Abstract

Objective: The objective of this study was to compare aqueous solutions of ethylenediaminetetraacetic acid (EDTA) with that of maleic acid (MA) for their cytotoxic effect on Chinese hamster fibroblasts (V79) cells growing in vitro. Study design: Exponentially growing V79 cells were treated with various concentrations of EDTA (0.05% to 1.0%) or MA (0.05% to 1.0%) alone for 30 minutes. After treatment, the media was removed, cells were trypsinized, and the cytotoxic effect of EDTA or MA was analyzed by Pratt Willis test and MTT assay. Similarly surviving fraction (clonogenic assay) was performed by treating the V79 cells with different concentrations of EDTA (0.0025% to 0.25%) or MA (0.025% to 0.25%) for 30 minutes. The statistical significance between the various groups was evaluated using the one-way analysis of variance (ANOVA) and Student t test (unpaired) for 2 group comparisons. Results: There was a significant (P < .01) decrease in the cell viability in a dose-dependent manner indicating the cytotoxic effect of both EDTA and MA when compared with the control group. However, all the dilutions of EDTA were significantly (P < .01) more cytotoxic over that of MA in all 3 assays. Conclusion: This study for the first time, clearly demonstrated the significantly less toxic effect of MA at a comparable dose of EDTA, suggesting its potential for use as root canal irrigant.

Original languageEnglish
Pages (from-to)633-638
Number of pages6
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology
Volume108
Issue number4
DOIs
Publication statusPublished - 10-2009

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Root Canal Irrigants
Edetic Acid
Poisons
Cricetulus
In Vitro Techniques
maleic acid
Cell Survival
Analysis of Variance
Fibroblasts
Students
Control Groups

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Surgery
  • Dentistry(all)
  • Oral Surgery

Cite this

@article{cb800bcc4bd4442f8fbcfc90dc8a8100,
title = "A comparative in vitro evaluation of cytotoxic effects of EDTA and maleic acid: Root canal irrigants",
abstract = "Objective: The objective of this study was to compare aqueous solutions of ethylenediaminetetraacetic acid (EDTA) with that of maleic acid (MA) for their cytotoxic effect on Chinese hamster fibroblasts (V79) cells growing in vitro. Study design: Exponentially growing V79 cells were treated with various concentrations of EDTA (0.05{\%} to 1.0{\%}) or MA (0.05{\%} to 1.0{\%}) alone for 30 minutes. After treatment, the media was removed, cells were trypsinized, and the cytotoxic effect of EDTA or MA was analyzed by Pratt Willis test and MTT assay. Similarly surviving fraction (clonogenic assay) was performed by treating the V79 cells with different concentrations of EDTA (0.0025{\%} to 0.25{\%}) or MA (0.025{\%} to 0.25{\%}) for 30 minutes. The statistical significance between the various groups was evaluated using the one-way analysis of variance (ANOVA) and Student t test (unpaired) for 2 group comparisons. Results: There was a significant (P < .01) decrease in the cell viability in a dose-dependent manner indicating the cytotoxic effect of both EDTA and MA when compared with the control group. However, all the dilutions of EDTA were significantly (P < .01) more cytotoxic over that of MA in all 3 assays. Conclusion: This study for the first time, clearly demonstrated the significantly less toxic effect of MA at a comparable dose of EDTA, suggesting its potential for use as root canal irrigant.",
author = "Ballal, {N. Vasudev} and M. Kundabala and Seetharama Bhat and Nageshwar Rao and Rao, {B. S Satish}",
year = "2009",
month = "10",
doi = "10.1016/j.tripleo.2009.05.039",
language = "English",
volume = "108",
pages = "633--638",
journal = "Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology",
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TY - JOUR

T1 - A comparative in vitro evaluation of cytotoxic effects of EDTA and maleic acid

T2 - Root canal irrigants

AU - Ballal, N. Vasudev

AU - Kundabala, M.

AU - Bhat, Seetharama

AU - Rao, Nageshwar

AU - Rao, B. S Satish

PY - 2009/10

Y1 - 2009/10

N2 - Objective: The objective of this study was to compare aqueous solutions of ethylenediaminetetraacetic acid (EDTA) with that of maleic acid (MA) for their cytotoxic effect on Chinese hamster fibroblasts (V79) cells growing in vitro. Study design: Exponentially growing V79 cells were treated with various concentrations of EDTA (0.05% to 1.0%) or MA (0.05% to 1.0%) alone for 30 minutes. After treatment, the media was removed, cells were trypsinized, and the cytotoxic effect of EDTA or MA was analyzed by Pratt Willis test and MTT assay. Similarly surviving fraction (clonogenic assay) was performed by treating the V79 cells with different concentrations of EDTA (0.0025% to 0.25%) or MA (0.025% to 0.25%) for 30 minutes. The statistical significance between the various groups was evaluated using the one-way analysis of variance (ANOVA) and Student t test (unpaired) for 2 group comparisons. Results: There was a significant (P < .01) decrease in the cell viability in a dose-dependent manner indicating the cytotoxic effect of both EDTA and MA when compared with the control group. However, all the dilutions of EDTA were significantly (P < .01) more cytotoxic over that of MA in all 3 assays. Conclusion: This study for the first time, clearly demonstrated the significantly less toxic effect of MA at a comparable dose of EDTA, suggesting its potential for use as root canal irrigant.

AB - Objective: The objective of this study was to compare aqueous solutions of ethylenediaminetetraacetic acid (EDTA) with that of maleic acid (MA) for their cytotoxic effect on Chinese hamster fibroblasts (V79) cells growing in vitro. Study design: Exponentially growing V79 cells were treated with various concentrations of EDTA (0.05% to 1.0%) or MA (0.05% to 1.0%) alone for 30 minutes. After treatment, the media was removed, cells were trypsinized, and the cytotoxic effect of EDTA or MA was analyzed by Pratt Willis test and MTT assay. Similarly surviving fraction (clonogenic assay) was performed by treating the V79 cells with different concentrations of EDTA (0.0025% to 0.25%) or MA (0.025% to 0.25%) for 30 minutes. The statistical significance between the various groups was evaluated using the one-way analysis of variance (ANOVA) and Student t test (unpaired) for 2 group comparisons. Results: There was a significant (P < .01) decrease in the cell viability in a dose-dependent manner indicating the cytotoxic effect of both EDTA and MA when compared with the control group. However, all the dilutions of EDTA were significantly (P < .01) more cytotoxic over that of MA in all 3 assays. Conclusion: This study for the first time, clearly demonstrated the significantly less toxic effect of MA at a comparable dose of EDTA, suggesting its potential for use as root canal irrigant.

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