A constitutive catabolite repression mutant of a recombinant Saccharomyces cerevisiae strain improves xylose consumption during fermentation

Vasudevan Thanvanthri Gururajan, Marie F. Gorwa-Grauslund, Bärbel Hahn-Hägerdal, Isak S. Pretorius, Ricardo R. Cordero Otero

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Efficient xylose utilisation by microorganisms is of importance to the lignocellulose fermentation industry. The aim of this work was to develop constitutive catabolite repression mutants in a xylose-utilising recombinant Saccharomyces cerevisiae strain and evaluate the differences in xylose consumption under fermentation conditions. S. cerevisiae YUSM was constitutively catabolite repressed through specific disruptions within the MIG1 gene. The strains were grown aerobically in synthetic complete medium with xylose as the sole carbon source. Constitutive catabolite repressed strain YCR17 grew four-fold better on xylose in aerobic conditions than the control strain YUSM. Anaerobic batch fermentation in minimal medium with glucose-xylose mixtures and N-limited chemostats with varying sugar concentrations were performed. Sugar utilisation and metabolite production during fermentation were monitored. YCR17 exhibited a faster xylose consumption rate than YUSM under high glucose conditions in nitrogen-limited chemostat cultivations. This study shows that a constitutive catabolite repressed mutant could be used to enhance the xylose consumption rate even in the presence of high glucose in the fermentation medium. This could help in reducing fermentation time and cost in mixed sugar fermentation.

Original languageEnglish
Pages (from-to)85-92
Number of pages8
JournalAnnals of Microbiology
Volume57
Issue number1
Publication statusPublished - 2007

Fingerprint

Catabolite Repression
Xylose
Fermentation
Saccharomyces cerevisiae
Glucose
Industry
Nitrogen
Carbon
Costs and Cost Analysis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Microbiology

Cite this

Thanvanthri Gururajan, Vasudevan ; Gorwa-Grauslund, Marie F. ; Hahn-Hägerdal, Bärbel ; Pretorius, Isak S. ; Cordero Otero, Ricardo R. / A constitutive catabolite repression mutant of a recombinant Saccharomyces cerevisiae strain improves xylose consumption during fermentation. In: Annals of Microbiology. 2007 ; Vol. 57, No. 1. pp. 85-92.
@article{6827d1e2c3ea4f3b9b5d34aa5a84db0c,
title = "A constitutive catabolite repression mutant of a recombinant Saccharomyces cerevisiae strain improves xylose consumption during fermentation",
abstract = "Efficient xylose utilisation by microorganisms is of importance to the lignocellulose fermentation industry. The aim of this work was to develop constitutive catabolite repression mutants in a xylose-utilising recombinant Saccharomyces cerevisiae strain and evaluate the differences in xylose consumption under fermentation conditions. S. cerevisiae YUSM was constitutively catabolite repressed through specific disruptions within the MIG1 gene. The strains were grown aerobically in synthetic complete medium with xylose as the sole carbon source. Constitutive catabolite repressed strain YCR17 grew four-fold better on xylose in aerobic conditions than the control strain YUSM. Anaerobic batch fermentation in minimal medium with glucose-xylose mixtures and N-limited chemostats with varying sugar concentrations were performed. Sugar utilisation and metabolite production during fermentation were monitored. YCR17 exhibited a faster xylose consumption rate than YUSM under high glucose conditions in nitrogen-limited chemostat cultivations. This study shows that a constitutive catabolite repressed mutant could be used to enhance the xylose consumption rate even in the presence of high glucose in the fermentation medium. This could help in reducing fermentation time and cost in mixed sugar fermentation.",
author = "{Thanvanthri Gururajan}, Vasudevan and Gorwa-Grauslund, {Marie F.} and B{\"a}rbel Hahn-H{\"a}gerdal and Pretorius, {Isak S.} and {Cordero Otero}, {Ricardo R.}",
year = "2007",
language = "English",
volume = "57",
pages = "85--92",
journal = "Annals of Microbiology",
issn = "1590-4261",
publisher = "Springer Heidelberg",
number = "1",

}

A constitutive catabolite repression mutant of a recombinant Saccharomyces cerevisiae strain improves xylose consumption during fermentation. / Thanvanthri Gururajan, Vasudevan; Gorwa-Grauslund, Marie F.; Hahn-Hägerdal, Bärbel; Pretorius, Isak S.; Cordero Otero, Ricardo R.

In: Annals of Microbiology, Vol. 57, No. 1, 2007, p. 85-92.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A constitutive catabolite repression mutant of a recombinant Saccharomyces cerevisiae strain improves xylose consumption during fermentation

AU - Thanvanthri Gururajan, Vasudevan

AU - Gorwa-Grauslund, Marie F.

AU - Hahn-Hägerdal, Bärbel

AU - Pretorius, Isak S.

AU - Cordero Otero, Ricardo R.

PY - 2007

Y1 - 2007

N2 - Efficient xylose utilisation by microorganisms is of importance to the lignocellulose fermentation industry. The aim of this work was to develop constitutive catabolite repression mutants in a xylose-utilising recombinant Saccharomyces cerevisiae strain and evaluate the differences in xylose consumption under fermentation conditions. S. cerevisiae YUSM was constitutively catabolite repressed through specific disruptions within the MIG1 gene. The strains were grown aerobically in synthetic complete medium with xylose as the sole carbon source. Constitutive catabolite repressed strain YCR17 grew four-fold better on xylose in aerobic conditions than the control strain YUSM. Anaerobic batch fermentation in minimal medium with glucose-xylose mixtures and N-limited chemostats with varying sugar concentrations were performed. Sugar utilisation and metabolite production during fermentation were monitored. YCR17 exhibited a faster xylose consumption rate than YUSM under high glucose conditions in nitrogen-limited chemostat cultivations. This study shows that a constitutive catabolite repressed mutant could be used to enhance the xylose consumption rate even in the presence of high glucose in the fermentation medium. This could help in reducing fermentation time and cost in mixed sugar fermentation.

AB - Efficient xylose utilisation by microorganisms is of importance to the lignocellulose fermentation industry. The aim of this work was to develop constitutive catabolite repression mutants in a xylose-utilising recombinant Saccharomyces cerevisiae strain and evaluate the differences in xylose consumption under fermentation conditions. S. cerevisiae YUSM was constitutively catabolite repressed through specific disruptions within the MIG1 gene. The strains were grown aerobically in synthetic complete medium with xylose as the sole carbon source. Constitutive catabolite repressed strain YCR17 grew four-fold better on xylose in aerobic conditions than the control strain YUSM. Anaerobic batch fermentation in minimal medium with glucose-xylose mixtures and N-limited chemostats with varying sugar concentrations were performed. Sugar utilisation and metabolite production during fermentation were monitored. YCR17 exhibited a faster xylose consumption rate than YUSM under high glucose conditions in nitrogen-limited chemostat cultivations. This study shows that a constitutive catabolite repressed mutant could be used to enhance the xylose consumption rate even in the presence of high glucose in the fermentation medium. This could help in reducing fermentation time and cost in mixed sugar fermentation.

UR - http://www.scopus.com/inward/record.url?scp=34147110457&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34147110457&partnerID=8YFLogxK

M3 - Article

VL - 57

SP - 85

EP - 92

JO - Annals of Microbiology

JF - Annals of Microbiology

SN - 1590-4261

IS - 1

ER -