Ability of deoxyribonucleic acid-damaged sperm to withstand freeze-thaw-induced damage during cryopreservation

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2 Citations (Scopus)

Abstract

Objective: To understand the association between sperm DNA damage and the ability of DNA-damaged sperm to withstand the freeze-thaw process during cryopreservation. Design: Experimental prospective study. Setting(s): Embryology research laboratory. Animals: Eight- to 12-week-old Swiss strain male albino mice (Mus musculus). Intervention(s): Sperm carrying a known amount of DNA damage was subjected to cryopreservation and thereafter evaluated for survival and freeze-thaw-induced DNA damage. Main Outcome Measure(s): Elucidation of association between the amount of initial sperm DNA damage, cryosurvival, and freeze-thaw-induced DNA modification. Result(s): A strong correlation (R = -0.87) was observed between the amount of initial sperm DNA damage and postthaw survival. However, no significant enhancement in DNA damage was observed by the cryopreservation of spermatozoa with various amounts of DNA damage. Conclusion(s): Cryopreservation of DNA-damaged sperm does not deteriorate the DNA quality, but sperm survival is compromised.

Original languageEnglish
Pages (from-to)959-963
Number of pages5
JournalFertility and Sterility
Volume92
Issue number3
DOIs
Publication statusPublished - 09-2009

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Cryopreservation
Spermatozoa
DNA Damage
DNA
Embryology
Research Design
Outcome Assessment (Health Care)
Prospective Studies
Research

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

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title = "Ability of deoxyribonucleic acid-damaged sperm to withstand freeze-thaw-induced damage during cryopreservation",
abstract = "Objective: To understand the association between sperm DNA damage and the ability of DNA-damaged sperm to withstand the freeze-thaw process during cryopreservation. Design: Experimental prospective study. Setting(s): Embryology research laboratory. Animals: Eight- to 12-week-old Swiss strain male albino mice (Mus musculus). Intervention(s): Sperm carrying a known amount of DNA damage was subjected to cryopreservation and thereafter evaluated for survival and freeze-thaw-induced DNA damage. Main Outcome Measure(s): Elucidation of association between the amount of initial sperm DNA damage, cryosurvival, and freeze-thaw-induced DNA modification. Result(s): A strong correlation (R = -0.87) was observed between the amount of initial sperm DNA damage and postthaw survival. However, no significant enhancement in DNA damage was observed by the cryopreservation of spermatozoa with various amounts of DNA damage. Conclusion(s): Cryopreservation of DNA-damaged sperm does not deteriorate the DNA quality, but sperm survival is compromised.",
author = "Adiga, {Satish Kumar} and Zaheer Khan and Dinesh Upadhya and Guruprasad Kalthur and Pratap Kumar",
year = "2009",
month = "9",
doi = "10.1016/j.fertnstert.2008.07.1754",
language = "English",
volume = "92",
pages = "959--963",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "3",

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T1 - Ability of deoxyribonucleic acid-damaged sperm to withstand freeze-thaw-induced damage during cryopreservation

AU - Adiga, Satish Kumar

AU - Khan, Zaheer

AU - Upadhya, Dinesh

AU - Kalthur, Guruprasad

AU - Kumar, Pratap

PY - 2009/9

Y1 - 2009/9

N2 - Objective: To understand the association between sperm DNA damage and the ability of DNA-damaged sperm to withstand the freeze-thaw process during cryopreservation. Design: Experimental prospective study. Setting(s): Embryology research laboratory. Animals: Eight- to 12-week-old Swiss strain male albino mice (Mus musculus). Intervention(s): Sperm carrying a known amount of DNA damage was subjected to cryopreservation and thereafter evaluated for survival and freeze-thaw-induced DNA damage. Main Outcome Measure(s): Elucidation of association between the amount of initial sperm DNA damage, cryosurvival, and freeze-thaw-induced DNA modification. Result(s): A strong correlation (R = -0.87) was observed between the amount of initial sperm DNA damage and postthaw survival. However, no significant enhancement in DNA damage was observed by the cryopreservation of spermatozoa with various amounts of DNA damage. Conclusion(s): Cryopreservation of DNA-damaged sperm does not deteriorate the DNA quality, but sperm survival is compromised.

AB - Objective: To understand the association between sperm DNA damage and the ability of DNA-damaged sperm to withstand the freeze-thaw process during cryopreservation. Design: Experimental prospective study. Setting(s): Embryology research laboratory. Animals: Eight- to 12-week-old Swiss strain male albino mice (Mus musculus). Intervention(s): Sperm carrying a known amount of DNA damage was subjected to cryopreservation and thereafter evaluated for survival and freeze-thaw-induced DNA damage. Main Outcome Measure(s): Elucidation of association between the amount of initial sperm DNA damage, cryosurvival, and freeze-thaw-induced DNA modification. Result(s): A strong correlation (R = -0.87) was observed between the amount of initial sperm DNA damage and postthaw survival. However, no significant enhancement in DNA damage was observed by the cryopreservation of spermatozoa with various amounts of DNA damage. Conclusion(s): Cryopreservation of DNA-damaged sperm does not deteriorate the DNA quality, but sperm survival is compromised.

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