPublished - 10-08-2011

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Cymbopogon
Enterococcus faecalis
Candida albicans
Agar
In Vitro Techniques
chlorhexidine gluconate

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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title = "A comparative evaluation of antimicrobial efficacy of hydroalcoholic extract of cymbopogon citratus and chlorhexidine gluconate against candida albicans and enterococcus faecalis - an in vitro study",
abstract = "The aim of present in vitro study was to investigate antimicrobial efficacy of hydroalcoholic extract of Cymbopogon citratus and 2{\%} chlorhexidine gluconate solution against Candida albicans and Enterococcus faecalis. Punch well method using lawn cultures of the C. albicans and E. faecalis was performed to determine the antimicrobial efficacy in the intial stage. The inoculae of these organisms were used to make the lawn culture on Muller Hinton agar plates. Wells were prepared in these lawn cultures and filled with the test agents. The agar plates were kept for incubation at 370 C and zone of inhibition was examined after 24 to 48 hours. Further, Direct Contact method was performed to establish the temporal relation between the pre determined concentration of the extract and the microbicidal efficacy. Gaussian test was employed as test of significance (p<0.05). The results suggest that there was a significant difference in the antimicrobial activity between C. citratus and chlorhexidine gluconate against C. albicans and E. faecalis. C. citratus had a significant antimicrobial efficacy against C. albicans than E. faecalis whereas, chlorhexidine gluconate was more effective against E. faecalis than C. albicans.",
author = "Ballal, {N. Vasudev} and Prakash, {P. Y.} and Bhat, {K. S.}",
year = "2011",
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T1 - A comparative evaluation of antimicrobial efficacy of hydroalcoholic extract of cymbopogon citratus and chlorhexidine gluconate against candida albicans and enterococcus faecalis - an in vitro study

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AU - Prakash, P. Y.

AU - Bhat, K. S.

PY - 2011/8/10

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N2 - The aim of present in vitro study was to investigate antimicrobial efficacy of hydroalcoholic extract of Cymbopogon citratus and 2% chlorhexidine gluconate solution against Candida albicans and Enterococcus faecalis. Punch well method using lawn cultures of the C. albicans and E. faecalis was performed to determine the antimicrobial efficacy in the intial stage. The inoculae of these organisms were used to make the lawn culture on Muller Hinton agar plates. Wells were prepared in these lawn cultures and filled with the test agents. The agar plates were kept for incubation at 370 C and zone of inhibition was examined after 24 to 48 hours. Further, Direct Contact method was performed to establish the temporal relation between the pre determined concentration of the extract and the microbicidal efficacy. Gaussian test was employed as test of significance (p<0.05). The results suggest that there was a significant difference in the antimicrobial activity between C. citratus and chlorhexidine gluconate against C. albicans and E. faecalis. C. citratus had a significant antimicrobial efficacy against C. albicans than E. faecalis whereas, chlorhexidine gluconate was more effective against E. faecalis than C. albicans.

AB - The aim of present in vitro study was to investigate antimicrobial efficacy of hydroalcoholic extract of Cymbopogon citratus and 2% chlorhexidine gluconate solution against Candida albicans and Enterococcus faecalis. Punch well method using lawn cultures of the C. albicans and E. faecalis was performed to determine the antimicrobial efficacy in the intial stage. The inoculae of these organisms were used to make the lawn culture on Muller Hinton agar plates. Wells were prepared in these lawn cultures and filled with the test agents. The agar plates were kept for incubation at 370 C and zone of inhibition was examined after 24 to 48 hours. Further, Direct Contact method was performed to establish the temporal relation between the pre determined concentration of the extract and the microbicidal efficacy. Gaussian test was employed as test of significance (p<0.05). The results suggest that there was a significant difference in the antimicrobial activity between C. citratus and chlorhexidine gluconate against C. albicans and E. faecalis. C. citratus had a significant antimicrobial efficacy against C. albicans than E. faecalis whereas, chlorhexidine gluconate was more effective against E. faecalis than C. albicans.

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