Action of Portulaca oleracea against streptozotocin-induced oxidative stress in experimental diabetic rats

A. Sharma, M. Vijayakumar, Ch.V. Rao, M.K. Unnikrishnan, G.D. Reddy

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Antidiabetic treatment with an extract of Portulaca oleracea leaves (100mg/kg and 250mg/kg body weight) for three weeks showed a significant reduction in thiobarbituric acid reactive substances (TBRAS) and an increase in glutathione reductase (GSH-R) in both the liver and kidney of STZ diabetic rats. The treatment with P. oleracea significantly altered the glutathione and GSH-R to be comparable with the control group. P. oleracea- and tolbutamide-treated rats showed decreased lipid peroxidation that is associated with increased activity of superoxide dismutase (SOD) and catalase(CAT). The effects of P. oleracea on tissue lipid peroxidation and antioxidant status in diabetic animals have not been studied before. The result of this study thus shows that although P. oleracea extract possesses moderate antidiabetic activity, it exhibits potent antioxidant potential in diabetic conditions. Copyright ©2009 The Berkeley Electronic Press. All rights reserved.
Original languageEnglish
JournalJournal of Complementary and Integrative Medicine
Volume6
Issue number1
DOIs
Publication statusPublished - 2009

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Portulaca
Streptozocin
Oxidative Stress
Hypoglycemic Agents
Lipid Peroxidation
Antioxidants
Tolbutamide
Thiobarbituric Acid Reactive Substances
Glutathione Reductase
Catalase
Superoxide Dismutase
Glutathione
Body Weight
Kidney
Control Groups
Liver

Cite this

Sharma, A. ; Vijayakumar, M. ; Rao, Ch.V. ; Unnikrishnan, M.K. ; Reddy, G.D. / Action of Portulaca oleracea against streptozotocin-induced oxidative stress in experimental diabetic rats. In: Journal of Complementary and Integrative Medicine. 2009 ; Vol. 6, No. 1.
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title = "Action of Portulaca oleracea against streptozotocin-induced oxidative stress in experimental diabetic rats",
abstract = "Antidiabetic treatment with an extract of Portulaca oleracea leaves (100mg/kg and 250mg/kg body weight) for three weeks showed a significant reduction in thiobarbituric acid reactive substances (TBRAS) and an increase in glutathione reductase (GSH-R) in both the liver and kidney of STZ diabetic rats. The treatment with P. oleracea significantly altered the glutathione and GSH-R to be comparable with the control group. P. oleracea- and tolbutamide-treated rats showed decreased lipid peroxidation that is associated with increased activity of superoxide dismutase (SOD) and catalase(CAT). The effects of P. oleracea on tissue lipid peroxidation and antioxidant status in diabetic animals have not been studied before. The result of this study thus shows that although P. oleracea extract possesses moderate antidiabetic activity, it exhibits potent antioxidant potential in diabetic conditions. Copyright {\circledC}2009 The Berkeley Electronic Press. All rights reserved.",
author = "A. Sharma and M. Vijayakumar and Ch.V. Rao and M.K. Unnikrishnan and G.D. Reddy",
note = "Cited By :8 Export Date: 10 November 2017 Correspondence Address: Rao, Ch. V.; Pharmacognosy and Ethno Pharmacology Division, National Botanical Research Institute, Lucknow, Uttar Pradesh- 226 001, India; email: amreshgupta@rediffmail.com Chemicals/CAS: catalase, 9001-05-2; glutathione reductase, 9001-48-3; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; tolbutamide, 473-41-6, 64-77-7 References: Aebi, H., Catalase (1983) Methods in enzymatic analysis, pp. 276-286. , Bergmeyer HU ed, Academic Press, New York; Awad, N.E., Lipid content and antimicrobial activity of phenolic constituents of cultivated Portulaca oleracea L (1994) Bull. Fac. Pharm. Cairo Univ, 32, pp. 137-142; Baynes, J.W., Perspectives in diabetes, role of oxidative stress on development of complications in diabetes (1991) Diabetes, 40, pp. 405-412; Beutler, E., Duron, O., Kelly, B.M., Improved method for determination of blood glutathione (1967) J.Lab. Clin. Med, 61, pp. 882-888; Chan, K., Islam, M.W., Kamil, M., Radhakrishnan, R., Zakaria, M.N.M., Habibullah, M., Attas, A., The analgesic and anti-inflammatory effects of Portulaca oleracea L. subsp. sativa (Haw.) Celak (2000) J. Ethnopharmacol, 73, pp. 445-451; Grankvist, K., Markuld, S., Teljedal, I.B., Superoxide dismutase is prophylactic against alloxan diabetes (1981) Nature, 294, pp. 158-161; Horn, H.D., Glutathione reductase (1963) Methods in enzymatic analysis, pp. 875-879. , Bergmyer, H.U, ed, Academic press; Hunt, J.B., Smith, C.C.T., Wolf, S.P., Auto-oxidative glycosylation and possible involvement of peroxides and free radicals in LDL modification by glucose (1990) Diabetes, 39, pp. 420-1424; Illing, E.K.B., Gray, C.H., Lawrence, R.D., Blood glutathione and non glucose reducing substances in diabetes (1991) Biochemical. J, 48, p. 637; Jamall, I.S., Smith, J.C., Effect of cadmium on glutathione peroxides, super oxide dismutase and lipid peroxidation in the rat heart: A possible mechanism of cadmium cardiotoxicity (1985) Toxicol. Appli. Pharmacol, 80, pp. 33-42; Kakkar, P., Das, B., Viswanathan, P.N., Modified spectrophotometric assay of SOD (1984) Indian J. Biochem Biophys, 2, pp. 130-132; Kamalakkanan, N., Prince, P.S.M., Antihyperglycaemic and antioxidant effect of rutin, a polyphenolic flavonoid, in streptozotocin induced diabetic Wister rats (2006) Basic Clin. Pharmacol. Toxicol, 98, pp. 97-103; Karimi1 G, Hosseinzadeh H, Ettehad N. 2004. Evaluation of the gastric Antiulcerogenic effects of Portulaca oleracea L. Extracts in Mice. Phytother. Res. 18:484-487; Karpen, C.W., Pritchard, K.A., Merola, A.J., Ranganamala, R.V., Alterations of the prostaglandin-thromboxin ratio in streptozotocin induced diabetes rats (1982) Prostagland. Leukotrien. Med, 8, pp. 93-103; Liu, L.X., Howe, P.Y., Zhou, F., Xu, Z.Q., Hocart, C., Zhang, R., Fatty acids and β-carotene in Australian purslane (Portulaca oleracea) varieties (2000) J. Chromatogr. A, 893, pp. 207-213; Loven, D., Schedl, H., Wilson, H., Daabees, T.T., Stegink, L.D., Diekus, M., Effect of insulin and oral glutathione on glutathione levels and superoxide dismutase activities in organs of rats with streptozotocin induced (1986) Diabetes, 35, pp. 503-507; McCrod, J.M., Keel, B.B., Fridovich, I., An enzyme based theory of obligate anaerobiosis, the physiological functions of superoxide dismutase (1976) Proc. Natl. Acad.Sci, 68, pp. 1024-1027; Mukherjee, B., Mukherjee, J.H., Chatterjee, M., Lipid peroxidation, gluthione levels and change in gluthione related enzymes activities in streptozotocin-induced diabetic rats (1994) Immunol. Cell. Biol, 72, pp. 109-111; Nadkarni, K.M., Nadkarni, A.K., (1995) Indian Materia Medica, p. 669. , Popular Pakashan, New Delhi; Nakakimura, H., Mizuno, K., Studies on lipid per oxidation in biological system II. Hyperlipoperoxidemia in mice induced by alloxan (1980) Chem. pharm Bull, 28, pp. 2207-2211; Okhawa, H., Oshishi, N., Yagi, K., Assay for lipid peroxised in animals tissues by thiobarbituric acid reaction (1979) Anal. Biochem, 95, pp. 351-355; Parry, O., Okwuasaba, F., Ejike, C., Skeletal muscle relaxant action of an aqueous extract of Portulaca oleracea in the rat (1987) J. Ethnopharmacol, 19, pp. 247-253; Parry, O., Marks, J.A., Okwuasaba, F., The skeletal muscle relaxant action of Portulaca oleracea: Role of potassium ions (1993) J. Ethnopharmacol, 40, pp. 187-194; Rashed, N., Afifi, F.U., Disi, A.M., Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI-1 (2003) J. Ethnopharmacol, 88, pp. 131-136; Rauscher, F.M., Sanders, R.A., Watkins, J.B., Effects of new antioxidant compounds PNU-104067F and PNU-74389G on antioxidant defence in normal and diabetic rats J. Biochem. Mol.Toxicol, 14, pp. 189-194; Sakai, N., Inada, K., Okamoto, M., Shizuri, Y., Fukuyama, Y., Portuloside-A, a monoterpene glucoside from Portulaca oleracea (1996) Phytochem, 42, pp. 1625-1628; Santhakumari, P., Prakasam, A., Pugalendi, K.V., Modulation of oxidative stress parameters by treatment with piper betel leaf in streptozotocin induced diabetic rats (2003) Indian J. Pharmacol, 35, pp. 373-378; Simopoulos, A.P., Salem, H.J., Purslane: A terrestrial source of omega-3 fatty acids (1986) N. Engl. J. Med, 315, p. 833; Vijayakumar, M., Govindarajan, R., Rao, G.M.M., Rao, C.V., Shirwaikar, A., Mehrotra, S., Pushpangadan, P., Action of Hygrophila auriculata against streptozotocin induced oxidative stress (2006) J. Ethnopharmacol, 104, pp. 356-361; (2004) Diabetes action Now: An Initiative of the World Health Organization and International Diabetes Federation, , World Health Organization, WHO Publication, Geneva; Xiang, L., Xing, D., Wang, W., Wang, F., Ding, Y., Du, L., Alkaloids from Portulaca oleracea L (2005) Phytochem, 66, pp. 2595-2601; Zhang XJ, Ji YB, Qu ZhY, Xia JCh, Wang L. 2002. Experimental studies on antibiotic functions of Portulaca oleracea L. in vitro. Chinese J. Microecol. 14:277-280UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-62549126003&doi=10.2202{\%}2f1553-3840.1181&partnerID=40&md5=9165ba952d10106b7b88963677e45112",
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Action of Portulaca oleracea against streptozotocin-induced oxidative stress in experimental diabetic rats. / Sharma, A.; Vijayakumar, M.; Rao, Ch.V.; Unnikrishnan, M.K.; Reddy, G.D.

In: Journal of Complementary and Integrative Medicine, Vol. 6, No. 1, 2009.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Action of Portulaca oleracea against streptozotocin-induced oxidative stress in experimental diabetic rats

AU - Sharma, A.

AU - Vijayakumar, M.

AU - Rao, Ch.V.

AU - Unnikrishnan, M.K.

AU - Reddy, G.D.

N1 - Cited By :8 Export Date: 10 November 2017 Correspondence Address: Rao, Ch. V.; Pharmacognosy and Ethno Pharmacology Division, National Botanical Research Institute, Lucknow, Uttar Pradesh- 226 001, India; email: amreshgupta@rediffmail.com Chemicals/CAS: catalase, 9001-05-2; glutathione reductase, 9001-48-3; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; tolbutamide, 473-41-6, 64-77-7 References: Aebi, H., Catalase (1983) Methods in enzymatic analysis, pp. 276-286. , Bergmeyer HU ed, Academic Press, New York; Awad, N.E., Lipid content and antimicrobial activity of phenolic constituents of cultivated Portulaca oleracea L (1994) Bull. Fac. Pharm. Cairo Univ, 32, pp. 137-142; Baynes, J.W., Perspectives in diabetes, role of oxidative stress on development of complications in diabetes (1991) Diabetes, 40, pp. 405-412; Beutler, E., Duron, O., Kelly, B.M., Improved method for determination of blood glutathione (1967) J.Lab. Clin. Med, 61, pp. 882-888; Chan, K., Islam, M.W., Kamil, M., Radhakrishnan, R., Zakaria, M.N.M., Habibullah, M., Attas, A., The analgesic and anti-inflammatory effects of Portulaca oleracea L. subsp. sativa (Haw.) Celak (2000) J. Ethnopharmacol, 73, pp. 445-451; Grankvist, K., Markuld, S., Teljedal, I.B., Superoxide dismutase is prophylactic against alloxan diabetes (1981) Nature, 294, pp. 158-161; Horn, H.D., Glutathione reductase (1963) Methods in enzymatic analysis, pp. 875-879. , Bergmyer, H.U, ed, Academic press; Hunt, J.B., Smith, C.C.T., Wolf, S.P., Auto-oxidative glycosylation and possible involvement of peroxides and free radicals in LDL modification by glucose (1990) Diabetes, 39, pp. 420-1424; Illing, E.K.B., Gray, C.H., Lawrence, R.D., Blood glutathione and non glucose reducing substances in diabetes (1991) Biochemical. J, 48, p. 637; Jamall, I.S., Smith, J.C., Effect of cadmium on glutathione peroxides, super oxide dismutase and lipid peroxidation in the rat heart: A possible mechanism of cadmium cardiotoxicity (1985) Toxicol. Appli. Pharmacol, 80, pp. 33-42; Kakkar, P., Das, B., Viswanathan, P.N., Modified spectrophotometric assay of SOD (1984) Indian J. Biochem Biophys, 2, pp. 130-132; Kamalakkanan, N., Prince, P.S.M., Antihyperglycaemic and antioxidant effect of rutin, a polyphenolic flavonoid, in streptozotocin induced diabetic Wister rats (2006) Basic Clin. Pharmacol. Toxicol, 98, pp. 97-103; Karimi1 G, Hosseinzadeh H, Ettehad N. 2004. Evaluation of the gastric Antiulcerogenic effects of Portulaca oleracea L. Extracts in Mice. Phytother. Res. 18:484-487; Karpen, C.W., Pritchard, K.A., Merola, A.J., Ranganamala, R.V., Alterations of the prostaglandin-thromboxin ratio in streptozotocin induced diabetes rats (1982) Prostagland. Leukotrien. Med, 8, pp. 93-103; Liu, L.X., Howe, P.Y., Zhou, F., Xu, Z.Q., Hocart, C., Zhang, R., Fatty acids and β-carotene in Australian purslane (Portulaca oleracea) varieties (2000) J. Chromatogr. A, 893, pp. 207-213; Loven, D., Schedl, H., Wilson, H., Daabees, T.T., Stegink, L.D., Diekus, M., Effect of insulin and oral glutathione on glutathione levels and superoxide dismutase activities in organs of rats with streptozotocin induced (1986) Diabetes, 35, pp. 503-507; McCrod, J.M., Keel, B.B., Fridovich, I., An enzyme based theory of obligate anaerobiosis, the physiological functions of superoxide dismutase (1976) Proc. Natl. Acad.Sci, 68, pp. 1024-1027; Mukherjee, B., Mukherjee, J.H., Chatterjee, M., Lipid peroxidation, gluthione levels and change in gluthione related enzymes activities in streptozotocin-induced diabetic rats (1994) Immunol. Cell. Biol, 72, pp. 109-111; Nadkarni, K.M., Nadkarni, A.K., (1995) Indian Materia Medica, p. 669. , Popular Pakashan, New Delhi; Nakakimura, H., Mizuno, K., Studies on lipid per oxidation in biological system II. Hyperlipoperoxidemia in mice induced by alloxan (1980) Chem. pharm Bull, 28, pp. 2207-2211; Okhawa, H., Oshishi, N., Yagi, K., Assay for lipid peroxised in animals tissues by thiobarbituric acid reaction (1979) Anal. Biochem, 95, pp. 351-355; Parry, O., Okwuasaba, F., Ejike, C., Skeletal muscle relaxant action of an aqueous extract of Portulaca oleracea in the rat (1987) J. Ethnopharmacol, 19, pp. 247-253; Parry, O., Marks, J.A., Okwuasaba, F., The skeletal muscle relaxant action of Portulaca oleracea: Role of potassium ions (1993) J. Ethnopharmacol, 40, pp. 187-194; Rashed, N., Afifi, F.U., Disi, A.M., Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI-1 (2003) J. Ethnopharmacol, 88, pp. 131-136; Rauscher, F.M., Sanders, R.A., Watkins, J.B., Effects of new antioxidant compounds PNU-104067F and PNU-74389G on antioxidant defence in normal and diabetic rats J. Biochem. Mol.Toxicol, 14, pp. 189-194; Sakai, N., Inada, K., Okamoto, M., Shizuri, Y., Fukuyama, Y., Portuloside-A, a monoterpene glucoside from Portulaca oleracea (1996) Phytochem, 42, pp. 1625-1628; Santhakumari, P., Prakasam, A., Pugalendi, K.V., Modulation of oxidative stress parameters by treatment with piper betel leaf in streptozotocin induced diabetic rats (2003) Indian J. Pharmacol, 35, pp. 373-378; Simopoulos, A.P., Salem, H.J., Purslane: A terrestrial source of omega-3 fatty acids (1986) N. Engl. J. Med, 315, p. 833; Vijayakumar, M., Govindarajan, R., Rao, G.M.M., Rao, C.V., Shirwaikar, A., Mehrotra, S., Pushpangadan, P., Action of Hygrophila auriculata against streptozotocin induced oxidative stress (2006) J. Ethnopharmacol, 104, pp. 356-361; (2004) Diabetes action Now: An Initiative of the World Health Organization and International Diabetes Federation, , World Health Organization, WHO Publication, Geneva; Xiang, L., Xing, D., Wang, W., Wang, F., Ding, Y., Du, L., Alkaloids from Portulaca oleracea L (2005) Phytochem, 66, pp. 2595-2601; Zhang XJ, Ji YB, Qu ZhY, Xia JCh, Wang L. 2002. Experimental studies on antibiotic functions of Portulaca oleracea L. in vitro. Chinese J. Microecol. 14:277-280UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-62549126003&doi=10.2202%2f1553-3840.1181&partnerID=40&md5=9165ba952d10106b7b88963677e45112

PY - 2009

Y1 - 2009

N2 - Antidiabetic treatment with an extract of Portulaca oleracea leaves (100mg/kg and 250mg/kg body weight) for three weeks showed a significant reduction in thiobarbituric acid reactive substances (TBRAS) and an increase in glutathione reductase (GSH-R) in both the liver and kidney of STZ diabetic rats. The treatment with P. oleracea significantly altered the glutathione and GSH-R to be comparable with the control group. P. oleracea- and tolbutamide-treated rats showed decreased lipid peroxidation that is associated with increased activity of superoxide dismutase (SOD) and catalase(CAT). The effects of P. oleracea on tissue lipid peroxidation and antioxidant status in diabetic animals have not been studied before. The result of this study thus shows that although P. oleracea extract possesses moderate antidiabetic activity, it exhibits potent antioxidant potential in diabetic conditions. Copyright ©2009 The Berkeley Electronic Press. All rights reserved.

AB - Antidiabetic treatment with an extract of Portulaca oleracea leaves (100mg/kg and 250mg/kg body weight) for three weeks showed a significant reduction in thiobarbituric acid reactive substances (TBRAS) and an increase in glutathione reductase (GSH-R) in both the liver and kidney of STZ diabetic rats. The treatment with P. oleracea significantly altered the glutathione and GSH-R to be comparable with the control group. P. oleracea- and tolbutamide-treated rats showed decreased lipid peroxidation that is associated with increased activity of superoxide dismutase (SOD) and catalase(CAT). The effects of P. oleracea on tissue lipid peroxidation and antioxidant status in diabetic animals have not been studied before. The result of this study thus shows that although P. oleracea extract possesses moderate antidiabetic activity, it exhibits potent antioxidant potential in diabetic conditions. Copyright ©2009 The Berkeley Electronic Press. All rights reserved.

U2 - 10.2202/1553-3840.1181

DO - 10.2202/1553-3840.1181

M3 - Article

VL - 6

JO - Journal of Complementary and Integrative Medicine

JF - Journal of Complementary and Integrative Medicine

SN - 1553-3840

IS - 1

ER -