Achieving asepsis of banana leaves for the management of toxic epidermal necrolysis

C. Srinivas, V. Sundaram, B. Raju, S. Prabhu, M. Thirumurthy, A. Bhaskar

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Banana leaf is used in many centers in India during the care of patients with toxic epidermal necrolysis (TEN) and other extensive blistering disorders. Sepsis is an important cause of death in TEN patients and use of banana leaf may be a source of such infection. Aims: We conducted this study to detect the bacterial flora of the banana leaf and to examine various methods of rendering the leaf aseptic. Methods: Five pieces of banana leaf, 2 x 2 cm in size, were cultured separately in blood agar as follows: One piece was heated over a flame and one was soaked in boiling water and one was autoclaved. Methylated spirit was applied over one piece and ignited. One piece was placed on the media, ′as is.′ The Petri dishes were incubated examined after 48 h. Results: All the pieces except the autoclaved specimen of the leaf grew coagulase-negative staphylococci (CONS) when aseptic precautions were not maintained and aerobic spore bearers when all aseptic measures were subsequently instituted during the procedure. Conclusion: We recommend measures to prevent possible transmission of bacterial infection by the leaf. Autoclaved and aseptically handled banana leaves may be used to reduce chance of infection in the treatment of TEN.

Original languageEnglish
Pages (from-to)201-202
Number of pages2
JournalIndian Journal of Dermatology, Venereology and Leprology
Volume72
Issue number3
Publication statusPublished - 01-05-2006

Fingerprint

Asepsis
Stevens-Johnson Syndrome
Musa
Coagulase
Infection
Spores
Staphylococcus
Bacterial Infections
Agar
India
Cause of Death
Sepsis
Patient Care
Water

All Science Journal Classification (ASJC) codes

  • Dermatology
  • Infectious Diseases

Cite this

Srinivas, C. ; Sundaram, V. ; Raju, B. ; Prabhu, S. ; Thirumurthy, M. ; Bhaskar, A. / Achieving asepsis of banana leaves for the management of toxic epidermal necrolysis. In: Indian Journal of Dermatology, Venereology and Leprology. 2006 ; Vol. 72, No. 3. pp. 201-202.
@article{939dc8cfaa954326b30759747b539259,
title = "Achieving asepsis of banana leaves for the management of toxic epidermal necrolysis",
abstract = "Background: Banana leaf is used in many centers in India during the care of patients with toxic epidermal necrolysis (TEN) and other extensive blistering disorders. Sepsis is an important cause of death in TEN patients and use of banana leaf may be a source of such infection. Aims: We conducted this study to detect the bacterial flora of the banana leaf and to examine various methods of rendering the leaf aseptic. Methods: Five pieces of banana leaf, 2 x 2 cm in size, were cultured separately in blood agar as follows: One piece was heated over a flame and one was soaked in boiling water and one was autoclaved. Methylated spirit was applied over one piece and ignited. One piece was placed on the media, ′as is.′ The Petri dishes were incubated examined after 48 h. Results: All the pieces except the autoclaved specimen of the leaf grew coagulase-negative staphylococci (CONS) when aseptic precautions were not maintained and aerobic spore bearers when all aseptic measures were subsequently instituted during the procedure. Conclusion: We recommend measures to prevent possible transmission of bacterial infection by the leaf. Autoclaved and aseptically handled banana leaves may be used to reduce chance of infection in the treatment of TEN.",
author = "C. Srinivas and V. Sundaram and B. Raju and S. Prabhu and M. Thirumurthy and A. Bhaskar",
year = "2006",
month = "5",
day = "1",
language = "English",
volume = "72",
pages = "201--202",
journal = "Indian Journal of Dermatology, Venereology and Leprology",
issn = "0378-6323",
publisher = "Medknow Publications and Media Pvt. Ltd",
number = "3",

}

Srinivas, C, Sundaram, V, Raju, B, Prabhu, S, Thirumurthy, M & Bhaskar, A 2006, 'Achieving asepsis of banana leaves for the management of toxic epidermal necrolysis', Indian Journal of Dermatology, Venereology and Leprology, vol. 72, no. 3, pp. 201-202.

Achieving asepsis of banana leaves for the management of toxic epidermal necrolysis. / Srinivas, C.; Sundaram, V.; Raju, B.; Prabhu, S.; Thirumurthy, M.; Bhaskar, A.

In: Indian Journal of Dermatology, Venereology and Leprology, Vol. 72, No. 3, 01.05.2006, p. 201-202.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Achieving asepsis of banana leaves for the management of toxic epidermal necrolysis

AU - Srinivas, C.

AU - Sundaram, V.

AU - Raju, B.

AU - Prabhu, S.

AU - Thirumurthy, M.

AU - Bhaskar, A.

PY - 2006/5/1

Y1 - 2006/5/1

N2 - Background: Banana leaf is used in many centers in India during the care of patients with toxic epidermal necrolysis (TEN) and other extensive blistering disorders. Sepsis is an important cause of death in TEN patients and use of banana leaf may be a source of such infection. Aims: We conducted this study to detect the bacterial flora of the banana leaf and to examine various methods of rendering the leaf aseptic. Methods: Five pieces of banana leaf, 2 x 2 cm in size, were cultured separately in blood agar as follows: One piece was heated over a flame and one was soaked in boiling water and one was autoclaved. Methylated spirit was applied over one piece and ignited. One piece was placed on the media, ′as is.′ The Petri dishes were incubated examined after 48 h. Results: All the pieces except the autoclaved specimen of the leaf grew coagulase-negative staphylococci (CONS) when aseptic precautions were not maintained and aerobic spore bearers when all aseptic measures were subsequently instituted during the procedure. Conclusion: We recommend measures to prevent possible transmission of bacterial infection by the leaf. Autoclaved and aseptically handled banana leaves may be used to reduce chance of infection in the treatment of TEN.

AB - Background: Banana leaf is used in many centers in India during the care of patients with toxic epidermal necrolysis (TEN) and other extensive blistering disorders. Sepsis is an important cause of death in TEN patients and use of banana leaf may be a source of such infection. Aims: We conducted this study to detect the bacterial flora of the banana leaf and to examine various methods of rendering the leaf aseptic. Methods: Five pieces of banana leaf, 2 x 2 cm in size, were cultured separately in blood agar as follows: One piece was heated over a flame and one was soaked in boiling water and one was autoclaved. Methylated spirit was applied over one piece and ignited. One piece was placed on the media, ′as is.′ The Petri dishes were incubated examined after 48 h. Results: All the pieces except the autoclaved specimen of the leaf grew coagulase-negative staphylococci (CONS) when aseptic precautions were not maintained and aerobic spore bearers when all aseptic measures were subsequently instituted during the procedure. Conclusion: We recommend measures to prevent possible transmission of bacterial infection by the leaf. Autoclaved and aseptically handled banana leaves may be used to reduce chance of infection in the treatment of TEN.

UR - http://www.scopus.com/inward/record.url?scp=33745143275&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745143275&partnerID=8YFLogxK

M3 - Article

VL - 72

SP - 201

EP - 202

JO - Indian Journal of Dermatology, Venereology and Leprology

JF - Indian Journal of Dermatology, Venereology and Leprology

SN - 0378-6323

IS - 3

ER -