A Sensitive High Performance Liquid Chromatography Method for Determination of Bioequivalence of Atenolol Formulations

S. A. Shah, I. S. Rathod, C. J. Shishoo, S. S. Savale, M. C. Satia, K. M. Bhat

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A sensitive and specific HPLC method with fluorescence detector has been developed for the analysis of atenolol in plasma using a reverse phase column (ODS, 250 × 4.6 mm, 10 μm). Atenolol levels were measured using its excitation and emission maxima at 276 nm and 302 nm, respectively. The method has a linearity range of 50-1000 ng/ml and recovery was found to be 95.7%. This method has been successfully used for the bioequivalence study of atenolol tablets (25 mg). Cmax, tmax and AUC0-∞ of the test formulation statistically does not show significant difference from the standard formulation.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
JournalIndian Journal of Pharmaceutical Sciences
Issue number3
Publication statusPublished - 01-05-2000


All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

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