Adaptive response to low dose of EMS or MMS in human peripheral blood lymphocytes

S. K. Harish, K. P. Guruprasad, Riaz Mahmood, V. Vasudev, K. R. Manjunath, G. K. Chethan

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Human peripheral blood lymphocytes stimulated in vitro for 6 hr were exposed to a low (conditioning) dose of ethyl methanesulfonate (EMS; 1.5×10-4 M) or methyl methanesulfonate (MMS; 1.5×10-5 M). After 6 hr, the cells were treated with a high (challenging) concentration of the same agent (1.5×10-3 M EMS or 1.5×10-4 M MMS). The cells that received both conditioning and challenging doses became less sensitive to the induction of sister chromatid exchanges (SCEs) than those which did not receive the pretreatment with EMS or MMS. They responded with lower frequencies of SCEs. This suggests that conditioning dose of EMS or MMS has offered the lymphocytes to have decreased SCEs. This led to the realization that pre-exposure of lymphocytes to low dose can cause the induction of repair activity. This is a clear indication of the existence of adaptive response induced by alkylating agents whether it is ethylating or methylating in human lymphocytes in vitro.

    Original languageEnglish
    Pages (from-to)1147-1150
    Number of pages4
    JournalIndian Journal of Experimental Biology
    Volume36
    Issue number11
    Publication statusPublished - 11-1998

    Fingerprint

    Sister Chromatid Exchange
    Lymphocytes
    Ethyl Methanesulfonate
    Methyl Methanesulfonate
    Alkylating Agents
    In Vitro Techniques

    All Science Journal Classification (ASJC) codes

    • Biotechnology
    • Medicine(all)
    • Molecular Biology
    • Cell Biology

    Cite this

    Harish, S. K., Guruprasad, K. P., Mahmood, R., Vasudev, V., Manjunath, K. R., & Chethan, G. K. (1998). Adaptive response to low dose of EMS or MMS in human peripheral blood lymphocytes. Indian Journal of Experimental Biology, 36(11), 1147-1150.
    Harish, S. K. ; Guruprasad, K. P. ; Mahmood, Riaz ; Vasudev, V. ; Manjunath, K. R. ; Chethan, G. K. / Adaptive response to low dose of EMS or MMS in human peripheral blood lymphocytes. In: Indian Journal of Experimental Biology. 1998 ; Vol. 36, No. 11. pp. 1147-1150.
    @article{62ce475ff28f4103adf53a34721ab339,
    title = "Adaptive response to low dose of EMS or MMS in human peripheral blood lymphocytes",
    abstract = "Human peripheral blood lymphocytes stimulated in vitro for 6 hr were exposed to a low (conditioning) dose of ethyl methanesulfonate (EMS; 1.5×10-4 M) or methyl methanesulfonate (MMS; 1.5×10-5 M). After 6 hr, the cells were treated with a high (challenging) concentration of the same agent (1.5×10-3 M EMS or 1.5×10-4 M MMS). The cells that received both conditioning and challenging doses became less sensitive to the induction of sister chromatid exchanges (SCEs) than those which did not receive the pretreatment with EMS or MMS. They responded with lower frequencies of SCEs. This suggests that conditioning dose of EMS or MMS has offered the lymphocytes to have decreased SCEs. This led to the realization that pre-exposure of lymphocytes to low dose can cause the induction of repair activity. This is a clear indication of the existence of adaptive response induced by alkylating agents whether it is ethylating or methylating in human lymphocytes in vitro.",
    author = "Harish, {S. K.} and Guruprasad, {K. P.} and Riaz Mahmood and V. Vasudev and Manjunath, {K. R.} and Chethan, {G. K.}",
    year = "1998",
    month = "11",
    language = "English",
    volume = "36",
    pages = "1147--1150",
    journal = "Journal of scientific & industrial research. C. Biological sciences",
    issn = "0019-5189",
    publisher = "National Institute of Science Communication",
    number = "11",

    }

    Harish, SK, Guruprasad, KP, Mahmood, R, Vasudev, V, Manjunath, KR & Chethan, GK 1998, 'Adaptive response to low dose of EMS or MMS in human peripheral blood lymphocytes', Indian Journal of Experimental Biology, vol. 36, no. 11, pp. 1147-1150.

    Adaptive response to low dose of EMS or MMS in human peripheral blood lymphocytes. / Harish, S. K.; Guruprasad, K. P.; Mahmood, Riaz; Vasudev, V.; Manjunath, K. R.; Chethan, G. K.

    In: Indian Journal of Experimental Biology, Vol. 36, No. 11, 11.1998, p. 1147-1150.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Adaptive response to low dose of EMS or MMS in human peripheral blood lymphocytes

    AU - Harish, S. K.

    AU - Guruprasad, K. P.

    AU - Mahmood, Riaz

    AU - Vasudev, V.

    AU - Manjunath, K. R.

    AU - Chethan, G. K.

    PY - 1998/11

    Y1 - 1998/11

    N2 - Human peripheral blood lymphocytes stimulated in vitro for 6 hr were exposed to a low (conditioning) dose of ethyl methanesulfonate (EMS; 1.5×10-4 M) or methyl methanesulfonate (MMS; 1.5×10-5 M). After 6 hr, the cells were treated with a high (challenging) concentration of the same agent (1.5×10-3 M EMS or 1.5×10-4 M MMS). The cells that received both conditioning and challenging doses became less sensitive to the induction of sister chromatid exchanges (SCEs) than those which did not receive the pretreatment with EMS or MMS. They responded with lower frequencies of SCEs. This suggests that conditioning dose of EMS or MMS has offered the lymphocytes to have decreased SCEs. This led to the realization that pre-exposure of lymphocytes to low dose can cause the induction of repair activity. This is a clear indication of the existence of adaptive response induced by alkylating agents whether it is ethylating or methylating in human lymphocytes in vitro.

    AB - Human peripheral blood lymphocytes stimulated in vitro for 6 hr were exposed to a low (conditioning) dose of ethyl methanesulfonate (EMS; 1.5×10-4 M) or methyl methanesulfonate (MMS; 1.5×10-5 M). After 6 hr, the cells were treated with a high (challenging) concentration of the same agent (1.5×10-3 M EMS or 1.5×10-4 M MMS). The cells that received both conditioning and challenging doses became less sensitive to the induction of sister chromatid exchanges (SCEs) than those which did not receive the pretreatment with EMS or MMS. They responded with lower frequencies of SCEs. This suggests that conditioning dose of EMS or MMS has offered the lymphocytes to have decreased SCEs. This led to the realization that pre-exposure of lymphocytes to low dose can cause the induction of repair activity. This is a clear indication of the existence of adaptive response induced by alkylating agents whether it is ethylating or methylating in human lymphocytes in vitro.

    UR - http://www.scopus.com/inward/record.url?scp=0032200239&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0032200239&partnerID=8YFLogxK

    M3 - Article

    VL - 36

    SP - 1147

    EP - 1150

    JO - Journal of scientific & industrial research. C. Biological sciences

    JF - Journal of scientific & industrial research. C. Biological sciences

    SN - 0019-5189

    IS - 11

    ER -