A simple and rapid high performance liquid chromatographic method with fluorescence detection for the estimation of fexofenadine in rat plasma-Application to preclinical pharmacokinetics

Shriram M. Pathak, A. Ranjith Kumar, P. Musmade, N. Udupa

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A sensitive high performance liquid chromatographic (HPLC) method involving fluorescence detection was developed for the determination of fexofenadine (FEX), known to have low oral bioavailability, in rat plasma. In order to understand the effect of various chromatographic factors on the separation of analytes and to simultaneously optimize the resolution and analysis run time, a response surface method was used. The chromatographic separation was achieved using a Supelco C18-DB (250 mm × 4.6 mm I.D./5 μm particle size) column with mobile phase comprising of ammonium acetate buffer and acetonitrile (63:37, v/v), delivered isocratically at a flow rate of 1.0 mL min-1. Diphenhydramine was used as an internal standard (I.S.). The statistical evaluation of the method was examined and the method was found to be precise and accurate with a linearity range of 1-500 ng mL-1 (r > 0.9980). The intra- and inter-day precision studies showed good reproducibility with coefficients of variation (C.V.) less than 12.26%. The advantages of our method are small sample volume (100 μL), short time of analysis (13 min) and a simple sample extraction and clean-up as compared to the previously published methods. The established method provides a reliable bioanalytical methodology to carry out FEX pharmacokinetics in rat plasma.

Original languageEnglish
Pages (from-to)338-346
Number of pages9
JournalTalanta
Volume76
Issue number2
DOIs
Publication statusPublished - 15-07-2008

Fingerprint

fexofenadine
Plasma applications
Pharmacokinetics
Rats
Fluorescence
Plasmas
Diphenhydramine
Liquids
Buffers
Particle size
Flow rate
Particle Size
Biological Availability

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

@article{702e3fa8ba4243c19f36cdf5e98bad6e,
title = "A simple and rapid high performance liquid chromatographic method with fluorescence detection for the estimation of fexofenadine in rat plasma-Application to preclinical pharmacokinetics",
abstract = "A sensitive high performance liquid chromatographic (HPLC) method involving fluorescence detection was developed for the determination of fexofenadine (FEX), known to have low oral bioavailability, in rat plasma. In order to understand the effect of various chromatographic factors on the separation of analytes and to simultaneously optimize the resolution and analysis run time, a response surface method was used. The chromatographic separation was achieved using a Supelco C18-DB (250 mm × 4.6 mm I.D./5 μm particle size) column with mobile phase comprising of ammonium acetate buffer and acetonitrile (63:37, v/v), delivered isocratically at a flow rate of 1.0 mL min-1. Diphenhydramine was used as an internal standard (I.S.). The statistical evaluation of the method was examined and the method was found to be precise and accurate with a linearity range of 1-500 ng mL-1 (r > 0.9980). The intra- and inter-day precision studies showed good reproducibility with coefficients of variation (C.V.) less than 12.26{\%}. The advantages of our method are small sample volume (100 μL), short time of analysis (13 min) and a simple sample extraction and clean-up as compared to the previously published methods. The established method provides a reliable bioanalytical methodology to carry out FEX pharmacokinetics in rat plasma.",
author = "Pathak, {Shriram M.} and Kumar, {A. Ranjith} and P. Musmade and N. Udupa",
year = "2008",
month = "7",
day = "15",
doi = "10.1016/j.talanta.2008.02.047",
language = "English",
volume = "76",
pages = "338--346",
journal = "Talanta",
issn = "0039-9140",
publisher = "Elsevier",
number = "2",

}

A simple and rapid high performance liquid chromatographic method with fluorescence detection for the estimation of fexofenadine in rat plasma-Application to preclinical pharmacokinetics. / Pathak, Shriram M.; Kumar, A. Ranjith; Musmade, P.; Udupa, N.

In: Talanta, Vol. 76, No. 2, 15.07.2008, p. 338-346.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A simple and rapid high performance liquid chromatographic method with fluorescence detection for the estimation of fexofenadine in rat plasma-Application to preclinical pharmacokinetics

AU - Pathak, Shriram M.

AU - Kumar, A. Ranjith

AU - Musmade, P.

AU - Udupa, N.

PY - 2008/7/15

Y1 - 2008/7/15

N2 - A sensitive high performance liquid chromatographic (HPLC) method involving fluorescence detection was developed for the determination of fexofenadine (FEX), known to have low oral bioavailability, in rat plasma. In order to understand the effect of various chromatographic factors on the separation of analytes and to simultaneously optimize the resolution and analysis run time, a response surface method was used. The chromatographic separation was achieved using a Supelco C18-DB (250 mm × 4.6 mm I.D./5 μm particle size) column with mobile phase comprising of ammonium acetate buffer and acetonitrile (63:37, v/v), delivered isocratically at a flow rate of 1.0 mL min-1. Diphenhydramine was used as an internal standard (I.S.). The statistical evaluation of the method was examined and the method was found to be precise and accurate with a linearity range of 1-500 ng mL-1 (r > 0.9980). The intra- and inter-day precision studies showed good reproducibility with coefficients of variation (C.V.) less than 12.26%. The advantages of our method are small sample volume (100 μL), short time of analysis (13 min) and a simple sample extraction and clean-up as compared to the previously published methods. The established method provides a reliable bioanalytical methodology to carry out FEX pharmacokinetics in rat plasma.

AB - A sensitive high performance liquid chromatographic (HPLC) method involving fluorescence detection was developed for the determination of fexofenadine (FEX), known to have low oral bioavailability, in rat plasma. In order to understand the effect of various chromatographic factors on the separation of analytes and to simultaneously optimize the resolution and analysis run time, a response surface method was used. The chromatographic separation was achieved using a Supelco C18-DB (250 mm × 4.6 mm I.D./5 μm particle size) column with mobile phase comprising of ammonium acetate buffer and acetonitrile (63:37, v/v), delivered isocratically at a flow rate of 1.0 mL min-1. Diphenhydramine was used as an internal standard (I.S.). The statistical evaluation of the method was examined and the method was found to be precise and accurate with a linearity range of 1-500 ng mL-1 (r > 0.9980). The intra- and inter-day precision studies showed good reproducibility with coefficients of variation (C.V.) less than 12.26%. The advantages of our method are small sample volume (100 μL), short time of analysis (13 min) and a simple sample extraction and clean-up as compared to the previously published methods. The established method provides a reliable bioanalytical methodology to carry out FEX pharmacokinetics in rat plasma.

UR - http://www.scopus.com/inward/record.url?scp=44149120242&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44149120242&partnerID=8YFLogxK

U2 - 10.1016/j.talanta.2008.02.047

DO - 10.1016/j.talanta.2008.02.047

M3 - Article

VL - 76

SP - 338

EP - 346

JO - Talanta

JF - Talanta

SN - 0039-9140

IS - 2

ER -