A study of the PfNT3 in Plasmodium falciparum

Ahu Pratima Kumari, Panda Maheswar, Patra Satyajit, Das Sidhartha, Satyamoorthy Kapaettu, Mohanty Dipika

    Research output: Contribution to journalArticle

    Abstract

    Previous genetic studies demonstrated that survival and proliferation of Plasmodium falciparum parasites is dependent on salvage of essential purines from the host. Plasmodium falciparum, the causative agent of the most lethal form of human malaria lacks the enzymes required for de novo synthesis of purines. Analysis of the hypothetical nucleoside/nucleobase transporter protein, the gene product of PfNT3 (PF14_0662) gene in P. falciparum parasites was carried out by localisation, in view of a novel chemotherapeutic target. Immunoblotting, immunofluorescent and immunoelectron microscopic localization of PfNT3 was demonstrated using polyclonal antiserum in in vitro cultured Plasmodium falciparum parasites, propagated in human red blood cells. PfNT3 protein, the translated product of PfNT3 gene was detected in intraerythrocytic ring, trophozoite, and schizont stages. PfNT3 was localized primarily to the PPM (Parasite Plasma Membrane). The endogenous PfNT3 putative nucleoside transporter with the predominant location to the parasite plasma membrane may serve not only as routes for targeting of purine analogs/cytotoxic agents into the intracellular parasite but may also serve as drug targets. Being genome encoded the vital transporter protein can be prevented from expression by silencing of the gene, validating it to be a novel drug target.
    Original languageEnglish
    JournalMedicine & Health
    Publication statusPublished - 2015

    Fingerprint

    Plasmodium falciparum
    Parasites
    Nucleoside Transport Proteins
    Purines
    Cell Membrane
    Schizonts
    Trophozoites
    Proteins
    Cytotoxins
    Gene Silencing
    Immunoblotting
    Pharmaceutical Preparations
    Genes
    Malaria
    Immune Sera
    Erythrocytes
    Genome
    Gene Expression
    Survival
    Enzymes

    Cite this

    Kumari, A. P., Maheswar, P., Satyajit, P., Sidhartha, D., Kapaettu, S., & Dipika, M. (2015). A study of the PfNT3 in Plasmodium falciparum. Medicine & Health.
    Kumari, Ahu Pratima ; Maheswar, Panda ; Satyajit, Patra ; Sidhartha, Das ; Kapaettu, Satyamoorthy ; Dipika, Mohanty. / A study of the PfNT3 in Plasmodium falciparum. In: Medicine & Health. 2015.
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    abstract = "Previous genetic studies demonstrated that survival and proliferation of Plasmodium falciparum parasites is dependent on salvage of essential purines from the host. Plasmodium falciparum, the causative agent of the most lethal form of human malaria lacks the enzymes required for de novo synthesis of purines. Analysis of the hypothetical nucleoside/nucleobase transporter protein, the gene product of PfNT3 (PF14_0662) gene in P. falciparum parasites was carried out by localisation, in view of a novel chemotherapeutic target. Immunoblotting, immunofluorescent and immunoelectron microscopic localization of PfNT3 was demonstrated using polyclonal antiserum in in vitro cultured Plasmodium falciparum parasites, propagated in human red blood cells. PfNT3 protein, the translated product of PfNT3 gene was detected in intraerythrocytic ring, trophozoite, and schizont stages. PfNT3 was localized primarily to the PPM (Parasite Plasma Membrane). The endogenous PfNT3 putative nucleoside transporter with the predominant location to the parasite plasma membrane may serve not only as routes for targeting of purine analogs/cytotoxic agents into the intracellular parasite but may also serve as drug targets. Being genome encoded the vital transporter protein can be prevented from expression by silencing of the gene, validating it to be a novel drug target.",
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    Kumari, AP, Maheswar, P, Satyajit, P, Sidhartha, D, Kapaettu, S & Dipika, M 2015, 'A study of the PfNT3 in Plasmodium falciparum', Medicine & Health.

    A study of the PfNT3 in Plasmodium falciparum. / Kumari, Ahu Pratima; Maheswar, Panda; Satyajit, Patra; Sidhartha, Das; Kapaettu, Satyamoorthy; Dipika, Mohanty.

    In: Medicine & Health, 2015.

    Research output: Contribution to journalArticle

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    T1 - A study of the PfNT3 in Plasmodium falciparum

    AU - Kumari, Ahu Pratima

    AU - Maheswar, Panda

    AU - Satyajit, Patra

    AU - Sidhartha, Das

    AU - Kapaettu, Satyamoorthy

    AU - Dipika, Mohanty

    PY - 2015

    Y1 - 2015

    N2 - Previous genetic studies demonstrated that survival and proliferation of Plasmodium falciparum parasites is dependent on salvage of essential purines from the host. Plasmodium falciparum, the causative agent of the most lethal form of human malaria lacks the enzymes required for de novo synthesis of purines. Analysis of the hypothetical nucleoside/nucleobase transporter protein, the gene product of PfNT3 (PF14_0662) gene in P. falciparum parasites was carried out by localisation, in view of a novel chemotherapeutic target. Immunoblotting, immunofluorescent and immunoelectron microscopic localization of PfNT3 was demonstrated using polyclonal antiserum in in vitro cultured Plasmodium falciparum parasites, propagated in human red blood cells. PfNT3 protein, the translated product of PfNT3 gene was detected in intraerythrocytic ring, trophozoite, and schizont stages. PfNT3 was localized primarily to the PPM (Parasite Plasma Membrane). The endogenous PfNT3 putative nucleoside transporter with the predominant location to the parasite plasma membrane may serve not only as routes for targeting of purine analogs/cytotoxic agents into the intracellular parasite but may also serve as drug targets. Being genome encoded the vital transporter protein can be prevented from expression by silencing of the gene, validating it to be a novel drug target.

    AB - Previous genetic studies demonstrated that survival and proliferation of Plasmodium falciparum parasites is dependent on salvage of essential purines from the host. Plasmodium falciparum, the causative agent of the most lethal form of human malaria lacks the enzymes required for de novo synthesis of purines. Analysis of the hypothetical nucleoside/nucleobase transporter protein, the gene product of PfNT3 (PF14_0662) gene in P. falciparum parasites was carried out by localisation, in view of a novel chemotherapeutic target. Immunoblotting, immunofluorescent and immunoelectron microscopic localization of PfNT3 was demonstrated using polyclonal antiserum in in vitro cultured Plasmodium falciparum parasites, propagated in human red blood cells. PfNT3 protein, the translated product of PfNT3 gene was detected in intraerythrocytic ring, trophozoite, and schizont stages. PfNT3 was localized primarily to the PPM (Parasite Plasma Membrane). The endogenous PfNT3 putative nucleoside transporter with the predominant location to the parasite plasma membrane may serve not only as routes for targeting of purine analogs/cytotoxic agents into the intracellular parasite but may also serve as drug targets. Being genome encoded the vital transporter protein can be prevented from expression by silencing of the gene, validating it to be a novel drug target.

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    Kumari AP, Maheswar P, Satyajit P, Sidhartha D, Kapaettu S, Dipika M. A study of the PfNT3 in Plasmodium falciparum. Medicine & Health. 2015.