A sensitive and selective HPLC method for estimation of lamotrigine in human plasma and saliva: Application to plasma-saliva correlation in epileptic patients

S.R. Mallayasamy, K. Arumugam, T. Jain, T. Rajakannan, K. Bhat, P. Gurumadhavrao, R. Devarakonda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A sensitive and selective high performance liquid chromatographic (HPLC) method was developed and validated for estimation of lamotrigine (CAS 84057-84-1) in human plasma and saliva. The chromatographic separation was achieved with a reversed phase column and a mobile phase consisting of acetonitrile and 20 mM ammoniumacetate buffer pH6.5 (30 : 70) with a flow rate of 1 mL/min. The calibration curve was linear within the working range for both plasma and saliva. The validated method has been successfully applied for a study of lamotrigine in human plasma and saliva to establish the correlation between these two matrices. A scatter plot of plasma versus salivary lamotrigine concentrations showed a good linear relationship between them (Pearson correlation coefficient, r = 0.6832, p <0.001). © ECV · Editio Cantor Verlag.
Original languageEnglish
Pages (from-to)599-606
Number of pages8
JournalArzneimittel-Forschung/Drug Research
Volume60
Issue number10
Publication statusPublished - 2010

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Saliva
Calibration
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lamotrigine

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title = "A sensitive and selective HPLC method for estimation of lamotrigine in human plasma and saliva: Application to plasma-saliva correlation in epileptic patients",
abstract = "A sensitive and selective high performance liquid chromatographic (HPLC) method was developed and validated for estimation of lamotrigine (CAS 84057-84-1) in human plasma and saliva. The chromatographic separation was achieved with a reversed phase column and a mobile phase consisting of acetonitrile and 20 mM ammoniumacetate buffer pH6.5 (30 : 70) with a flow rate of 1 mL/min. The calibration curve was linear within the working range for both plasma and saliva. The validated method has been successfully applied for a study of lamotrigine in human plasma and saliva to establish the correlation between these two matrices. A scatter plot of plasma versus salivary lamotrigine concentrations showed a good linear relationship between them (Pearson correlation coefficient, r = 0.6832, p <0.001). {\circledC} ECV · Editio Cantor Verlag.",
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A sensitive and selective HPLC method for estimation of lamotrigine in human plasma and saliva: Application to plasma-saliva correlation in epileptic patients. / Mallayasamy, S.R.; Arumugam, K.; Jain, T.; Rajakannan, T.; Bhat, K.; Gurumadhavrao, P.; Devarakonda, R.

In: Arzneimittel-Forschung/Drug Research, Vol. 60, No. 10, 2010, p. 599-606.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A sensitive and selective HPLC method for estimation of lamotrigine in human plasma and saliva: Application to plasma-saliva correlation in epileptic patients

AU - Mallayasamy, S.R.

AU - Arumugam, K.

AU - Jain, T.

AU - Rajakannan, T.

AU - Bhat, K.

AU - Gurumadhavrao, P.

AU - Devarakonda, R.

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PY - 2010

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AB - A sensitive and selective high performance liquid chromatographic (HPLC) method was developed and validated for estimation of lamotrigine (CAS 84057-84-1) in human plasma and saliva. The chromatographic separation was achieved with a reversed phase column and a mobile phase consisting of acetonitrile and 20 mM ammoniumacetate buffer pH6.5 (30 : 70) with a flow rate of 1 mL/min. The calibration curve was linear within the working range for both plasma and saliva. The validated method has been successfully applied for a study of lamotrigine in human plasma and saliva to establish the correlation between these two matrices. A scatter plot of plasma versus salivary lamotrigine concentrations showed a good linear relationship between them (Pearson correlation coefficient, r = 0.6832, p <0.001). © ECV · Editio Cantor Verlag.

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