A novel mutation (c.121-13T>A) in the polypyrimidine tract of the splice acceptor site of intron 2 causes exon 3 skipping in Mitochondrial acetoacetyl-CoA thiolase gene

Yuka Aoyama, Hideo Sasai, Elsayed Abdelkreem, Hiroki Otsuka, Mina Nakama, Sandeep Kumar, Shrikiran Aroor, Anju Shukla, Toshiyuki Fukao

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mitochondrial acetoacetyl-CoA thiolase (T2) (gene symbol: ACAT1) deficiency is an autosomal recessive disorder affecting isoleucine catabolism and ketone body utilization. In this study, mutational analysis of an Indian T2-deficient patient revealed a homozygous mutation (c.121-13T>A) located at the polypyrimidine tract of the splice acceptor site of intron 2, and exon 3 skipping was identified by cDNA analysis using cycloheximide. We made three mutant constructs (c.121-13T>A, T>C, and T>G substitutions) followed by making a wild-type minigene construct that included an ACAT1 segment from exon 2 to 4 for a splicing experiment. The minigene splicing experiment demonstrated that exon 3 skipping was induced not only by c.121-13T>A mutation, but also by the other two substitutions. It was difficult to predict the effect of these mutations on splicing using in silico tools, as predictions of different tools were inconsistent with each other. The minigene splicing experiment remains the most reliable method to unravel splicing abnormalities.

Original languageEnglish
Pages (from-to)3879-3884
Number of pages6
JournalMolecular Medicine Reports
Volume15
Issue number6
DOIs
Publication statusPublished - 01-06-2017

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Acetyl-CoA C-Acetyltransferase
RNA Splice Sites
Introns
Exons
Genes
Mutation
Substitution reactions
Ketone Bodies
Isoleucine
Experiments
Cycloheximide
Computer Simulation
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Oncology
  • Genetics
  • Cancer Research

Cite this

Aoyama, Yuka ; Sasai, Hideo ; Abdelkreem, Elsayed ; Otsuka, Hiroki ; Nakama, Mina ; Kumar, Sandeep ; Aroor, Shrikiran ; Shukla, Anju ; Fukao, Toshiyuki. / A novel mutation (c.121-13T>A) in the polypyrimidine tract of the splice acceptor site of intron 2 causes exon 3 skipping in Mitochondrial acetoacetyl-CoA thiolase gene. In: Molecular Medicine Reports. 2017 ; Vol. 15, No. 6. pp. 3879-3884.
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A novel mutation (c.121-13T>A) in the polypyrimidine tract of the splice acceptor site of intron 2 causes exon 3 skipping in Mitochondrial acetoacetyl-CoA thiolase gene. / Aoyama, Yuka; Sasai, Hideo; Abdelkreem, Elsayed; Otsuka, Hiroki; Nakama, Mina; Kumar, Sandeep; Aroor, Shrikiran; Shukla, Anju; Fukao, Toshiyuki.

In: Molecular Medicine Reports, Vol. 15, No. 6, 01.06.2017, p. 3879-3884.

Research output: Contribution to journalArticle

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AU - Aroor, Shrikiran

AU - Shukla, Anju

AU - Fukao, Toshiyuki

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