A method to score micronuclei in vivo using cytochalasin B-induced cytokinesis block

P. Uma Devi, B. S. Satish Rao, Ravindra Kamath

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The present paper describes an in vivo micronucleus assay using Cytochalasin B (CyB). Mice bearing three different tumours, fibrosarcoma (Swiss albino mice), B16 F1 melanoma (C57 BL) and Ehrlich ascites carcinoma (Swiss albino mice), were injected with repeated doses of CyB at different time intervals and binucleate cells were scored at 24, 36, 48, 60 and 72 h after CyB injection. It was found that three doses of 3 + 2 + 2 mg/kg CyB administered intraperitoneally (i.p.) at 12-h intervals effectively blocked cytokinesis. The maximum number of binucleated cells (BNC) was scored at 60 h after the last CyB dose. This dose schedule was also effective in scoring micronuclei in BNC after irradiation.

Original languageEnglish
Pages (from-to)33-37
Number of pages5
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume401
Issue number1-2
DOIs
Publication statusPublished - 05-06-1998

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Cytochalasin B
Cytokinesis
Micronucleus Tests
Experimental Melanomas
Fibrosarcoma
Ascites
Appointments and Schedules
Cell Count
Carcinoma
Injections
Neoplasms

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

Cite this

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abstract = "The present paper describes an in vivo micronucleus assay using Cytochalasin B (CyB). Mice bearing three different tumours, fibrosarcoma (Swiss albino mice), B16 F1 melanoma (C57 BL) and Ehrlich ascites carcinoma (Swiss albino mice), were injected with repeated doses of CyB at different time intervals and binucleate cells were scored at 24, 36, 48, 60 and 72 h after CyB injection. It was found that three doses of 3 + 2 + 2 mg/kg CyB administered intraperitoneally (i.p.) at 12-h intervals effectively blocked cytokinesis. The maximum number of binucleated cells (BNC) was scored at 60 h after the last CyB dose. This dose schedule was also effective in scoring micronuclei in BNC after irradiation.",
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A method to score micronuclei in vivo using cytochalasin B-induced cytokinesis block. / Uma Devi, P.; Satish Rao, B. S.; Kamath, Ravindra.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 401, No. 1-2, 05.06.1998, p. 33-37.

Research output: Contribution to journalArticle

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