A sensitive and specific HPLC method with fluorescence detector has been developed for the analysis of atenolol in plasma using a reverse phase column (ODS, 250 × 4.6 mm, 10 μm). Atenolol levels were measured using its excitation and emission maxima at 276 nm and 302 nm, respectively. The method has a linearity range of 50-1000 ng/ml and recovery was found to be 95.7%. This method has been successfully used for the bioequivalence study of atenolol tablets (25 mg). Cmax, tmax and AUC0-∞ of the test formulation statistically does not show significant difference from the standard formulation.
|Number of pages||6|
|Journal||Indian Journal of Pharmaceutical Sciences|
|Publication status||Published - 01-05-2000|
All Science Journal Classification (ASJC) codes
- Pharmaceutical Science