Activated endothelial cells induce neutrophil extracellular traps and are susceptible to NETosis-mediated cell death

Anurag Kumar Gupta, Manjunath B. Joshi, Maria Philippova, Paul Erne, Paul Hasler, Sinuhe Hahn, Therese J. Resink

    Research output: Contribution to journalArticle

    207 Citations (Scopus)

    Abstract

    Neutrophil interaction with activated endothelial cells (EC) is required for transmigration. We examined consequences of this interaction on NETosis. Co-culture of activated EC with neutrophils induced neutrophil extracellular trap (NET) formation, which was partially dependent on production of IL-8 by activated EC. Extended neutophil/EC co-culture resulted in EC damage, which could be abrogated by inclusion of either diphenyleneiodonium to inhibit the NAPDH oxidase pathway required for NETosis, or DNAse to disrupt NETs. These findings offer new insight into mechanisms whereby NETs trigger damage to the endothelium in sepsis, small vessel vasculitis and possibly the villous trophoblast in preeclampsia.

    Original languageEnglish
    Pages (from-to)3193-3197
    Number of pages5
    JournalFEBS Letters
    Volume584
    Issue number14
    DOIs
    Publication statusPublished - 07-2010

    Fingerprint

    Endothelial cells
    Cell death
    Cell Death
    Endothelial Cells
    Coculture Techniques
    Cell culture
    Neutrophils
    Trophoblasts
    Vasculitis
    Pre-Eclampsia
    Interleukin-8
    Endothelium
    Sepsis
    Oxidoreductases
    Cell Culture Techniques
    Extracellular Traps

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Biophysics
    • Cell Biology
    • Genetics
    • Molecular Biology
    • Structural Biology

    Cite this

    Gupta, Anurag Kumar ; Joshi, Manjunath B. ; Philippova, Maria ; Erne, Paul ; Hasler, Paul ; Hahn, Sinuhe ; Resink, Therese J. / Activated endothelial cells induce neutrophil extracellular traps and are susceptible to NETosis-mediated cell death. In: FEBS Letters. 2010 ; Vol. 584, No. 14. pp. 3193-3197.
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    Activated endothelial cells induce neutrophil extracellular traps and are susceptible to NETosis-mediated cell death. / Gupta, Anurag Kumar; Joshi, Manjunath B.; Philippova, Maria; Erne, Paul; Hasler, Paul; Hahn, Sinuhe; Resink, Therese J.

    In: FEBS Letters, Vol. 584, No. 14, 07.2010, p. 3193-3197.

    Research output: Contribution to journalArticle

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    AU - Joshi, Manjunath B.

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    AB - Neutrophil interaction with activated endothelial cells (EC) is required for transmigration. We examined consequences of this interaction on NETosis. Co-culture of activated EC with neutrophils induced neutrophil extracellular trap (NET) formation, which was partially dependent on production of IL-8 by activated EC. Extended neutophil/EC co-culture resulted in EC damage, which could be abrogated by inclusion of either diphenyleneiodonium to inhibit the NAPDH oxidase pathway required for NETosis, or DNAse to disrupt NETs. These findings offer new insight into mechanisms whereby NETs trigger damage to the endothelium in sepsis, small vessel vasculitis and possibly the villous trophoblast in preeclampsia.

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