A comparative study of in vivo efficacy of currently used antibiotics in murine model of klebsiella pneumonia

Preethi G. Pai, C. H. Anoop, P. V. Mirshad, Gopalkrishna Bhat, Shalini M. Shenoy, K. V. Ramesh

Research output: Contribution to journalArticle

Abstract

A comparative in-vivo efficacy of commonly used antibiotics was evaluated in the murine model of Klebsiella pneumonia. Six groups of immunosuppressed (cyclophosphamide 80mg/kg i.p. on -4 and -1 day) female Wistar rats (n=6-12) were employed to induce pneumonia. Bacterial inoculum of strength 1x 107 CFU/ml of Klebsiella pneumoniae strain isolated from sputum in the Department of Microbiology, Kasturba Medical College, Mangalore was injected intratracheally. Gentamicin (0.032g/kg body weight/day), Levofloxacin (0.036g/kg body weight/12hrly), Cefepime (0.18 g/kg body weight/12hrly), Aztreonam (0.18g/kg body weight/8hrly) and Meropenem (0.09 mg/kg body weight/8hrly) were administered i.p. within 4 hours following bacterial inoculation and continued for 5 days. On the sixth day, rats were sacrificed and the pneumonic lung tissues were used for bacterial count and histopathological studies. Levofloxacin significantly offered protection in Klebsiella pneumonia. Aztreonam seems to be a better choice to Cefepime and Gentamicin. Cefepime is found to be an effective antibiotic whereas Gentamicin is not effective against the local strains of K.pneumoniae. Thus in-vivo efficacy of antibiotic data may help to substantiate the choice of antibiotic against local strains of pneumopathogens. Also, these results may ensure more scientific authenticity than in-vitro bacteriological investigation reports.

Original languageEnglish
Pages (from-to)224-228
Number of pages5
JournalAsian Journal of Pharmaceutical and Clinical Research
Volume3
Issue number3
Publication statusPublished - 07-2010

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Klebsiella pneumoniae
Body Weight
Anti-Bacterial Agents
Gentamicins
Aztreonam
Levofloxacin
meropenem
Pneumonia
Lung
Bacterial Load
Microbiology
Sputum
Cyclophosphamide
Wistar Rats
cefepime

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science
  • Pharmacology (medical)

Cite this

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title = "A comparative study of in vivo efficacy of currently used antibiotics in murine model of klebsiella pneumonia",
abstract = "A comparative in-vivo efficacy of commonly used antibiotics was evaluated in the murine model of Klebsiella pneumonia. Six groups of immunosuppressed (cyclophosphamide 80mg/kg i.p. on -4 and -1 day) female Wistar rats (n=6-12) were employed to induce pneumonia. Bacterial inoculum of strength 1x 107 CFU/ml of Klebsiella pneumoniae strain isolated from sputum in the Department of Microbiology, Kasturba Medical College, Mangalore was injected intratracheally. Gentamicin (0.032g/kg body weight/day), Levofloxacin (0.036g/kg body weight/12hrly), Cefepime (0.18 g/kg body weight/12hrly), Aztreonam (0.18g/kg body weight/8hrly) and Meropenem (0.09 mg/kg body weight/8hrly) were administered i.p. within 4 hours following bacterial inoculation and continued for 5 days. On the sixth day, rats were sacrificed and the pneumonic lung tissues were used for bacterial count and histopathological studies. Levofloxacin significantly offered protection in Klebsiella pneumonia. Aztreonam seems to be a better choice to Cefepime and Gentamicin. Cefepime is found to be an effective antibiotic whereas Gentamicin is not effective against the local strains of K.pneumoniae. Thus in-vivo efficacy of antibiotic data may help to substantiate the choice of antibiotic against local strains of pneumopathogens. Also, these results may ensure more scientific authenticity than in-vitro bacteriological investigation reports.",
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A comparative study of in vivo efficacy of currently used antibiotics in murine model of klebsiella pneumonia. / Pai, Preethi G.; Anoop, C. H.; Mirshad, P. V.; Bhat, Gopalkrishna; Shenoy, Shalini M.; Ramesh, K. V.

In: Asian Journal of Pharmaceutical and Clinical Research, Vol. 3, No. 3, 07.2010, p. 224-228.

Research output: Contribution to journalArticle

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