A comparative study to evaluate the effect of limited access dressing (LAD) on burn wound healing

Thittamaranahalli M. Honnegowda, Pramod Kumar, Echalasara G. Padmanabha Udupa, Anurag Sharan, Rekha Singh, Hemanth K. Prasad, Pragna Rao

Research output: Contribution to journalArticle

Abstract

Biochemical and histopathological analyses are commonly used objective parameters in research and clinical fields to assess the healing status of burn wounds. In this study, the effect of newer intermittent negative-pressure wound therapy in combination with moist environment [limited access dressing (LAD)] on burn wound healing is studied. Various biochemical parameters like hydroxyproline, hexosamine and total protein, and antioxidants like reduced glutathione (GSH), glutathione peroxidase (GPx) and oxidative biomarker malondialdhyde (MDA) were measured in the granulation tissue. Histopathologically, necrotic tissue, amount of inflammatory infiltrate, angiogenesis and extracellular matrix deposition (ECM) were studied to determine wound healing. A total of 55 patients were divided into two groups as follows: LAD group (n=28) and conventional dressing group (n=27). Patients treated with LAD have shown significant increase in the mean levels of (±SD) hydroxyproline (75·2±26·30 versus 27·8±15·5; P=0·010), hexosamine (9·0±1·99 versus 8·0±1·18; P=0·038), total protein (15·6±8·23 versus 10·26±4·94; P=0·003), GSH (7·40±1·91 versus 5·1±1·28; P=0·037), GPx (112·6±46·4 versus 92±32·4; P=0·016), and decrease in MDA (6·5±2·24 versus 1 0·6±3·8; P=0·002). Histopathologically, between LAD and conventional dressing groups, there was a significant difference after 10days of treatment (mean±SE) in necrotic tissue of (LAD versus conventional dressing groups=10±1·8 versus 11·9±2·6; P=0·033), inflammatory cells (8·4±1·9 versus 13±3·46; P=0·021), new blood vessels (12·5±2·87 versus 9·4±1·7; P=0·047), ECM deposit (12·9±2·41 versus 9·68±1·3; P=0·018) and showed comparatively fewer inflammatory cells, increased and well-organised extracellular matrix deposit, more angiogenesis in LAD group as compared with that in conventional dressing group. To conclude, LAD exerts its beneficial effects on wound healing by reducing oxidative stress, decreasing necrotic tissue and amount of inflammatory infiltrate, and increasing ECM deposition and angiogenesis.

Original languageEnglish
JournalInternational Wound Journal
DOIs
Publication statusAccepted/In press - 2015

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Bandages
Wound Healing
Extracellular Matrix
Hexosamines
Hydroxyproline
Glutathione Peroxidase
Negative-Pressure Wound Therapy
Granulation Tissue
Glutathione
Blood Vessels
Proteins
Oxidative Stress
Antioxidants
Biomarkers

All Science Journal Classification (ASJC) codes

  • Surgery
  • Dermatology

Cite this

Honnegowda, Thittamaranahalli M. ; Kumar, Pramod ; Padmanabha Udupa, Echalasara G. ; Sharan, Anurag ; Singh, Rekha ; Prasad, Hemanth K. ; Rao, Pragna. / A comparative study to evaluate the effect of limited access dressing (LAD) on burn wound healing. In: International Wound Journal. 2015.
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title = "A comparative study to evaluate the effect of limited access dressing (LAD) on burn wound healing",
abstract = "Biochemical and histopathological analyses are commonly used objective parameters in research and clinical fields to assess the healing status of burn wounds. In this study, the effect of newer intermittent negative-pressure wound therapy in combination with moist environment [limited access dressing (LAD)] on burn wound healing is studied. Various biochemical parameters like hydroxyproline, hexosamine and total protein, and antioxidants like reduced glutathione (GSH), glutathione peroxidase (GPx) and oxidative biomarker malondialdhyde (MDA) were measured in the granulation tissue. Histopathologically, necrotic tissue, amount of inflammatory infiltrate, angiogenesis and extracellular matrix deposition (ECM) were studied to determine wound healing. A total of 55 patients were divided into two groups as follows: LAD group (n=28) and conventional dressing group (n=27). Patients treated with LAD have shown significant increase in the mean levels of (±SD) hydroxyproline (75·2±26·30 versus 27·8±15·5; P=0·010), hexosamine (9·0±1·99 versus 8·0±1·18; P=0·038), total protein (15·6±8·23 versus 10·26±4·94; P=0·003), GSH (7·40±1·91 versus 5·1±1·28; P=0·037), GPx (112·6±46·4 versus 92±32·4; P=0·016), and decrease in MDA (6·5±2·24 versus 1 0·6±3·8; P=0·002). Histopathologically, between LAD and conventional dressing groups, there was a significant difference after 10days of treatment (mean±SE) in necrotic tissue of (LAD versus conventional dressing groups=10±1·8 versus 11·9±2·6; P=0·033), inflammatory cells (8·4±1·9 versus 13±3·46; P=0·021), new blood vessels (12·5±2·87 versus 9·4±1·7; P=0·047), ECM deposit (12·9±2·41 versus 9·68±1·3; P=0·018) and showed comparatively fewer inflammatory cells, increased and well-organised extracellular matrix deposit, more angiogenesis in LAD group as compared with that in conventional dressing group. To conclude, LAD exerts its beneficial effects on wound healing by reducing oxidative stress, decreasing necrotic tissue and amount of inflammatory infiltrate, and increasing ECM deposition and angiogenesis.",
author = "Honnegowda, {Thittamaranahalli M.} and Pramod Kumar and {Padmanabha Udupa}, {Echalasara G.} and Anurag Sharan and Rekha Singh and Prasad, {Hemanth K.} and Pragna Rao",
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A comparative study to evaluate the effect of limited access dressing (LAD) on burn wound healing. / Honnegowda, Thittamaranahalli M.; Kumar, Pramod; Padmanabha Udupa, Echalasara G.; Sharan, Anurag; Singh, Rekha; Prasad, Hemanth K.; Rao, Pragna.

In: International Wound Journal, 2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A comparative study to evaluate the effect of limited access dressing (LAD) on burn wound healing

AU - Honnegowda, Thittamaranahalli M.

AU - Kumar, Pramod

AU - Padmanabha Udupa, Echalasara G.

AU - Sharan, Anurag

AU - Singh, Rekha

AU - Prasad, Hemanth K.

AU - Rao, Pragna

PY - 2015

Y1 - 2015

N2 - Biochemical and histopathological analyses are commonly used objective parameters in research and clinical fields to assess the healing status of burn wounds. In this study, the effect of newer intermittent negative-pressure wound therapy in combination with moist environment [limited access dressing (LAD)] on burn wound healing is studied. Various biochemical parameters like hydroxyproline, hexosamine and total protein, and antioxidants like reduced glutathione (GSH), glutathione peroxidase (GPx) and oxidative biomarker malondialdhyde (MDA) were measured in the granulation tissue. Histopathologically, necrotic tissue, amount of inflammatory infiltrate, angiogenesis and extracellular matrix deposition (ECM) were studied to determine wound healing. A total of 55 patients were divided into two groups as follows: LAD group (n=28) and conventional dressing group (n=27). Patients treated with LAD have shown significant increase in the mean levels of (±SD) hydroxyproline (75·2±26·30 versus 27·8±15·5; P=0·010), hexosamine (9·0±1·99 versus 8·0±1·18; P=0·038), total protein (15·6±8·23 versus 10·26±4·94; P=0·003), GSH (7·40±1·91 versus 5·1±1·28; P=0·037), GPx (112·6±46·4 versus 92±32·4; P=0·016), and decrease in MDA (6·5±2·24 versus 1 0·6±3·8; P=0·002). Histopathologically, between LAD and conventional dressing groups, there was a significant difference after 10days of treatment (mean±SE) in necrotic tissue of (LAD versus conventional dressing groups=10±1·8 versus 11·9±2·6; P=0·033), inflammatory cells (8·4±1·9 versus 13±3·46; P=0·021), new blood vessels (12·5±2·87 versus 9·4±1·7; P=0·047), ECM deposit (12·9±2·41 versus 9·68±1·3; P=0·018) and showed comparatively fewer inflammatory cells, increased and well-organised extracellular matrix deposit, more angiogenesis in LAD group as compared with that in conventional dressing group. To conclude, LAD exerts its beneficial effects on wound healing by reducing oxidative stress, decreasing necrotic tissue and amount of inflammatory infiltrate, and increasing ECM deposition and angiogenesis.

AB - Biochemical and histopathological analyses are commonly used objective parameters in research and clinical fields to assess the healing status of burn wounds. In this study, the effect of newer intermittent negative-pressure wound therapy in combination with moist environment [limited access dressing (LAD)] on burn wound healing is studied. Various biochemical parameters like hydroxyproline, hexosamine and total protein, and antioxidants like reduced glutathione (GSH), glutathione peroxidase (GPx) and oxidative biomarker malondialdhyde (MDA) were measured in the granulation tissue. Histopathologically, necrotic tissue, amount of inflammatory infiltrate, angiogenesis and extracellular matrix deposition (ECM) were studied to determine wound healing. A total of 55 patients were divided into two groups as follows: LAD group (n=28) and conventional dressing group (n=27). Patients treated with LAD have shown significant increase in the mean levels of (±SD) hydroxyproline (75·2±26·30 versus 27·8±15·5; P=0·010), hexosamine (9·0±1·99 versus 8·0±1·18; P=0·038), total protein (15·6±8·23 versus 10·26±4·94; P=0·003), GSH (7·40±1·91 versus 5·1±1·28; P=0·037), GPx (112·6±46·4 versus 92±32·4; P=0·016), and decrease in MDA (6·5±2·24 versus 1 0·6±3·8; P=0·002). Histopathologically, between LAD and conventional dressing groups, there was a significant difference after 10days of treatment (mean±SE) in necrotic tissue of (LAD versus conventional dressing groups=10±1·8 versus 11·9±2·6; P=0·033), inflammatory cells (8·4±1·9 versus 13±3·46; P=0·021), new blood vessels (12·5±2·87 versus 9·4±1·7; P=0·047), ECM deposit (12·9±2·41 versus 9·68±1·3; P=0·018) and showed comparatively fewer inflammatory cells, increased and well-organised extracellular matrix deposit, more angiogenesis in LAD group as compared with that in conventional dressing group. To conclude, LAD exerts its beneficial effects on wound healing by reducing oxidative stress, decreasing necrotic tissue and amount of inflammatory infiltrate, and increasing ECM deposition and angiogenesis.

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