Actions of antidiuretic hormone analogues on intact and nystatin-permeabilized frog skins: Experimental Physiology-Research Paper

Silviya Rajakumari Jared, J. Prakasa Rao, Sathya Subramani

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The roles of two antidiuretic hormone analogues, namely arginine vasotocin (AVT) and lysine vasopressin (LVP), in solute transport across the ventral abdominal skin of frogs (Rana hexadactyla) were studied using voltage-clamp methods on intact and nystatin-permeabilized preparations. Arginine vasotocin (40 nm), the amphibian analogue of antidiuretic hormone, did not have any effect on the skin of Rana hexadactyla. However, LVP, the porcine antidiuretic hormone, increased the transepithelial potential difference (TEPD) and short-circuit current (SCC) significantly, without affecting the slope conductance. Lysine vasopressin had no action subsequent to addition of amiloride (100 μm) on the apical side or ouabain (10 μm) on the basolateral side. Lysine vasopressin increased slope conductance in the nystatin-permeablized skin while decreasing TEPD. Such a change was not seen in chloride-free solutions. To elucidate the mechanism of action of LVP on intact skin, experiments were done with forskolin and a V2 receptor blocker. The effects of forskolin (10 μm) were different from those of LVP in that forskolin significantly increased SCC and conductance of the intact skin, while decreasing TEPD. The forskolin-induced increase in conductance was not abolished by amiloride. Use of the V2 receptor blocker inhibited the effects of LVP. We conclude that AVT does not have an action on the skin of Rana hexadactyla. Lysine vasopressin enhances transepithelial sodium transport by increasing sodium-potassium pump activity, while not affecting the epithelial sodium channel conductance. Lysine vasopressin also enhances an inward-directed conductance on the basolateral membrane, probably a chloride conductance. The action of LVP on the intact frog skin is through the V2 receptors; however, downstream signalling does not seem to be mediated by cAMP. Analysis of the electrophysiological model of frog skin with LVP allows us additionally to conclude that modulation of channel activity and not carrier-mediated transport affects slope conductance.

Original languageEnglish
Pages (from-to)1174-1184
Number of pages11
JournalExperimental Physiology
Volume94
Issue number12
DOIs
Publication statusPublished - 01-12-2009
Externally publishedYes

Fingerprint

Skin Physiological Phenomena
Lypressin
Nystatin
Vasopressins
Anura
Research
Skin
Colforsin
Vasotocin
Ranidae
Vasopressin Receptors
Amiloride
Chlorides
Epithelial Sodium Channels
Sodium-Potassium-Exchanging ATPase
Arginine Vasopressin
Amphibians
Ouabain

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Jared, Silviya Rajakumari ; Rao, J. Prakasa ; Subramani, Sathya. / Actions of antidiuretic hormone analogues on intact and nystatin-permeabilized frog skins : Experimental Physiology-Research Paper. In: Experimental Physiology. 2009 ; Vol. 94, No. 12. pp. 1174-1184.
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Actions of antidiuretic hormone analogues on intact and nystatin-permeabilized frog skins : Experimental Physiology-Research Paper. / Jared, Silviya Rajakumari; Rao, J. Prakasa; Subramani, Sathya.

In: Experimental Physiology, Vol. 94, No. 12, 01.12.2009, p. 1174-1184.

Research output: Contribution to journalArticle

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