Activated endothelial cells induce neutrophil extracellular traps and are susceptible to NETosis-mediated cell death

Anurag Kumar Gupta, Manjunath B. Joshi, Maria Philippova, Paul Erne, Paul Hasler, Sinuhe Hahn, Therese J. Resink

Research output: Contribution to journalArticle

237 Citations (Scopus)

Abstract

Neutrophil interaction with activated endothelial cells (EC) is required for transmigration. We examined consequences of this interaction on NETosis. Co-culture of activated EC with neutrophils induced neutrophil extracellular trap (NET) formation, which was partially dependent on production of IL-8 by activated EC. Extended neutophil/EC co-culture resulted in EC damage, which could be abrogated by inclusion of either diphenyleneiodonium to inhibit the NAPDH oxidase pathway required for NETosis, or DNAse to disrupt NETs. These findings offer new insight into mechanisms whereby NETs trigger damage to the endothelium in sepsis, small vessel vasculitis and possibly the villous trophoblast in preeclampsia.

Original languageEnglish
Pages (from-to)3193-3197
Number of pages5
JournalFEBS Letters
Volume584
Issue number14
DOIs
Publication statusPublished - 07-2010

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

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