A rat model against chemotherapy plus radiation-induced oral mucositis

Alkesh Patel, S. Rajesh, V. M. Chandrashekhar, Shivprakash Rathnam, Karishma Shah, C. Mallikarjuna Rao, K. Nandakumar

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objectives: Present study was aimed at developing an experimental model of oral mucositis in rats using a combination of chemotherapeutic agent and radiation. Study design: Female Wistar rats (150-200g) were divided into 3 groups (n=6). Rats in group 1 (normal control) and group 2 (mucositis control) were treated with vehicle. Rats in group 3 were treated with l-glutamine (1g/kg, p.o.; 15days) before and after mucositis induction. Oral mucositis was induced by busulfan (6mg/kg, p.o.; 4days) and the tongue exposed to infrared (IR) radiation of intensity 40mV/cm2 for 5 s on the 1st, 4th and 10th days of challenge using a tail flick apparatus. Parameters monitored were body weight, food intake, blood count and survival. Oral mucositis score (OMS) was recorded daily. Histological changes of the irradiated tongue were assessed by hematoxylin and eosin staining. Results: Busulfan and IR radiation significantly reduced body weight and food intake of the mucositis control group as compared to normal control. Clear ulceration of the tongue reflected in the OMS. Histopathology of the tongue revealed intense lymphocytic infiltration, decreased thickness of squamous epithelial cell layer, decrease in number of blood vessels, and necrosis of cells along with pseudo-membrane formation in the mucositis control group. These findings suggested that oral mucositis was successfully induced and treatment with l-glutamine partially reversed these conditions. Conclusion: Oral mucositis was established successfully in rats by the combination of chemotherapeutic agent and IR radiation. This may be a useful model for screening drugs in the treatment of oral mucositis.

Original languageEnglish
Pages (from-to)399-403
Number of pages5
JournalSaudi Pharmaceutical Journal
Volume21
Issue number4
DOIs
Publication statusPublished - 10-2013

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Stomatitis
Radiation
Mucositis
Drug Therapy
Tongue
Busulfan
Glutamine
Control Groups
Eating
Body Weight
Preclinical Drug Evaluations
Hematoxylin
Eosine Yellowish-(YS)
Blood Vessels
Tail
Wistar Rats
Theoretical Models
Necrosis
Epithelial Cells
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Pharmacology

Cite this

Patel, Alkesh ; Rajesh, S. ; Chandrashekhar, V. M. ; Rathnam, Shivprakash ; Shah, Karishma ; Mallikarjuna Rao, C. ; Nandakumar, K. / A rat model against chemotherapy plus radiation-induced oral mucositis. In: Saudi Pharmaceutical Journal. 2013 ; Vol. 21, No. 4. pp. 399-403.
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abstract = "Objectives: Present study was aimed at developing an experimental model of oral mucositis in rats using a combination of chemotherapeutic agent and radiation. Study design: Female Wistar rats (150-200g) were divided into 3 groups (n=6). Rats in group 1 (normal control) and group 2 (mucositis control) were treated with vehicle. Rats in group 3 were treated with l-glutamine (1g/kg, p.o.; 15days) before and after mucositis induction. Oral mucositis was induced by busulfan (6mg/kg, p.o.; 4days) and the tongue exposed to infrared (IR) radiation of intensity 40mV/cm2 for 5 s on the 1st, 4th and 10th days of challenge using a tail flick apparatus. Parameters monitored were body weight, food intake, blood count and survival. Oral mucositis score (OMS) was recorded daily. Histological changes of the irradiated tongue were assessed by hematoxylin and eosin staining. Results: Busulfan and IR radiation significantly reduced body weight and food intake of the mucositis control group as compared to normal control. Clear ulceration of the tongue reflected in the OMS. Histopathology of the tongue revealed intense lymphocytic infiltration, decreased thickness of squamous epithelial cell layer, decrease in number of blood vessels, and necrosis of cells along with pseudo-membrane formation in the mucositis control group. These findings suggested that oral mucositis was successfully induced and treatment with l-glutamine partially reversed these conditions. Conclusion: Oral mucositis was established successfully in rats by the combination of chemotherapeutic agent and IR radiation. This may be a useful model for screening drugs in the treatment of oral mucositis.",
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A rat model against chemotherapy plus radiation-induced oral mucositis. / Patel, Alkesh; Rajesh, S.; Chandrashekhar, V. M.; Rathnam, Shivprakash; Shah, Karishma; Mallikarjuna Rao, C.; Nandakumar, K.

In: Saudi Pharmaceutical Journal, Vol. 21, No. 4, 10.2013, p. 399-403.

Research output: Contribution to journalArticle

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AU - Patel, Alkesh

AU - Rajesh, S.

AU - Chandrashekhar, V. M.

AU - Rathnam, Shivprakash

AU - Shah, Karishma

AU - Mallikarjuna Rao, C.

AU - Nandakumar, K.

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