A versatile method for the removal of melanin from ribonucleic acids in melanocytic cells

K. Satyamoorthy, G. Li, P. A. Van Belle, D. E. Elder, M. Herlyn

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Melanin pigments often co-purify during preparation of nucleic acids from cells or tissues of melanocytic origin. Contaminating melanin can severely impede subsequent analyses of RNA. We attempted to eliminate melanin in RNA preparations using selected gel matrices. We show here that co-purified melanin pigments can be largely eliminated from RNA samples after passing through polyacrylamide-based beads (Bio-Gel P-60). After isolation from the pigment-containing cells or tissues, RNA was subsequently processed through batch or column purification under acidic pH conditions. The resulting RNA was devoid of contaminating melanin pigments and amenable to molecular reactions such as polymerase chain reaction and cDNA synthesis by reverse transcriptase. Although the process results in some loss of input RNA, this purification procedure is simple, robust and can easily be adopted in any laboratory for the molecular analysis of RNA that requires removal of melanin contamination.

    Original languageEnglish
    Pages (from-to)449-452
    Number of pages4
    JournalMelanoma Research
    Volume12
    Issue number5
    DOIs
    Publication statusPublished - 09-2002

    Fingerprint

    Melanins
    RNA
    Gels
    RNA-Directed DNA Polymerase
    Nucleic Acids
    Complementary DNA
    Polymerase Chain Reaction

    All Science Journal Classification (ASJC) codes

    • Cancer Research
    • Dermatology

    Cite this

    Satyamoorthy, K. ; Li, G. ; Van Belle, P. A. ; Elder, D. E. ; Herlyn, M. / A versatile method for the removal of melanin from ribonucleic acids in melanocytic cells. In: Melanoma Research. 2002 ; Vol. 12, No. 5. pp. 449-452.
    @article{e51211dbd81648cd8ec783220b7e484c,
    title = "A versatile method for the removal of melanin from ribonucleic acids in melanocytic cells",
    abstract = "Melanin pigments often co-purify during preparation of nucleic acids from cells or tissues of melanocytic origin. Contaminating melanin can severely impede subsequent analyses of RNA. We attempted to eliminate melanin in RNA preparations using selected gel matrices. We show here that co-purified melanin pigments can be largely eliminated from RNA samples after passing through polyacrylamide-based beads (Bio-Gel P-60). After isolation from the pigment-containing cells or tissues, RNA was subsequently processed through batch or column purification under acidic pH conditions. The resulting RNA was devoid of contaminating melanin pigments and amenable to molecular reactions such as polymerase chain reaction and cDNA synthesis by reverse transcriptase. Although the process results in some loss of input RNA, this purification procedure is simple, robust and can easily be adopted in any laboratory for the molecular analysis of RNA that requires removal of melanin contamination.",
    author = "K. Satyamoorthy and G. Li and {Van Belle}, {P. A.} and Elder, {D. E.} and M. Herlyn",
    year = "2002",
    month = "9",
    doi = "10.1097/00008390-200209000-00006",
    language = "English",
    volume = "12",
    pages = "449--452",
    journal = "Melanoma Research",
    issn = "0960-8931",
    publisher = "Lippincott Williams and Wilkins",
    number = "5",

    }

    A versatile method for the removal of melanin from ribonucleic acids in melanocytic cells. / Satyamoorthy, K.; Li, G.; Van Belle, P. A.; Elder, D. E.; Herlyn, M.

    In: Melanoma Research, Vol. 12, No. 5, 09.2002, p. 449-452.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - A versatile method for the removal of melanin from ribonucleic acids in melanocytic cells

    AU - Satyamoorthy, K.

    AU - Li, G.

    AU - Van Belle, P. A.

    AU - Elder, D. E.

    AU - Herlyn, M.

    PY - 2002/9

    Y1 - 2002/9

    N2 - Melanin pigments often co-purify during preparation of nucleic acids from cells or tissues of melanocytic origin. Contaminating melanin can severely impede subsequent analyses of RNA. We attempted to eliminate melanin in RNA preparations using selected gel matrices. We show here that co-purified melanin pigments can be largely eliminated from RNA samples after passing through polyacrylamide-based beads (Bio-Gel P-60). After isolation from the pigment-containing cells or tissues, RNA was subsequently processed through batch or column purification under acidic pH conditions. The resulting RNA was devoid of contaminating melanin pigments and amenable to molecular reactions such as polymerase chain reaction and cDNA synthesis by reverse transcriptase. Although the process results in some loss of input RNA, this purification procedure is simple, robust and can easily be adopted in any laboratory for the molecular analysis of RNA that requires removal of melanin contamination.

    AB - Melanin pigments often co-purify during preparation of nucleic acids from cells or tissues of melanocytic origin. Contaminating melanin can severely impede subsequent analyses of RNA. We attempted to eliminate melanin in RNA preparations using selected gel matrices. We show here that co-purified melanin pigments can be largely eliminated from RNA samples after passing through polyacrylamide-based beads (Bio-Gel P-60). After isolation from the pigment-containing cells or tissues, RNA was subsequently processed through batch or column purification under acidic pH conditions. The resulting RNA was devoid of contaminating melanin pigments and amenable to molecular reactions such as polymerase chain reaction and cDNA synthesis by reverse transcriptase. Although the process results in some loss of input RNA, this purification procedure is simple, robust and can easily be adopted in any laboratory for the molecular analysis of RNA that requires removal of melanin contamination.

    UR - http://www.scopus.com/inward/record.url?scp=1842858244&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=1842858244&partnerID=8YFLogxK

    U2 - 10.1097/00008390-200209000-00006

    DO - 10.1097/00008390-200209000-00006

    M3 - Article

    AN - SCOPUS:1842858244

    VL - 12

    SP - 449

    EP - 452

    JO - Melanoma Research

    JF - Melanoma Research

    SN - 0960-8931

    IS - 5

    ER -