Absorption spectroscopy for the quantification of serum thyroglobulin and thyroglobulin antibody levels in differentiated thyroid carcinoma patients

A pilot study

Sudha D. Kamath, Sujatha Bhat, K. Rajesh, K. Srujan, R. Vrunda, Nandini Pandit

Research output: Contribution to journalArticle

Abstract

Thyroglobulin (Tg) is a glycoprotein specifically synthesized by follicular thyroid epithelium. After thyroidectomy, serum Tg is a specific and sensitive marker for the presence of functioning thyroid tissue, and its measurement is fundamental in the follow-up of patients affected by differentiated thyroid carcinomas (DTCs). Serum antibodies against thyroglobulin (TgAbs) are common in patients with differentiated thyroid cancer (DTC) and can interfere in thyroglobulin (Tg) assays. Unfortunately, serum Tg measurement becomes useless in approximately 10-20% of DTC cases who are positive for anti-Tg antibodies that interfere with the Tg measurement. To overcome this problem, in the present study, we have used absorption spectroscopy technique for the quantification of Tg and TgAb. As a pilot study, current work was carried out on a limited number of 15 serum samples (5 normal and 10 DTC patients) and their absorption spectra were recorded in the UV-VIS region (200 nm-800 nm). Two peaks correspond to immunoglobulin antibody (IgG) were identified in the absorption spectrum of patient's serum sample and the areas/band sums (FWHM) of these two peaks were used to quantify the level of TgAb. The measured values of TgAb by enzyme-linked immunosorbent assay (ELISA) were matching well with the band sums of the IgG peaks proving the good sensitivity of the absorption technique.

Original languageEnglish
Pages (from-to)262-266
Number of pages5
JournalJournal of Medical Imaging and Health Informatics
Volume4
Issue number2
DOIs
Publication statusPublished - 01-01-2014

Fingerprint

Thyroglobulin
Thyroid Neoplasms
Spectrum Analysis
Antibodies
Serum
Thyroid Gland
Immunoglobulin G
Thyroidectomy
Immunoglobulins
Glycoproteins
Epithelium
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging
  • Health Informatics

Cite this

@article{6e04bcf25812450e966b368ef9780bad,
title = "Absorption spectroscopy for the quantification of serum thyroglobulin and thyroglobulin antibody levels in differentiated thyroid carcinoma patients: A pilot study",
abstract = "Thyroglobulin (Tg) is a glycoprotein specifically synthesized by follicular thyroid epithelium. After thyroidectomy, serum Tg is a specific and sensitive marker for the presence of functioning thyroid tissue, and its measurement is fundamental in the follow-up of patients affected by differentiated thyroid carcinomas (DTCs). Serum antibodies against thyroglobulin (TgAbs) are common in patients with differentiated thyroid cancer (DTC) and can interfere in thyroglobulin (Tg) assays. Unfortunately, serum Tg measurement becomes useless in approximately 10-20{\%} of DTC cases who are positive for anti-Tg antibodies that interfere with the Tg measurement. To overcome this problem, in the present study, we have used absorption spectroscopy technique for the quantification of Tg and TgAb. As a pilot study, current work was carried out on a limited number of 15 serum samples (5 normal and 10 DTC patients) and their absorption spectra were recorded in the UV-VIS region (200 nm-800 nm). Two peaks correspond to immunoglobulin antibody (IgG) were identified in the absorption spectrum of patient's serum sample and the areas/band sums (FWHM) of these two peaks were used to quantify the level of TgAb. The measured values of TgAb by enzyme-linked immunosorbent assay (ELISA) were matching well with the band sums of the IgG peaks proving the good sensitivity of the absorption technique.",
author = "Kamath, {Sudha D.} and Sujatha Bhat and K. Rajesh and K. Srujan and R. Vrunda and Nandini Pandit",
year = "2014",
month = "1",
day = "1",
doi = "10.1166/jmihi.2014.1252",
language = "English",
volume = "4",
pages = "262--266",
journal = "Journal of Medical Imaging and Health Informatics",
issn = "2156-7018",
publisher = "American Scientific Publishers",
number = "2",

}

Absorption spectroscopy for the quantification of serum thyroglobulin and thyroglobulin antibody levels in differentiated thyroid carcinoma patients : A pilot study. / Kamath, Sudha D.; Bhat, Sujatha; Rajesh, K.; Srujan, K.; Vrunda, R.; Pandit, Nandini.

In: Journal of Medical Imaging and Health Informatics, Vol. 4, No. 2, 01.01.2014, p. 262-266.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Absorption spectroscopy for the quantification of serum thyroglobulin and thyroglobulin antibody levels in differentiated thyroid carcinoma patients

T2 - A pilot study

AU - Kamath, Sudha D.

AU - Bhat, Sujatha

AU - Rajesh, K.

AU - Srujan, K.

AU - Vrunda, R.

AU - Pandit, Nandini

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Thyroglobulin (Tg) is a glycoprotein specifically synthesized by follicular thyroid epithelium. After thyroidectomy, serum Tg is a specific and sensitive marker for the presence of functioning thyroid tissue, and its measurement is fundamental in the follow-up of patients affected by differentiated thyroid carcinomas (DTCs). Serum antibodies against thyroglobulin (TgAbs) are common in patients with differentiated thyroid cancer (DTC) and can interfere in thyroglobulin (Tg) assays. Unfortunately, serum Tg measurement becomes useless in approximately 10-20% of DTC cases who are positive for anti-Tg antibodies that interfere with the Tg measurement. To overcome this problem, in the present study, we have used absorption spectroscopy technique for the quantification of Tg and TgAb. As a pilot study, current work was carried out on a limited number of 15 serum samples (5 normal and 10 DTC patients) and their absorption spectra were recorded in the UV-VIS region (200 nm-800 nm). Two peaks correspond to immunoglobulin antibody (IgG) were identified in the absorption spectrum of patient's serum sample and the areas/band sums (FWHM) of these two peaks were used to quantify the level of TgAb. The measured values of TgAb by enzyme-linked immunosorbent assay (ELISA) were matching well with the band sums of the IgG peaks proving the good sensitivity of the absorption technique.

AB - Thyroglobulin (Tg) is a glycoprotein specifically synthesized by follicular thyroid epithelium. After thyroidectomy, serum Tg is a specific and sensitive marker for the presence of functioning thyroid tissue, and its measurement is fundamental in the follow-up of patients affected by differentiated thyroid carcinomas (DTCs). Serum antibodies against thyroglobulin (TgAbs) are common in patients with differentiated thyroid cancer (DTC) and can interfere in thyroglobulin (Tg) assays. Unfortunately, serum Tg measurement becomes useless in approximately 10-20% of DTC cases who are positive for anti-Tg antibodies that interfere with the Tg measurement. To overcome this problem, in the present study, we have used absorption spectroscopy technique for the quantification of Tg and TgAb. As a pilot study, current work was carried out on a limited number of 15 serum samples (5 normal and 10 DTC patients) and their absorption spectra were recorded in the UV-VIS region (200 nm-800 nm). Two peaks correspond to immunoglobulin antibody (IgG) were identified in the absorption spectrum of patient's serum sample and the areas/band sums (FWHM) of these two peaks were used to quantify the level of TgAb. The measured values of TgAb by enzyme-linked immunosorbent assay (ELISA) were matching well with the band sums of the IgG peaks proving the good sensitivity of the absorption technique.

UR - http://www.scopus.com/inward/record.url?scp=84896960850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896960850&partnerID=8YFLogxK

U2 - 10.1166/jmihi.2014.1252

DO - 10.1166/jmihi.2014.1252

M3 - Article

VL - 4

SP - 262

EP - 266

JO - Journal of Medical Imaging and Health Informatics

JF - Journal of Medical Imaging and Health Informatics

SN - 2156-7018

IS - 2

ER -