A modified spectrophotometric micromethod to determine serum copper

Mungli Prakash, Jeevan K. Shetty

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Most of the available spectrophotometric methods to determine serum copper requires large volume of sample and involves several steps. We modified literature known method to estimate serum copper. Guanidine hydrocloride was used to release ceruloplasmin bound copper and determined using bathocuproine disulphonate disodium salt (BCDS). Our modified micromethod correlated well with the literature known method and shown good recovery and precision. Modified spectrophotometric micromethod is simple, sensitive, rapid, requires very less sample and coloring reagent and can be adopted in any clinical laboratory setups.

Original languageEnglish
Pages (from-to)38-42
Number of pages5
JournalAsian Journal of Biochemistry
Volume3
Issue number1
DOIs
Publication statusPublished - 01-01-2008
Externally publishedYes

Fingerprint

Copper
Serum
Clinical laboratories
Ceruloplasmin
Guanidine
Coloring
Salts
Recovery
bathocuproine

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biochemistry, medical

Cite this

Prakash, Mungli ; Shetty, Jeevan K. / A modified spectrophotometric micromethod to determine serum copper. In: Asian Journal of Biochemistry. 2008 ; Vol. 3, No. 1. pp. 38-42.
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A modified spectrophotometric micromethod to determine serum copper. / Prakash, Mungli; Shetty, Jeevan K.

In: Asian Journal of Biochemistry, Vol. 3, No. 1, 01.01.2008, p. 38-42.

Research output: Contribution to journalArticle

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