A rapid ultra HPLC-MS/MS method for the quantitation and pharmacokinetic analysis of 3-deazaneplanocin A in mice

Cody J. Peer, Mahadev Rao, Shawn D. Spencer, Shandiz Shahbazi, Patricia S. Steeg, David S. Schrump, William D. Figg

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

3-Deazaneplanocin A (DZNep) has been shown to have anti-cancer activity in numerous cancer types and its continued preclinical, and eventual clinical, drug development will require rapid and sensitive bioanalytical methods in order to quantitate this drug for pharmacokinetic analyses. The ultra HPLC with positive thermospray tandem mass spectrometric (LC-MS/MS) detection affords the most sensitive (limit of quantitation 5. ng/mL) and rapid (3. min run time) bioanalytical method to date for DZNep. Due to the polar nature of this drug and the internal standard (tubercidin), a hydrophilic-interaction column (HILIC) was used. The method was accurate, with less than 10% deviation from nominal values, as well as precise, where both within-day and between-day precisions were less than 15%. A liquid-liquid extraction procedure was able to recover ~90% of drug from a small volume (50. μL) of mouse plasma. This method was successfully applied to a pharmacokinetic study in mice intravenously injected with DZNep.

Original languageEnglish
Pages (from-to)142-146
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume927
DOIs
Publication statusPublished - 05-05-2013

Fingerprint

Pharmacokinetics
High Pressure Liquid Chromatography
Pharmaceutical Preparations
Tubercidin
Liquids
Liquid-Liquid Extraction
Hydrophobic and Hydrophilic Interactions
Neoplasms
Plasmas
3-deazaneplanocin

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

Peer, Cody J. ; Rao, Mahadev ; Spencer, Shawn D. ; Shahbazi, Shandiz ; Steeg, Patricia S. ; Schrump, David S. ; Figg, William D. / A rapid ultra HPLC-MS/MS method for the quantitation and pharmacokinetic analysis of 3-deazaneplanocin A in mice. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2013 ; Vol. 927. pp. 142-146.
@article{bee93353280945f484770a3d0270ff9c,
title = "A rapid ultra HPLC-MS/MS method for the quantitation and pharmacokinetic analysis of 3-deazaneplanocin A in mice",
abstract = "3-Deazaneplanocin A (DZNep) has been shown to have anti-cancer activity in numerous cancer types and its continued preclinical, and eventual clinical, drug development will require rapid and sensitive bioanalytical methods in order to quantitate this drug for pharmacokinetic analyses. The ultra HPLC with positive thermospray tandem mass spectrometric (LC-MS/MS) detection affords the most sensitive (limit of quantitation 5. ng/mL) and rapid (3. min run time) bioanalytical method to date for DZNep. Due to the polar nature of this drug and the internal standard (tubercidin), a hydrophilic-interaction column (HILIC) was used. The method was accurate, with less than 10{\%} deviation from nominal values, as well as precise, where both within-day and between-day precisions were less than 15{\%}. A liquid-liquid extraction procedure was able to recover ~90{\%} of drug from a small volume (50. μL) of mouse plasma. This method was successfully applied to a pharmacokinetic study in mice intravenously injected with DZNep.",
author = "Peer, {Cody J.} and Mahadev Rao and Spencer, {Shawn D.} and Shandiz Shahbazi and Steeg, {Patricia S.} and Schrump, {David S.} and Figg, {William D.}",
year = "2013",
month = "5",
day = "5",
doi = "10.1016/j.jchromb.2013.01.003",
language = "English",
volume = "927",
pages = "142--146",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",

}

A rapid ultra HPLC-MS/MS method for the quantitation and pharmacokinetic analysis of 3-deazaneplanocin A in mice. / Peer, Cody J.; Rao, Mahadev; Spencer, Shawn D.; Shahbazi, Shandiz; Steeg, Patricia S.; Schrump, David S.; Figg, William D.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 927, 05.05.2013, p. 142-146.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A rapid ultra HPLC-MS/MS method for the quantitation and pharmacokinetic analysis of 3-deazaneplanocin A in mice

AU - Peer, Cody J.

AU - Rao, Mahadev

AU - Spencer, Shawn D.

AU - Shahbazi, Shandiz

AU - Steeg, Patricia S.

AU - Schrump, David S.

AU - Figg, William D.

PY - 2013/5/5

Y1 - 2013/5/5

N2 - 3-Deazaneplanocin A (DZNep) has been shown to have anti-cancer activity in numerous cancer types and its continued preclinical, and eventual clinical, drug development will require rapid and sensitive bioanalytical methods in order to quantitate this drug for pharmacokinetic analyses. The ultra HPLC with positive thermospray tandem mass spectrometric (LC-MS/MS) detection affords the most sensitive (limit of quantitation 5. ng/mL) and rapid (3. min run time) bioanalytical method to date for DZNep. Due to the polar nature of this drug and the internal standard (tubercidin), a hydrophilic-interaction column (HILIC) was used. The method was accurate, with less than 10% deviation from nominal values, as well as precise, where both within-day and between-day precisions were less than 15%. A liquid-liquid extraction procedure was able to recover ~90% of drug from a small volume (50. μL) of mouse plasma. This method was successfully applied to a pharmacokinetic study in mice intravenously injected with DZNep.

AB - 3-Deazaneplanocin A (DZNep) has been shown to have anti-cancer activity in numerous cancer types and its continued preclinical, and eventual clinical, drug development will require rapid and sensitive bioanalytical methods in order to quantitate this drug for pharmacokinetic analyses. The ultra HPLC with positive thermospray tandem mass spectrometric (LC-MS/MS) detection affords the most sensitive (limit of quantitation 5. ng/mL) and rapid (3. min run time) bioanalytical method to date for DZNep. Due to the polar nature of this drug and the internal standard (tubercidin), a hydrophilic-interaction column (HILIC) was used. The method was accurate, with less than 10% deviation from nominal values, as well as precise, where both within-day and between-day precisions were less than 15%. A liquid-liquid extraction procedure was able to recover ~90% of drug from a small volume (50. μL) of mouse plasma. This method was successfully applied to a pharmacokinetic study in mice intravenously injected with DZNep.

UR - http://www.scopus.com/inward/record.url?scp=84876959022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876959022&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2013.01.003

DO - 10.1016/j.jchromb.2013.01.003

M3 - Article

VL - 927

SP - 142

EP - 146

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

ER -