Abstract

The current report intended to inspect the photobiomodulatory effects of 632.8 nm laser on cellular parameters on artificially injured human monolayer skin fibroblast cells growing in vitro. To accomplish the specified objectives, a scratch (1-2 mm) was generated on monolayered human skin fibroblast cells. Further, cells were illuminated with a single dose of 632.8 nm (He-Ne laser; 1.5 cm beam spot; 11.8mW power; 6.68 mWcm-2) at 5 and 7 Jcm-2. Alterations due to red light laser irradiation were appraised by quantifying cytochrome c oxidase activity and Adenosine triphosphate synthesis in wounded skin fibroblast cells at various time points. Elevated ATP synthesis via cytochrome c oxidase activity was noticed in wounded cells following single exposure of 632.8 nm at 5 Jcm-2. In a nutshell, the low level laser therapy boosts numerous cellular processes in wounded fibroblast cells culminating in enhanced tissue repair.

Original languageEnglish
Title of host publicationMedical Laser Applications and Laser-Tissue Interactions IX
EditorsLothar D. Lilge, Carsten M. Philipp
PublisherSPIE
ISBN (Electronic)9781510628519
DOIs
Publication statusPublished - 01-01-2019
EventMedical Laser Applications and Laser-Tissue Interactions IX 2019 - Munich, Germany
Duration: 23-06-201924-06-2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11079
ISSN (Print)1605-7422

Conference

ConferenceMedical Laser Applications and Laser-Tissue Interactions IX 2019
CountryGermany
CityMunich
Period23-06-1924-06-19

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Prabhu, V., Rao, B. S. S., & Mahato, K. K. (2019). Action of He-Ne laser on wounded human skin fibroblast cells. In L. D. Lilge, & C. M. Philipp (Eds.), Medical Laser Applications and Laser-Tissue Interactions IX [110790T] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11079). SPIE. https://doi.org/10.1117/12.2526959