A study on biofilm production and antifungal drug resistance among Candida species from vulvovaginal and bloodstream infections

Sanyuktha Tulasidas, Pooja Rao, Sevitha Bhat, Radhakrishna Manipura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Introduction: Candida species, one among the opportunistic fungi, has become a common pathogen causing vaginal thrush and nosocomial bloodstream infections (BSIs). This study aims to evaluate the prevalence and antifungal susceptibility of various Candida species and slime production by Candida species in BSIs and vulvovaginal candidiasis (VVC). Materials and methods: A total of 176 samples were collected for a period of 1 year. Antifungal susceptibility testing and biofilm production testing were performed by the Kirby-Bauer method and crystal violet assay, respectively. Results: Out of 176 samples, 74 (42%) were from BSIs and 102 (58%) were from VVC. The biofilm production was comparatively high in blood isolates, 55 (74%), than cervical isolates, 45 (44%). Increase in the trends of non-albicans Candida (NAC) species was seen in our setup. Good susceptibility rates were seen among Candida species, 82.38% to voriconazole and an increasing resistance pattern of 26.13% to fluconazole. Conclusion: Speciation of Candida becomes important as the prevalence of NAC is increasing. Antifungal susceptibility testing by the disk diffusion method is cost effective and should be adopted in routine testing as there is an increasing azole resistance, especially in invasive NAC infections. In this study, there was no correlation of antifungal drugs with the biofilm production.

Original languageEnglish
Pages (from-to)2443-2448
Number of pages6
JournalInfection and Drug Resistance
Volume11
DOIs
Publication statusPublished - 01-01-2018

Fingerprint

Fungal Drug Resistance
Biofilms
Candida
Infection
Vulvovaginal Candidiasis
Gentian Violet
Oral Candidiasis
Azoles
Fluconazole
Cross Infection
Fungi

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

@article{bb0258b914814aba9648ec4bc1ba86cd,
title = "A study on biofilm production and antifungal drug resistance among Candida species from vulvovaginal and bloodstream infections",
abstract = "Introduction: Candida species, one among the opportunistic fungi, has become a common pathogen causing vaginal thrush and nosocomial bloodstream infections (BSIs). This study aims to evaluate the prevalence and antifungal susceptibility of various Candida species and slime production by Candida species in BSIs and vulvovaginal candidiasis (VVC). Materials and methods: A total of 176 samples were collected for a period of 1 year. Antifungal susceptibility testing and biofilm production testing were performed by the Kirby-Bauer method and crystal violet assay, respectively. Results: Out of 176 samples, 74 (42{\%}) were from BSIs and 102 (58{\%}) were from VVC. The biofilm production was comparatively high in blood isolates, 55 (74{\%}), than cervical isolates, 45 (44{\%}). Increase in the trends of non-albicans Candida (NAC) species was seen in our setup. Good susceptibility rates were seen among Candida species, 82.38{\%} to voriconazole and an increasing resistance pattern of 26.13{\%} to fluconazole. Conclusion: Speciation of Candida becomes important as the prevalence of NAC is increasing. Antifungal susceptibility testing by the disk diffusion method is cost effective and should be adopted in routine testing as there is an increasing azole resistance, especially in invasive NAC infections. In this study, there was no correlation of antifungal drugs with the biofilm production.",
author = "Sanyuktha Tulasidas and Pooja Rao and Sevitha Bhat and Radhakrishna Manipura",
year = "2018",
month = "1",
day = "1",
doi = "10.2147/IDR.S179462",
language = "English",
volume = "11",
pages = "2443--2448",
journal = "Infection and Drug Resistance",
issn = "1178-6973",
publisher = "Dove Medical Press Ltd.",

}

A study on biofilm production and antifungal drug resistance among Candida species from vulvovaginal and bloodstream infections. / Tulasidas, Sanyuktha; Rao, Pooja; Bhat, Sevitha; Manipura, Radhakrishna.

In: Infection and Drug Resistance, Vol. 11, 01.01.2018, p. 2443-2448.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A study on biofilm production and antifungal drug resistance among Candida species from vulvovaginal and bloodstream infections

AU - Tulasidas, Sanyuktha

AU - Rao, Pooja

AU - Bhat, Sevitha

AU - Manipura, Radhakrishna

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Introduction: Candida species, one among the opportunistic fungi, has become a common pathogen causing vaginal thrush and nosocomial bloodstream infections (BSIs). This study aims to evaluate the prevalence and antifungal susceptibility of various Candida species and slime production by Candida species in BSIs and vulvovaginal candidiasis (VVC). Materials and methods: A total of 176 samples were collected for a period of 1 year. Antifungal susceptibility testing and biofilm production testing were performed by the Kirby-Bauer method and crystal violet assay, respectively. Results: Out of 176 samples, 74 (42%) were from BSIs and 102 (58%) were from VVC. The biofilm production was comparatively high in blood isolates, 55 (74%), than cervical isolates, 45 (44%). Increase in the trends of non-albicans Candida (NAC) species was seen in our setup. Good susceptibility rates were seen among Candida species, 82.38% to voriconazole and an increasing resistance pattern of 26.13% to fluconazole. Conclusion: Speciation of Candida becomes important as the prevalence of NAC is increasing. Antifungal susceptibility testing by the disk diffusion method is cost effective and should be adopted in routine testing as there is an increasing azole resistance, especially in invasive NAC infections. In this study, there was no correlation of antifungal drugs with the biofilm production.

AB - Introduction: Candida species, one among the opportunistic fungi, has become a common pathogen causing vaginal thrush and nosocomial bloodstream infections (BSIs). This study aims to evaluate the prevalence and antifungal susceptibility of various Candida species and slime production by Candida species in BSIs and vulvovaginal candidiasis (VVC). Materials and methods: A total of 176 samples were collected for a period of 1 year. Antifungal susceptibility testing and biofilm production testing were performed by the Kirby-Bauer method and crystal violet assay, respectively. Results: Out of 176 samples, 74 (42%) were from BSIs and 102 (58%) were from VVC. The biofilm production was comparatively high in blood isolates, 55 (74%), than cervical isolates, 45 (44%). Increase in the trends of non-albicans Candida (NAC) species was seen in our setup. Good susceptibility rates were seen among Candida species, 82.38% to voriconazole and an increasing resistance pattern of 26.13% to fluconazole. Conclusion: Speciation of Candida becomes important as the prevalence of NAC is increasing. Antifungal susceptibility testing by the disk diffusion method is cost effective and should be adopted in routine testing as there is an increasing azole resistance, especially in invasive NAC infections. In this study, there was no correlation of antifungal drugs with the biofilm production.

UR - http://www.scopus.com/inward/record.url?scp=85057612768&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85057612768&partnerID=8YFLogxK

U2 - 10.2147/IDR.S179462

DO - 10.2147/IDR.S179462

M3 - Article

AN - SCOPUS:85057612768

VL - 11

SP - 2443

EP - 2448

JO - Infection and Drug Resistance

JF - Infection and Drug Resistance

SN - 1178-6973

ER -