A simple kinetic model for growth and biosynthesis of polyhydroxyalkanoate in Bacillus flexus

M. Somashekara Divyashree, Navin K. Rastogi, T. Ramachandriah Shamala

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Polyhydroxyalkanoate (PHA), which is produced by several bacteria, is a biodegradable polymer that has many industrial and medical applications. This study deals with development of a simple kinetic model and modification of the logistic equation that can provide an adequate description of PHA formation process by Bacillus flexus. The parameters studied were kinetics of microbial growth, substrate consumption, and product formation. The microbial growth was described by simplification of Monod's model. A simplified Luedeking-Piret type model could be employed to represent the product kinetics. The kinetic constants were evaluated on the basis of non-linear regression and the differential equations were solved using Runge-Kutta algorithm and MATLAB software. A good agreement was found between the experimental and predicted values, which indicated that the model differential equations could describe the PHA formation and fermentation process. In this study, a modification of the logistic equation has also been attempted for describing the growth of B. flexus.

Original languageEnglish
Pages (from-to)92-98
Number of pages7
JournalNew Biotechnology
Volume26
Issue number1-2
DOIs
Publication statusPublished - 01-11-2009

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Polyhydroxyalkanoates
Biosynthesis
Bacilli
Bacillus
Kinetics
Growth
Logistics
Differential equations
Biodegradable polymers
Medical applications
Fermentation
MATLAB
Industrial applications
Bacteria
Polymers
Software
Logistic Models
Substrates

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Molecular Biology

Cite this

Divyashree, M. Somashekara ; Rastogi, Navin K. ; Shamala, T. Ramachandriah. / A simple kinetic model for growth and biosynthesis of polyhydroxyalkanoate in Bacillus flexus. In: New Biotechnology. 2009 ; Vol. 26, No. 1-2. pp. 92-98.
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A simple kinetic model for growth and biosynthesis of polyhydroxyalkanoate in Bacillus flexus. / Divyashree, M. Somashekara; Rastogi, Navin K.; Shamala, T. Ramachandriah.

In: New Biotechnology, Vol. 26, No. 1-2, 01.11.2009, p. 92-98.

Research output: Contribution to journalArticle

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AB - Polyhydroxyalkanoate (PHA), which is produced by several bacteria, is a biodegradable polymer that has many industrial and medical applications. This study deals with development of a simple kinetic model and modification of the logistic equation that can provide an adequate description of PHA formation process by Bacillus flexus. The parameters studied were kinetics of microbial growth, substrate consumption, and product formation. The microbial growth was described by simplification of Monod's model. A simplified Luedeking-Piret type model could be employed to represent the product kinetics. The kinetic constants were evaluated on the basis of non-linear regression and the differential equations were solved using Runge-Kutta algorithm and MATLAB software. A good agreement was found between the experimental and predicted values, which indicated that the model differential equations could describe the PHA formation and fermentation process. In this study, a modification of the logistic equation has also been attempted for describing the growth of B. flexus.

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