A multicenter prospective hospital-based surveillance to estimate the burden of rotavirus gastroenteritis in children less than five years of age in India

T. Saluja, S. D. Sharma, M. Gupta, R. Kundu, S. Kar, A. Dutta, M. Silveira, J. V. Singh, V. G. Kamath, A. Chaudhary, J. V. Rao, M. D. Ravi, S. R.K. Murthy, S. Babji, R. Prasad, R. Gujjula, R. Rao, M. S. Dhingra

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Rotavirus is the leading cause of severe, dehydrating diarrhea in children aged <5 years globally, with an estimated 25 million outpatient visits and 2 million hospitalizations attributable to rotavirus infections each year. The aim of this hospital-based surveillance was to summarize the local epidemiological and virological features of rotavirus and to estimate the disease burden in the population under surveillance in India. Methods: During the 16 months surveillance period from April 2011 through July 2012, a total of 4711 children under the age of 5 years were admitted with acute diarrhea at 12 medical centers attached to medical schools throughout India. Stool samples were randomly collected from 2051 (43.5%) subjects and were analyzed for rotavirus positivity using commercial enzyme immunoassay kit (Premier Rotaclone Qualitative Elisa) at the respective study centers. Rotavirus positive samples were genotyped for VP7 and VP4 by reverse-transcription polymerase chain reaction (RT-PCR) at a central laboratory. Results: During the study period, maximum number of rotavirus related hospitalizations were reported from December 2011 through February 2012. Out of the 2051 stool samples tested for rotavirus, overall 541 (26.4%) samples were positive for rotavirus VP6 antigen in stool. The highest positivity was observed in the month of December, 2011 (52.5%) and lowest in the month of May, 2011 (10.3%). We found that majority of the rotavirus positive cases (69.7%) were in children <24 months of age. The most common genotypes reported were G1 (38%), G2 (18%), G9 (18%), G12 (9%) and mixed strains (17%). Conclusions: The results of this study confirm the significant burden of acute rotavirus gastroenteritis as a cause of hospitalizations in under five children in India.

Original languageEnglish
JournalVaccine
Volume32
Issue numberS1
DOIs
Publication statusPublished - 01-01-2014

Fingerprint

Rotavirus
gastroenteritis
Gastroenteritis
India
monitoring
Hospitalization
Diarrhea
diarrhea
Population Surveillance
Rotavirus Infections
sampling
burden of disease
Medical Schools
enzyme immunoassays
Immunoenzyme Techniques
Reverse Transcription
Outpatients
Genotype
reverse transcriptase polymerase chain reaction
Antigens

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Saluja, T. ; Sharma, S. D. ; Gupta, M. ; Kundu, R. ; Kar, S. ; Dutta, A. ; Silveira, M. ; Singh, J. V. ; Kamath, V. G. ; Chaudhary, A. ; Rao, J. V. ; Ravi, M. D. ; Murthy, S. R.K. ; Babji, S. ; Prasad, R. ; Gujjula, R. ; Rao, R. ; Dhingra, M. S. / A multicenter prospective hospital-based surveillance to estimate the burden of rotavirus gastroenteritis in children less than five years of age in India. In: Vaccine. 2014 ; Vol. 32, No. S1.
@article{a1391184df694d76907087c5b574fe6b,
title = "A multicenter prospective hospital-based surveillance to estimate the burden of rotavirus gastroenteritis in children less than five years of age in India",
abstract = "Background: Rotavirus is the leading cause of severe, dehydrating diarrhea in children aged <5 years globally, with an estimated 25 million outpatient visits and 2 million hospitalizations attributable to rotavirus infections each year. The aim of this hospital-based surveillance was to summarize the local epidemiological and virological features of rotavirus and to estimate the disease burden in the population under surveillance in India. Methods: During the 16 months surveillance period from April 2011 through July 2012, a total of 4711 children under the age of 5 years were admitted with acute diarrhea at 12 medical centers attached to medical schools throughout India. Stool samples were randomly collected from 2051 (43.5{\%}) subjects and were analyzed for rotavirus positivity using commercial enzyme immunoassay kit (Premier Rotaclone Qualitative Elisa) at the respective study centers. Rotavirus positive samples were genotyped for VP7 and VP4 by reverse-transcription polymerase chain reaction (RT-PCR) at a central laboratory. Results: During the study period, maximum number of rotavirus related hospitalizations were reported from December 2011 through February 2012. Out of the 2051 stool samples tested for rotavirus, overall 541 (26.4{\%}) samples were positive for rotavirus VP6 antigen in stool. The highest positivity was observed in the month of December, 2011 (52.5{\%}) and lowest in the month of May, 2011 (10.3{\%}). We found that majority of the rotavirus positive cases (69.7{\%}) were in children <24 months of age. The most common genotypes reported were G1 (38{\%}), G2 (18{\%}), G9 (18{\%}), G12 (9{\%}) and mixed strains (17{\%}). Conclusions: The results of this study confirm the significant burden of acute rotavirus gastroenteritis as a cause of hospitalizations in under five children in India.",
author = "T. Saluja and Sharma, {S. D.} and M. Gupta and R. Kundu and S. Kar and A. Dutta and M. Silveira and Singh, {J. V.} and Kamath, {V. G.} and A. Chaudhary and Rao, {J. V.} and Ravi, {M. D.} and Murthy, {S. R.K.} and S. Babji and R. Prasad and R. Gujjula and R. Rao and Dhingra, {M. S.}",
year = "2014",
month = "1",
day = "1",
doi = "10.1016/j.vaccine.2014.03.030",
language = "English",
volume = "32",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "S1",

}

Saluja, T, Sharma, SD, Gupta, M, Kundu, R, Kar, S, Dutta, A, Silveira, M, Singh, JV, Kamath, VG, Chaudhary, A, Rao, JV, Ravi, MD, Murthy, SRK, Babji, S, Prasad, R, Gujjula, R, Rao, R & Dhingra, MS 2014, 'A multicenter prospective hospital-based surveillance to estimate the burden of rotavirus gastroenteritis in children less than five years of age in India', Vaccine, vol. 32, no. S1. https://doi.org/10.1016/j.vaccine.2014.03.030

A multicenter prospective hospital-based surveillance to estimate the burden of rotavirus gastroenteritis in children less than five years of age in India. / Saluja, T.; Sharma, S. D.; Gupta, M.; Kundu, R.; Kar, S.; Dutta, A.; Silveira, M.; Singh, J. V.; Kamath, V. G.; Chaudhary, A.; Rao, J. V.; Ravi, M. D.; Murthy, S. R.K.; Babji, S.; Prasad, R.; Gujjula, R.; Rao, R.; Dhingra, M. S.

In: Vaccine, Vol. 32, No. S1, 01.01.2014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A multicenter prospective hospital-based surveillance to estimate the burden of rotavirus gastroenteritis in children less than five years of age in India

AU - Saluja, T.

AU - Sharma, S. D.

AU - Gupta, M.

AU - Kundu, R.

AU - Kar, S.

AU - Dutta, A.

AU - Silveira, M.

AU - Singh, J. V.

AU - Kamath, V. G.

AU - Chaudhary, A.

AU - Rao, J. V.

AU - Ravi, M. D.

AU - Murthy, S. R.K.

AU - Babji, S.

AU - Prasad, R.

AU - Gujjula, R.

AU - Rao, R.

AU - Dhingra, M. S.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background: Rotavirus is the leading cause of severe, dehydrating diarrhea in children aged <5 years globally, with an estimated 25 million outpatient visits and 2 million hospitalizations attributable to rotavirus infections each year. The aim of this hospital-based surveillance was to summarize the local epidemiological and virological features of rotavirus and to estimate the disease burden in the population under surveillance in India. Methods: During the 16 months surveillance period from April 2011 through July 2012, a total of 4711 children under the age of 5 years were admitted with acute diarrhea at 12 medical centers attached to medical schools throughout India. Stool samples were randomly collected from 2051 (43.5%) subjects and were analyzed for rotavirus positivity using commercial enzyme immunoassay kit (Premier Rotaclone Qualitative Elisa) at the respective study centers. Rotavirus positive samples were genotyped for VP7 and VP4 by reverse-transcription polymerase chain reaction (RT-PCR) at a central laboratory. Results: During the study period, maximum number of rotavirus related hospitalizations were reported from December 2011 through February 2012. Out of the 2051 stool samples tested for rotavirus, overall 541 (26.4%) samples were positive for rotavirus VP6 antigen in stool. The highest positivity was observed in the month of December, 2011 (52.5%) and lowest in the month of May, 2011 (10.3%). We found that majority of the rotavirus positive cases (69.7%) were in children <24 months of age. The most common genotypes reported were G1 (38%), G2 (18%), G9 (18%), G12 (9%) and mixed strains (17%). Conclusions: The results of this study confirm the significant burden of acute rotavirus gastroenteritis as a cause of hospitalizations in under five children in India.

AB - Background: Rotavirus is the leading cause of severe, dehydrating diarrhea in children aged <5 years globally, with an estimated 25 million outpatient visits and 2 million hospitalizations attributable to rotavirus infections each year. The aim of this hospital-based surveillance was to summarize the local epidemiological and virological features of rotavirus and to estimate the disease burden in the population under surveillance in India. Methods: During the 16 months surveillance period from April 2011 through July 2012, a total of 4711 children under the age of 5 years were admitted with acute diarrhea at 12 medical centers attached to medical schools throughout India. Stool samples were randomly collected from 2051 (43.5%) subjects and were analyzed for rotavirus positivity using commercial enzyme immunoassay kit (Premier Rotaclone Qualitative Elisa) at the respective study centers. Rotavirus positive samples were genotyped for VP7 and VP4 by reverse-transcription polymerase chain reaction (RT-PCR) at a central laboratory. Results: During the study period, maximum number of rotavirus related hospitalizations were reported from December 2011 through February 2012. Out of the 2051 stool samples tested for rotavirus, overall 541 (26.4%) samples were positive for rotavirus VP6 antigen in stool. The highest positivity was observed in the month of December, 2011 (52.5%) and lowest in the month of May, 2011 (10.3%). We found that majority of the rotavirus positive cases (69.7%) were in children <24 months of age. The most common genotypes reported were G1 (38%), G2 (18%), G9 (18%), G12 (9%) and mixed strains (17%). Conclusions: The results of this study confirm the significant burden of acute rotavirus gastroenteritis as a cause of hospitalizations in under five children in India.

UR - http://www.scopus.com/inward/record.url?scp=84905190711&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84905190711&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2014.03.030

DO - 10.1016/j.vaccine.2014.03.030

M3 - Article

VL - 32

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - S1

ER -