A laser Raman tweezers study of eryptosis

Surekha Barkur, Deepak Mathur, Santhosh Chidangil

Research output: Contribution to journalArticle

Abstract

Eryptosis—the suicidal death of erythrocytes—is characterized by membrane blebbing and cell shrinkage. Eryptosis can be triggered by various xenobiotics such as carbon monoxide, lead, and amyloid, and by stressors such as oxidative stress, osmotic shock, and rapid alteration of ambient conditions. We have used Raman tweezers spectroscopy to study eryptosis in single, live cells and have attempted to explore the underlying mechanism, specifically to identify possible Raman signatures of eryptosis. Erythrocytes (red blood cells) were exposed to free radicals, silver nanoparticles, glucose, heat, and osmotic shock to induce eryptosis, and a comparison was made of their Raman spectra, which indicated that these conditions lead to a transition of haemoglobin from the R to the T state. Consequences of eryptosis include dehydration, cell shrinkage, and pH changes, which result in deoxygenation of haemoglobin. This, in turn, can be detected by monitoring the wavenumber shifts associated with Raman marker bands of R to T transitions. In addition, the principal component analysis results indicate differentiation among red blood cells undergone eryptosis due to different conditions.

LanguageEnglish
Pages1155-1164
Number of pages10
JournalJournal of Raman Spectroscopy
Volume49
Issue number7
DOIs
Publication statusPublished - 01-07-2018

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Hemoglobin
Hemoglobins
Blood
Cells
Oxidative stress
Lasers
Xenobiotics
Carbon Monoxide
Free radicals
Dehydration
Silver
Amyloid
Carbon monoxide
Principal component analysis
Free Radicals
Glucose
Raman scattering
Lead
Spectroscopy
Nanoparticles

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Spectroscopy

Cite this

Barkur, Surekha ; Mathur, Deepak ; Chidangil, Santhosh. / A laser Raman tweezers study of eryptosis. In: Journal of Raman Spectroscopy. 2018 ; Vol. 49, No. 7. pp. 1155-1164.
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A laser Raman tweezers study of eryptosis. / Barkur, Surekha; Mathur, Deepak; Chidangil, Santhosh.

In: Journal of Raman Spectroscopy, Vol. 49, No. 7, 01.07.2018, p. 1155-1164.

Research output: Contribution to journalArticle

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