Activity guided isolation of antioxidant tannoid principles from Anogeissus latifolia

R. Govindarajan, M. Vijayakumar, A. Shirwaikar, A.K.S. Rawat, S. Mehrotra, P. Pushpangadan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Oxidative stress is an important causative factor in several human chronic diseases, such as atherosclerosis, cardiovascular disorders, mutagenesis, cancer, several neurodegenerative disorders, and the aging process. Phenolics and tannins are reported to be good antioxidants. Anogeissus latifolia (Combretaceae) bark has been used in the Indian traditional systems of medicine for curing a variety of ailments, but scientific validation is not available till date. Hence the present study was undertaken to isolate antioxidant compounds by activity-guided isolation. Inhibtion of diphenyl picryl hydrazyl (DPPH) and Xanthine oxidase along with photochemiluminescence assay were used as bioassay for antioxidant activity. Activity guided isolation was carried out using silica column and the compounds were quantified using HPLC. Ethyl acetate and butanol fraction exhibited potent antioxidant activity. Bioassay-guided isolation led to isolation of ellagic acid (1) and dimethyl ellagic acid (2) as the main active compounds, which along with gallic acid were quantified by HPLC. Thus we conclude that these three major tannoid principles present in A. latifolia, are responsible for the antioxidant potential and possibly their therapeutic potential.
Original languageEnglish
Pages (from-to)174-178
Number of pages5
JournalNatural Product Sciences
Volume11
Issue number3
Publication statusPublished - 2005

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Antioxidants
Ellagic Acid
Bioassay
Biological Assay
Combretaceae
High Pressure Liquid Chromatography
Gallic Acid
Mutagenesis
Butanols
Oxidative stress
Xanthine Oxidase
Tannins
Traditional Medicine
Systems Analysis
Silicon Dioxide
Neurodegenerative Diseases
Medicine
Curing
Assays
Atherosclerosis

Cite this

Govindarajan, R., Vijayakumar, M., Shirwaikar, A., Rawat, A. K. S., Mehrotra, S., & Pushpangadan, P. (2005). Activity guided isolation of antioxidant tannoid principles from Anogeissus latifolia. Natural Product Sciences, 11(3), 174-178.
Govindarajan, R. ; Vijayakumar, M. ; Shirwaikar, A. ; Rawat, A.K.S. ; Mehrotra, S. ; Pushpangadan, P. / Activity guided isolation of antioxidant tannoid principles from Anogeissus latifolia. In: Natural Product Sciences. 2005 ; Vol. 11, No. 3. pp. 174-178.
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abstract = "Oxidative stress is an important causative factor in several human chronic diseases, such as atherosclerosis, cardiovascular disorders, mutagenesis, cancer, several neurodegenerative disorders, and the aging process. Phenolics and tannins are reported to be good antioxidants. Anogeissus latifolia (Combretaceae) bark has been used in the Indian traditional systems of medicine for curing a variety of ailments, but scientific validation is not available till date. Hence the present study was undertaken to isolate antioxidant compounds by activity-guided isolation. Inhibtion of diphenyl picryl hydrazyl (DPPH) and Xanthine oxidase along with photochemiluminescence assay were used as bioassay for antioxidant activity. Activity guided isolation was carried out using silica column and the compounds were quantified using HPLC. Ethyl acetate and butanol fraction exhibited potent antioxidant activity. Bioassay-guided isolation led to isolation of ellagic acid (1) and dimethyl ellagic acid (2) as the main active compounds, which along with gallic acid were quantified by HPLC. Thus we conclude that these three major tannoid principles present in A. latifolia, are responsible for the antioxidant potential and possibly their therapeutic potential.",
author = "R. Govindarajan and M. Vijayakumar and A. Shirwaikar and A.K.S. Rawat and S. Mehrotra and P. Pushpangadan",
note = "Cited By :6 Export Date: 10 November 2017 CODEN: NPSCF Correspondence Address: Govindarajan, R.; Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow-226 001, India; email: govind108@yahoo.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; acetic acid ethyl ester, 141-78-6; butanol, 35296-72-1, 71-36-3; ellagic acid, 476-66-4; gallic acid, 149-91-7; silicon dioxide, 10279-57-9, 14464-46-1, 14808-60-7, 15468-32-3, 60676-86-0, 7631-86-9; xanthine oxidase, 9002-17-9 References: Apparanantham, T., Chelladurai, V., Glimpses of folk medicines of Dharmapuri forest division, Tamil Nadu (1986) Anc. Sci. Life., 5, pp. 182-185; Balla, N.P., Sahu, T.R., Mishra, G.P., Traditional plant medicines of Sagar district, Madhya Pradhesh (1982) J. Econ. Taxon. Bot., 3, pp. 23-32; Barch, D.H., Rundhaugen, L.M., Stoner, G.D., Pillay, N.S., Rosche, W.A., Structure function relationships of the dietary anticarcinogen ellagic acid (1996) Carcinogenisis, 17, pp. 265-269; Deshpande, V.H., Patil, A.D., Rama Rao, A.V., Venkatraman, K., 3,3′-Di-O-methylellagic acid-4′-β-D-xyloside and 3,4,3′-tri-O-methylflavellagic acid-4′-β-D-glucoside from Anogeissus latifolia bark (1976) Indian J. Chem., 14 B, pp. 641-643; Govindarajan, R., Vijayakumar, M., Rao, Ch.V., Rawat, A.K.S., Shirwaikar, A., Mehrotra, S., Pushpangadan, P., Antioxidant potential of Anogeissus latifolia (2004) Biol. Pharm. Bull., 27, pp. 1266-1269; Govindarajan, R., Vijayakumar, M., Rao, Ch.V., Shirwaikar, A., Mehrotra, S., Pushpangadan, P., Healing potential of Anogeissus latifolia in dermal wounds (2004) Acta Pharm., 54, pp. 331-338; Gyamfi, M.A., Aniya, Y., Antioxidant properties of Thonningianin A, isolated from the African medicinal herbs, Thonningia sanguinea (2002) Biochem. Pharmacol., 63, pp. 1725-1737; Jain, S.K., Tarafder, C.R., Medicinal plant lore of the Santal. A review of P.O. bedding's work (1970) Econ. Bot., 24, pp. 241-278; Mishra, R., Billore, K.V., Some ethnobotanical lores from Banswara district, Kerala state (1983) Nagarjun, 26, pp. 229-231; Nawwar, M.A.M., Souleman, A.M.A., 3,4,8,9,10-Pentahydroxy-dibenzo[b,d]pyran-6-one from Tamarix nilotica (1984) Phytochemistry, 23, p. 2966; Peach, K., Tracy, M.V., (1955) Modern Methods of Analysis, , Heidelberg, Springer Verlag; Ramachandran, V.S., Nair, N.C., Ethnobotanical studies in Cannanore disctrict, Kerala state (1981) J. Econ. Taxon. Bot., 2, pp. 183-190; Reddy, K.K., Rajadurai, S., Nayudamma, Y., Studies on Dhava (Anogiessus latifolia) tannins: Part III-polyphenols of bark, sapwood and heartwood of Dhava (1965) Indian J. Chem., 3, pp. 308-310; Roy, G.P., Chaturvedi, D.P., Ethnomedicinal trees of Abujh-Marh area, Madhya Pradesh (1986) Folklore, 27, pp. 95-100; Scalbert, A., Monties, B., Favre, J.M., Polyphenols of Quercus robur. adult tree and in votro grown calli and shoots (1988) Phytochemistry, 27, pp. 3483-3488; Schmeda, H.G., Gutierrez, M.I., Loyola, J.I., Zuniga, J., Biological activity and xanthine oxidase inhibitors from Scirpus califormicus (C.A. Mey.) Steud (1996) Phytother. Res., 10, pp. 683-685; Sreejayan, N., Rao, M.N.A., Free radical scavenging activity of curcumnoids (1996) Drug Res., 46, pp. 169-171; Taylor, W.H., Sinha, A., Khan, I.A., McDaniel, S.T., Esko, J.D., Primers of glycosaminoglycan biosynthesis from Peruvian rain forest plants (1998) J. Biol. Chem., 273, pp. 22260-22266; Theismania, K.C., Kuttan, R., Inhibition of liver fibrosis by ellagic acid (1996) Indian J. Physiol. Pharmacol., 40, pp. 363-366",
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Govindarajan, R, Vijayakumar, M, Shirwaikar, A, Rawat, AKS, Mehrotra, S & Pushpangadan, P 2005, 'Activity guided isolation of antioxidant tannoid principles from Anogeissus latifolia', Natural Product Sciences, vol. 11, no. 3, pp. 174-178.

Activity guided isolation of antioxidant tannoid principles from Anogeissus latifolia. / Govindarajan, R.; Vijayakumar, M.; Shirwaikar, A.; Rawat, A.K.S.; Mehrotra, S.; Pushpangadan, P.

In: Natural Product Sciences, Vol. 11, No. 3, 2005, p. 174-178.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Activity guided isolation of antioxidant tannoid principles from Anogeissus latifolia

AU - Govindarajan, R.

AU - Vijayakumar, M.

AU - Shirwaikar, A.

AU - Rawat, A.K.S.

AU - Mehrotra, S.

AU - Pushpangadan, P.

N1 - Cited By :6 Export Date: 10 November 2017 CODEN: NPSCF Correspondence Address: Govindarajan, R.; Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow-226 001, India; email: govind108@yahoo.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; acetic acid ethyl ester, 141-78-6; butanol, 35296-72-1, 71-36-3; ellagic acid, 476-66-4; gallic acid, 149-91-7; silicon dioxide, 10279-57-9, 14464-46-1, 14808-60-7, 15468-32-3, 60676-86-0, 7631-86-9; xanthine oxidase, 9002-17-9 References: Apparanantham, T., Chelladurai, V., Glimpses of folk medicines of Dharmapuri forest division, Tamil Nadu (1986) Anc. Sci. Life., 5, pp. 182-185; Balla, N.P., Sahu, T.R., Mishra, G.P., Traditional plant medicines of Sagar district, Madhya Pradhesh (1982) J. Econ. Taxon. Bot., 3, pp. 23-32; Barch, D.H., Rundhaugen, L.M., Stoner, G.D., Pillay, N.S., Rosche, W.A., Structure function relationships of the dietary anticarcinogen ellagic acid (1996) Carcinogenisis, 17, pp. 265-269; Deshpande, V.H., Patil, A.D., Rama Rao, A.V., Venkatraman, K., 3,3′-Di-O-methylellagic acid-4′-β-D-xyloside and 3,4,3′-tri-O-methylflavellagic acid-4′-β-D-glucoside from Anogeissus latifolia bark (1976) Indian J. Chem., 14 B, pp. 641-643; Govindarajan, R., Vijayakumar, M., Rao, Ch.V., Rawat, A.K.S., Shirwaikar, A., Mehrotra, S., Pushpangadan, P., Antioxidant potential of Anogeissus latifolia (2004) Biol. Pharm. Bull., 27, pp. 1266-1269; Govindarajan, R., Vijayakumar, M., Rao, Ch.V., Shirwaikar, A., Mehrotra, S., Pushpangadan, P., Healing potential of Anogeissus latifolia in dermal wounds (2004) Acta Pharm., 54, pp. 331-338; Gyamfi, M.A., Aniya, Y., Antioxidant properties of Thonningianin A, isolated from the African medicinal herbs, Thonningia sanguinea (2002) Biochem. Pharmacol., 63, pp. 1725-1737; Jain, S.K., Tarafder, C.R., Medicinal plant lore of the Santal. A review of P.O. bedding's work (1970) Econ. Bot., 24, pp. 241-278; Mishra, R., Billore, K.V., Some ethnobotanical lores from Banswara district, Kerala state (1983) Nagarjun, 26, pp. 229-231; Nawwar, M.A.M., Souleman, A.M.A., 3,4,8,9,10-Pentahydroxy-dibenzo[b,d]pyran-6-one from Tamarix nilotica (1984) Phytochemistry, 23, p. 2966; Peach, K., Tracy, M.V., (1955) Modern Methods of Analysis, , Heidelberg, Springer Verlag; Ramachandran, V.S., Nair, N.C., Ethnobotanical studies in Cannanore disctrict, Kerala state (1981) J. Econ. Taxon. Bot., 2, pp. 183-190; Reddy, K.K., Rajadurai, S., Nayudamma, Y., Studies on Dhava (Anogiessus latifolia) tannins: Part III-polyphenols of bark, sapwood and heartwood of Dhava (1965) Indian J. Chem., 3, pp. 308-310; Roy, G.P., Chaturvedi, D.P., Ethnomedicinal trees of Abujh-Marh area, Madhya Pradesh (1986) Folklore, 27, pp. 95-100; Scalbert, A., Monties, B., Favre, J.M., Polyphenols of Quercus robur. adult tree and in votro grown calli and shoots (1988) Phytochemistry, 27, pp. 3483-3488; Schmeda, H.G., Gutierrez, M.I., Loyola, J.I., Zuniga, J., Biological activity and xanthine oxidase inhibitors from Scirpus califormicus (C.A. Mey.) Steud (1996) Phytother. Res., 10, pp. 683-685; Sreejayan, N., Rao, M.N.A., Free radical scavenging activity of curcumnoids (1996) Drug Res., 46, pp. 169-171; Taylor, W.H., Sinha, A., Khan, I.A., McDaniel, S.T., Esko, J.D., Primers of glycosaminoglycan biosynthesis from Peruvian rain forest plants (1998) J. Biol. Chem., 273, pp. 22260-22266; Theismania, K.C., Kuttan, R., Inhibition of liver fibrosis by ellagic acid (1996) Indian J. Physiol. Pharmacol., 40, pp. 363-366

PY - 2005

Y1 - 2005

N2 - Oxidative stress is an important causative factor in several human chronic diseases, such as atherosclerosis, cardiovascular disorders, mutagenesis, cancer, several neurodegenerative disorders, and the aging process. Phenolics and tannins are reported to be good antioxidants. Anogeissus latifolia (Combretaceae) bark has been used in the Indian traditional systems of medicine for curing a variety of ailments, but scientific validation is not available till date. Hence the present study was undertaken to isolate antioxidant compounds by activity-guided isolation. Inhibtion of diphenyl picryl hydrazyl (DPPH) and Xanthine oxidase along with photochemiluminescence assay were used as bioassay for antioxidant activity. Activity guided isolation was carried out using silica column and the compounds were quantified using HPLC. Ethyl acetate and butanol fraction exhibited potent antioxidant activity. Bioassay-guided isolation led to isolation of ellagic acid (1) and dimethyl ellagic acid (2) as the main active compounds, which along with gallic acid were quantified by HPLC. Thus we conclude that these three major tannoid principles present in A. latifolia, are responsible for the antioxidant potential and possibly their therapeutic potential.

AB - Oxidative stress is an important causative factor in several human chronic diseases, such as atherosclerosis, cardiovascular disorders, mutagenesis, cancer, several neurodegenerative disorders, and the aging process. Phenolics and tannins are reported to be good antioxidants. Anogeissus latifolia (Combretaceae) bark has been used in the Indian traditional systems of medicine for curing a variety of ailments, but scientific validation is not available till date. Hence the present study was undertaken to isolate antioxidant compounds by activity-guided isolation. Inhibtion of diphenyl picryl hydrazyl (DPPH) and Xanthine oxidase along with photochemiluminescence assay were used as bioassay for antioxidant activity. Activity guided isolation was carried out using silica column and the compounds were quantified using HPLC. Ethyl acetate and butanol fraction exhibited potent antioxidant activity. Bioassay-guided isolation led to isolation of ellagic acid (1) and dimethyl ellagic acid (2) as the main active compounds, which along with gallic acid were quantified by HPLC. Thus we conclude that these three major tannoid principles present in A. latifolia, are responsible for the antioxidant potential and possibly their therapeutic potential.

M3 - Article

VL - 11

SP - 174

EP - 178

JO - Natural Product Sciences

JF - Natural Product Sciences

SN - 1226-3907

IS - 3

ER -

Govindarajan R, Vijayakumar M, Shirwaikar A, Rawat AKS, Mehrotra S, Pushpangadan P. Activity guided isolation of antioxidant tannoid principles from Anogeissus latifolia. Natural Product Sciences. 2005;11(3):174-178.