A study on the protective effect of single dose of N-2- mercaptopropionyl glycine administration prior to renal reperfusion on wistar rats

N. A. Vinodini, Y. Tripathi, C. V. Raghuveer, Asha Kamath, C. Ramaswam

Research output: Contribution to journalArticle

Abstract

N-2-mercaptopropionylglycine (NMG) is a powerful antioxidant which inhibits synthesize of superoxide radicals. It has been tested as a preventive agent against metabolic and structural damage induced during ischemia/reperfusion (I/R) process. To validate the efficacy of NMG on renal tissue this work was devised. During the experiment, the wistar strain rats were divided into five groups; the group I as normal control, the group II & III as I/R controls, and the group IV & V as I/R treated groups. The I/R control and I/R treated groups were subjected to ischemia and reperfusion either for 10 minutes (Gr.II & IV) or 90 min. (Gr.III & V). The I/R treated groups in addition received N-2- mercaptopropionylglycine (50mg/kg bw) intravenously prior to the onset of reperfusion. The results suggest that 60 minutes of ischemia followed by 10 (Gr.II) and 90 minutes (Gr.III) of reperfusion increased lipid peroxidation (MDA), and decreased glutathione (GSH), & Superoxide dismutase levels in the renal tissues compared to normal control. However, the GSH and SOD levels were higher and the lipid peroxidation level was lower in I/R treated groups than I/R controls. Thus, the present study confirms the protective effect of N-2- mercaptopropionylglycine on the reperfusion injury and also extends that protective effect on renal tissue.

Original languageEnglish
Pages (from-to)149-153
Number of pages5
JournalAustralian Journal of Basic and Applied Sciences
Volume5
Issue number4
Publication statusPublished - 04-2011

Fingerprint

Glycine
Reperfusion
Wistar Rats
Ischemia
Kidney
Tiopronin
Lipid Peroxidation
Control Groups
Reperfusion Injury
Superoxides
Superoxide Dismutase
Glutathione
Antioxidants

All Science Journal Classification (ASJC) codes

  • General

Cite this

@article{2fcb885ffeab4f65a2ba7c1676dbedf8,
title = "A study on the protective effect of single dose of N-2- mercaptopropionyl glycine administration prior to renal reperfusion on wistar rats",
abstract = "N-2-mercaptopropionylglycine (NMG) is a powerful antioxidant which inhibits synthesize of superoxide radicals. It has been tested as a preventive agent against metabolic and structural damage induced during ischemia/reperfusion (I/R) process. To validate the efficacy of NMG on renal tissue this work was devised. During the experiment, the wistar strain rats were divided into five groups; the group I as normal control, the group II & III as I/R controls, and the group IV & V as I/R treated groups. The I/R control and I/R treated groups were subjected to ischemia and reperfusion either for 10 minutes (Gr.II & IV) or 90 min. (Gr.III & V). The I/R treated groups in addition received N-2- mercaptopropionylglycine (50mg/kg bw) intravenously prior to the onset of reperfusion. The results suggest that 60 minutes of ischemia followed by 10 (Gr.II) and 90 minutes (Gr.III) of reperfusion increased lipid peroxidation (MDA), and decreased glutathione (GSH), & Superoxide dismutase levels in the renal tissues compared to normal control. However, the GSH and SOD levels were higher and the lipid peroxidation level was lower in I/R treated groups than I/R controls. Thus, the present study confirms the protective effect of N-2- mercaptopropionylglycine on the reperfusion injury and also extends that protective effect on renal tissue.",
author = "Vinodini, {N. A.} and Y. Tripathi and Raghuveer, {C. V.} and Asha Kamath and C. Ramaswam",
year = "2011",
month = "4",
language = "English",
volume = "5",
pages = "149--153",
journal = "Australian Journal of Basic and Applied Sciences",
issn = "1991-8178",
publisher = "INSInet Publications",
number = "4",

}

TY - JOUR

T1 - A study on the protective effect of single dose of N-2- mercaptopropionyl glycine administration prior to renal reperfusion on wistar rats

AU - Vinodini, N. A.

AU - Tripathi, Y.

AU - Raghuveer, C. V.

AU - Kamath, Asha

AU - Ramaswam, C.

PY - 2011/4

Y1 - 2011/4

N2 - N-2-mercaptopropionylglycine (NMG) is a powerful antioxidant which inhibits synthesize of superoxide radicals. It has been tested as a preventive agent against metabolic and structural damage induced during ischemia/reperfusion (I/R) process. To validate the efficacy of NMG on renal tissue this work was devised. During the experiment, the wistar strain rats were divided into five groups; the group I as normal control, the group II & III as I/R controls, and the group IV & V as I/R treated groups. The I/R control and I/R treated groups were subjected to ischemia and reperfusion either for 10 minutes (Gr.II & IV) or 90 min. (Gr.III & V). The I/R treated groups in addition received N-2- mercaptopropionylglycine (50mg/kg bw) intravenously prior to the onset of reperfusion. The results suggest that 60 minutes of ischemia followed by 10 (Gr.II) and 90 minutes (Gr.III) of reperfusion increased lipid peroxidation (MDA), and decreased glutathione (GSH), & Superoxide dismutase levels in the renal tissues compared to normal control. However, the GSH and SOD levels were higher and the lipid peroxidation level was lower in I/R treated groups than I/R controls. Thus, the present study confirms the protective effect of N-2- mercaptopropionylglycine on the reperfusion injury and also extends that protective effect on renal tissue.

AB - N-2-mercaptopropionylglycine (NMG) is a powerful antioxidant which inhibits synthesize of superoxide radicals. It has been tested as a preventive agent against metabolic and structural damage induced during ischemia/reperfusion (I/R) process. To validate the efficacy of NMG on renal tissue this work was devised. During the experiment, the wistar strain rats were divided into five groups; the group I as normal control, the group II & III as I/R controls, and the group IV & V as I/R treated groups. The I/R control and I/R treated groups were subjected to ischemia and reperfusion either for 10 minutes (Gr.II & IV) or 90 min. (Gr.III & V). The I/R treated groups in addition received N-2- mercaptopropionylglycine (50mg/kg bw) intravenously prior to the onset of reperfusion. The results suggest that 60 minutes of ischemia followed by 10 (Gr.II) and 90 minutes (Gr.III) of reperfusion increased lipid peroxidation (MDA), and decreased glutathione (GSH), & Superoxide dismutase levels in the renal tissues compared to normal control. However, the GSH and SOD levels were higher and the lipid peroxidation level was lower in I/R treated groups than I/R controls. Thus, the present study confirms the protective effect of N-2- mercaptopropionylglycine on the reperfusion injury and also extends that protective effect on renal tissue.

UR - http://www.scopus.com/inward/record.url?scp=79954515308&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79954515308&partnerID=8YFLogxK

M3 - Article

VL - 5

SP - 149

EP - 153

JO - Australian Journal of Basic and Applied Sciences

JF - Australian Journal of Basic and Applied Sciences

SN - 1991-8178

IS - 4

ER -