Advances in adaptive optics–based two-photon fluorescence microscopy for brain imaging

Pranoy Sahu, Nirmal Mazumder

Research output: Contribution to journalReview article

Abstract

Deep tissue imaging using two-photon fluorescence (TPF) techniques have revolutionized the optical imaging community by providing in depth molecular information at the single-cell level. These techniques provide structural and functional aspects of mammalian brain at unprecedented depth and resolution. However, wavefront distortions introduced by the optical system as well as the biological sample (tissue) limit the achievable fluorescence signal-to-noise ratio and resolution with penetration depth. In this review, we discuss on the advances in TPF microscopy techniques for in vivo functional imaging and offer guidelines as to which technologies are best suited for different imaging applications with special reference to adaptive optics.

Original languageEnglish
JournalLasers in Medical Science
DOIs
Publication statusAccepted/In press - 01-01-2019

Fingerprint

Photons
Fluorescence Microscopy
Neuroimaging
Fluorescence
Optical Devices
Optical Imaging
Signal-To-Noise Ratio
Guidelines
Technology
Brain

All Science Journal Classification (ASJC) codes

  • Surgery
  • Dermatology

Cite this

@article{4a8c9f51a0764f92a53cd44d9694484d,
title = "Advances in adaptive optics–based two-photon fluorescence microscopy for brain imaging",
abstract = "Deep tissue imaging using two-photon fluorescence (TPF) techniques have revolutionized the optical imaging community by providing in depth molecular information at the single-cell level. These techniques provide structural and functional aspects of mammalian brain at unprecedented depth and resolution. However, wavefront distortions introduced by the optical system as well as the biological sample (tissue) limit the achievable fluorescence signal-to-noise ratio and resolution with penetration depth. In this review, we discuss on the advances in TPF microscopy techniques for in vivo functional imaging and offer guidelines as to which technologies are best suited for different imaging applications with special reference to adaptive optics.",
author = "Pranoy Sahu and Nirmal Mazumder",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/s10103-019-02908-z",
language = "English",
journal = "Lasers in Medical Science",
issn = "0268-8921",
publisher = "Springer London",

}

Advances in adaptive optics–based two-photon fluorescence microscopy for brain imaging. / Sahu, Pranoy; Mazumder, Nirmal.

In: Lasers in Medical Science, 01.01.2019.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Advances in adaptive optics–based two-photon fluorescence microscopy for brain imaging

AU - Sahu, Pranoy

AU - Mazumder, Nirmal

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Deep tissue imaging using two-photon fluorescence (TPF) techniques have revolutionized the optical imaging community by providing in depth molecular information at the single-cell level. These techniques provide structural and functional aspects of mammalian brain at unprecedented depth and resolution. However, wavefront distortions introduced by the optical system as well as the biological sample (tissue) limit the achievable fluorescence signal-to-noise ratio and resolution with penetration depth. In this review, we discuss on the advances in TPF microscopy techniques for in vivo functional imaging and offer guidelines as to which technologies are best suited for different imaging applications with special reference to adaptive optics.

AB - Deep tissue imaging using two-photon fluorescence (TPF) techniques have revolutionized the optical imaging community by providing in depth molecular information at the single-cell level. These techniques provide structural and functional aspects of mammalian brain at unprecedented depth and resolution. However, wavefront distortions introduced by the optical system as well as the biological sample (tissue) limit the achievable fluorescence signal-to-noise ratio and resolution with penetration depth. In this review, we discuss on the advances in TPF microscopy techniques for in vivo functional imaging and offer guidelines as to which technologies are best suited for different imaging applications with special reference to adaptive optics.

UR - http://www.scopus.com/inward/record.url?scp=85075245477&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85075245477&partnerID=8YFLogxK

U2 - 10.1007/s10103-019-02908-z

DO - 10.1007/s10103-019-02908-z

M3 - Review article

C2 - 31729608

AN - SCOPUS:85075245477

JO - Lasers in Medical Science

JF - Lasers in Medical Science

SN - 0268-8921

ER -