A comparative study of Michel's medium versus honey as a transport medium for skin specimens prior to direct immunofluorescence microscopy and antigen mapping

Subraminiam Kumudhini, Sathish Pai, Chythra Rao, Raghavendra Rao

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Abstract

Background: Michel's medium (MM) is currently the recommended transport medium for skin biopsy specimens prior to direct immunofluorescence (DIF) microscopy. Objective: To compare the utility of honey with that of MM as a transport medium for skin biopsy specimens used for DIF and antigen mapping. Methods: Group I consisted of 45 freshly-taken skin specimens earmarked for DIF testing. It was divided into three groups (A, B and C), each containing 15 specimens. Biopsy specimens were sliced into two, one each for MM and honey. Samples in group A were processed at the end of week 1 while those in group B and C were processed at the end of weeks 2 and 4, respectively. Group II consisted of five specimens of epidermolysis bullosa (EB) which was further divided into three groups; two specimens were processed for antigen mapping at the end of week 1, while others were processed at the end of week 2 (two specimens) and 4 (one specimen). Results: Sensitivity of honey as a transport medium for skin biopsy specimens was 100%, 92.6% and 53.8% at weeks 1, 2 and 4, respectively. The antigen mapping was positive in all specimens. Conclusion: Utility of honey was comparable to MM for DIF samples tested at weeks 1 and 2 but was lower at week 4.

Original languageEnglish
JournalJournal of Cutaneous Pathology
DOIs
Publication statusPublished - 01-01-2019

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Direct Fluorescent Antibody Technique
Honey
Fluorescence Microscopy
Biopsy
Antigens
Skin
Epidermolysis Bullosa

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Dermatology

Cite this

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title = "A comparative study of Michel's medium versus honey as a transport medium for skin specimens prior to direct immunofluorescence microscopy and antigen mapping",
abstract = "Background: Michel's medium (MM) is currently the recommended transport medium for skin biopsy specimens prior to direct immunofluorescence (DIF) microscopy. Objective: To compare the utility of honey with that of MM as a transport medium for skin biopsy specimens used for DIF and antigen mapping. Methods: Group I consisted of 45 freshly-taken skin specimens earmarked for DIF testing. It was divided into three groups (A, B and C), each containing 15 specimens. Biopsy specimens were sliced into two, one each for MM and honey. Samples in group A were processed at the end of week 1 while those in group B and C were processed at the end of weeks 2 and 4, respectively. Group II consisted of five specimens of epidermolysis bullosa (EB) which was further divided into three groups; two specimens were processed for antigen mapping at the end of week 1, while others were processed at the end of week 2 (two specimens) and 4 (one specimen). Results: Sensitivity of honey as a transport medium for skin biopsy specimens was 100{\%}, 92.6{\%} and 53.8{\%} at weeks 1, 2 and 4, respectively. The antigen mapping was positive in all specimens. Conclusion: Utility of honey was comparable to MM for DIF samples tested at weeks 1 and 2 but was lower at week 4.",
author = "Subraminiam Kumudhini and Sathish Pai and Chythra Rao and Raghavendra Rao",
year = "2019",
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language = "English",
journal = "Journal of Cutaneous Pathology",
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T1 - A comparative study of Michel's medium versus honey as a transport medium for skin specimens prior to direct immunofluorescence microscopy and antigen mapping

AU - Kumudhini, Subraminiam

AU - Pai, Sathish

AU - Rao, Chythra

AU - Rao, Raghavendra

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: Michel's medium (MM) is currently the recommended transport medium for skin biopsy specimens prior to direct immunofluorescence (DIF) microscopy. Objective: To compare the utility of honey with that of MM as a transport medium for skin biopsy specimens used for DIF and antigen mapping. Methods: Group I consisted of 45 freshly-taken skin specimens earmarked for DIF testing. It was divided into three groups (A, B and C), each containing 15 specimens. Biopsy specimens were sliced into two, one each for MM and honey. Samples in group A were processed at the end of week 1 while those in group B and C were processed at the end of weeks 2 and 4, respectively. Group II consisted of five specimens of epidermolysis bullosa (EB) which was further divided into three groups; two specimens were processed for antigen mapping at the end of week 1, while others were processed at the end of week 2 (two specimens) and 4 (one specimen). Results: Sensitivity of honey as a transport medium for skin biopsy specimens was 100%, 92.6% and 53.8% at weeks 1, 2 and 4, respectively. The antigen mapping was positive in all specimens. Conclusion: Utility of honey was comparable to MM for DIF samples tested at weeks 1 and 2 but was lower at week 4.

AB - Background: Michel's medium (MM) is currently the recommended transport medium for skin biopsy specimens prior to direct immunofluorescence (DIF) microscopy. Objective: To compare the utility of honey with that of MM as a transport medium for skin biopsy specimens used for DIF and antigen mapping. Methods: Group I consisted of 45 freshly-taken skin specimens earmarked for DIF testing. It was divided into three groups (A, B and C), each containing 15 specimens. Biopsy specimens were sliced into two, one each for MM and honey. Samples in group A were processed at the end of week 1 while those in group B and C were processed at the end of weeks 2 and 4, respectively. Group II consisted of five specimens of epidermolysis bullosa (EB) which was further divided into three groups; two specimens were processed for antigen mapping at the end of week 1, while others were processed at the end of week 2 (two specimens) and 4 (one specimen). Results: Sensitivity of honey as a transport medium for skin biopsy specimens was 100%, 92.6% and 53.8% at weeks 1, 2 and 4, respectively. The antigen mapping was positive in all specimens. Conclusion: Utility of honey was comparable to MM for DIF samples tested at weeks 1 and 2 but was lower at week 4.

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