Alcoholic stem extract of Coscinium fenestratum regulates carbohydrate metabolism and improves antioxidant status in streptozotocin-nicotinamide induced diabetic rats

I.S.R. Punitha, K. Rajendran, A. Shirwaikar

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99 Citations (Scopus)

Abstract

Alcoholic extract of the stems of Coscinium fenestratum, a medicinal plant indigenous to India and Sri Lanka used in ayurveda and siddha medicine for treating diabetes, was studied for its carbohydrate metabolism effect and antioxidant status in streptozotocin-nicotinamide induced type 2 diabetic rats. Oral administration of C. fenestratum stem extract in graded doses caused a significant increase in enzymatic antioxidants such as catalase, superoxide dismutase, glutathione synthetase, peroxidase, and glutathione peroxidase and in the nonenzymatic antioxidants ascorbic acid, ceruloplasmin and tocopherol. Effects of alcoholic extract on glycolytic enzymes such as glucose-6-phosphate dehydrogenase, lactate dehydrogenase and hexokinase showed a significant increase in their levels, whereas a significant decrease was observed in the levels of gluconeogenic enzyme, glucose-6-phosphatase and alanine aminotransferase in treated diabetic rats. Serum creatinine and urea levels also declined significantly. This investigation demonstrates significant antidiabetic activity of C. fenestratum. © The Author (2005). Published by Oxford University Press. All rights reserved.
Original languageEnglish
Pages (from-to)375-381
Number of pages7
JournalEvidence-based Complementary and Alternative Medicine
Volume2
Issue number3
DOIs
Publication statusPublished - 2005

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Niacinamide
Carbohydrate Metabolism
Streptozocin
Antioxidants
Glutathione Peroxidase
Glutathione Synthase
Ayurvedic Medicine
Glucose-6-Phosphatase
Sri Lanka
Ceruloplasmin
Hexokinase
Tocopherols
Glucosephosphate Dehydrogenase
Enzymes
Medicinal Plants
Alanine Transaminase
L-Lactate Dehydrogenase
Hypoglycemic Agents
Catalase
Ascorbic Acid

Cite this

@article{ab3d267f8dfc4b4f95a509e99a80f9a2,
title = "Alcoholic stem extract of Coscinium fenestratum regulates carbohydrate metabolism and improves antioxidant status in streptozotocin-nicotinamide induced diabetic rats",
abstract = "Alcoholic extract of the stems of Coscinium fenestratum, a medicinal plant indigenous to India and Sri Lanka used in ayurveda and siddha medicine for treating diabetes, was studied for its carbohydrate metabolism effect and antioxidant status in streptozotocin-nicotinamide induced type 2 diabetic rats. Oral administration of C. fenestratum stem extract in graded doses caused a significant increase in enzymatic antioxidants such as catalase, superoxide dismutase, glutathione synthetase, peroxidase, and glutathione peroxidase and in the nonenzymatic antioxidants ascorbic acid, ceruloplasmin and tocopherol. Effects of alcoholic extract on glycolytic enzymes such as glucose-6-phosphate dehydrogenase, lactate dehydrogenase and hexokinase showed a significant increase in their levels, whereas a significant decrease was observed in the levels of gluconeogenic enzyme, glucose-6-phosphatase and alanine aminotransferase in treated diabetic rats. Serum creatinine and urea levels also declined significantly. This investigation demonstrates significant antidiabetic activity of C. fenestratum. {\circledC} The Author (2005). Published by Oxford University Press. All rights reserved.",
author = "I.S.R. Punitha and K. Rajendran and A. Shirwaikar",
note = "Cited By :85 Export Date: 10 November 2017 Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal, Karnataka 576104, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: alanine aminotransferase, 9000-86-6, 9014-30-6; alcohol, 64-17-5; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; catalase, 9001-05-2; ceruloplasmin, 9031-37-2; creatinine, 19230-81-0, 60-27-5; glucose 6 phosphate dehydrogenase, 37259-83-9, 9001-40-5; glutathione peroxidase, 9013-66-5; glutathione synthase, 9023-62-5; hexokinase, 9001-51-8; lactate dehydrogenase, 9001-60-9; nicotinamide, 11032-50-1, 98-92-0; peroxidase, 9003-99-0; streptozocin, 18883-66-4; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; tocopherol, 1406-66-2; urea, 57-13-6 Manufacturers: Sigma Aldrich, Germany References: Mohamed, A.K., Bierhaus, A., Schiekofer, S., Tritschlet, H., Ziegler, H., Nawroth, P.P., The role of oxidative stress and NF (B) activation in late diabetic complications (1999) Biofactors, 10, pp. 175-179; Oberley, L.W., Free radicals and diabetes (1988) Free Radic Biol Med, 5, pp. 113-124; Halliwell, B., Gutteridge, J.M.C., Lipid peroxidation, oxygen radicals, cell damage and antioxidant therapy (1994) Lancet, 344, pp. 1396-1397; Simmons, K.J., Defense against free radicals has therapeutic implications (1984) J Am Med Assoc, 251, pp. 2187-2192; Wohaieb, S.A., Godin, D.V., Alterations in free radical tissue defense mechanism in STZ induced diabetes in rat, effects of insulin treatment (1987) Diabetes, 36, pp. 1014-1018; Varier, P.S., Coscinium fenestratum (1994) Indian Medicinal Plants, Compendium of 500 Species, 2, pp. 191-193. , Hyderabad, India: Orient Longman Ltd; Coscinium fenestratum (1950) The Wealth of India, Publication Information and Directorate, 2, p. 360. , India: CSIR; Datta, S.C., Mathur, R.K., Baruah, J.N., Minor alkaloids of Coscinium fenestratum root (1988) Indian Drugs, 25, p. 350; Singh, G.B., Singh, S., Bani, S., Malhotra, S., Hypotensive action of a Coscinium fenestratum stem extract (1990) J Ethnopharmacol, 38, pp. 151-155; Venukumar, M.R., Latha, M.S., Effect of Coscinium fenestratum on hepatotoxicity in rats (2004) Indian J Exp Biol, 42, pp. 792-797; Shirwaikar, A., Rajendran, K., Punitha, I.S.R., Antidiabetic activity of alcoholic stem extract of C. fenestratum in streptozotocin nicotinamide induced type 2 diabetic rats (2005) J Ethnopharmacol, 97, pp. 369-374; Kokate, C.K., Purohit, A.P., Gokhale, S.B., Phytochemical Screening (1994) Practical Pharmacognosy, pp. 107-113. , New Delhi: Vallabh Prakashan; Harborne, J.B., Methods of Extraction and Isolation (1998) Phytochemical Methods, pp. 60-66. , London: Chapman & Hall; Masiello, P., Broca, C., Gross, R., Roye, M., Manteghetti, M., Hillaire-Buys, D., Development of a new model of type 2 diabetes in adult rats administered with streptozotocin and nicotinamide (1998) Diabetes, 47, p. 224; Shirwaikar, A., Rajendran, K., Dinesh Kumar, C., Oral antidiabetic activity of Annona squamosa leaf alcohol extract in NIDDM rats (2004) Pharmaceut Biol, 42, pp. 30-35; Shirwaikar, A., Rajendran, K., Dinesh Kumar, C., Bodla, R.G., Antidiabetic activity of aqueous leaf extract of Annona squamosa in streptozotocin nicotinamide type 2 diabetic rats (2004) J Ethnopharmacol, 91, pp. 171-175; Brandstrup, N., Kirk, J.E., Bruni, C., Determination of hexokinase in tissues (1957) J Gerontol, 12, pp. 166-171; Deutsch, J., Glucose 6 Phosphate dehydrogenase (1983) Bergmeyer Methods of Enzymatic Analysis, 3, p. 190. , 3rd edition. Deerfield Beach, FL: Verlag Chemie; Vassault, A., Lactate dehydrogenase (1983) Bergmeyer Methods of Enzymatic Analysis, 3, p. 118. , 3rd edition. Deerfield Beach, FL: Verlag Chemie; Baginsky, E.S., Foa, P.P., Zad, B., Glucose 6-phosphatase (1974) Bergmeyer's Methods in Enzymatic Analysis, 2, pp. 788-792. , New York: Academic Press; Horder, M., Rej, R., Alanine amino transferase (1983) Bergmeyer Methods of Enzymatic Analysis, 3, p. 190. , 3rd edition. Deerfield Beach, FL: Verlag Chemie; Rotruck, J.T., Pope, A.L., Ganther, H.E., Swanson, A.B., Hafeman, D.G., Hoekstra, W.G., Selenium biochemical role as a component of glutathione peroxide purification and assay (1973) Science, 179, pp. 588-590; Aebi, E.H., Catalase (1983) Bergmeyer Methods of Enzymatic Analysis, 3, p. 273. , 3rd edition. Deerfield Beach, FL: Verlag Chemie; Sinha, K.A., Colorimetric assay of catalase (1972) Anal Biochem, 47, pp. 389-394; Putter, J., Becker, R., Peroxidase (1983) Bergmeyer Methods of Enzymatic Analysis, 3, p. 286. , 3rd Edition. Deerfield Beach, Florida: Verlag Chemie; Kakkar, P., Dos, B., Viswanathan, P.N., A modified spectrophotometric assay of superoxide dismutase (1984) Ind J Biochem Biophys, 21, pp. 130-132; Ravin, H.A., An improved colorimetric assay of ceruloplasmin (1961) J Labor Clin Med, 589, pp. 161-168; Desai, L.D., Vitamin E analysis methods for animal tissues (1984) Methods Enzymol, 105, pp. 138-142; Omaye, S.T., Turnball, J.D., Sauberlich, H.E., Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids (1979) Methods in Enzymology, 62, pp. 1-11. , McCormick DB, Wright CD (eds). New York: Academic Press; Lowry, O.H., Roesborough, M.J., Farr, A.L., Randall, R.J., Protein measurement with folin- phenol reagent (1951) J Biol Chem, 193, pp. 265-275; Baquer, N.Z., Gupta, D., Raju, J., Regulation of metabolic pathways in liver and kidney during experimetal diabetes: Effects of antidiabetic compounds (1998) Ind J Clin Biochem, 13, pp. 63-80; Ugochukwu, N.H., Babady, N.E., Antihyperglycaemic effect of aqueous and ethanolic extract of Gongronema latifolium levels of glucose and glycogen metabolism in liver of normal and STZ induced diabetic rats (2003) Life Science, 73, pp. 1924-1938; Burtis, C.A., Ashwood, E.R., Carbohydrate metabolism. Methods of Extraction and Isolation (1998) Tietz Textbook of Clinical Chemistry, pp. 594-655. , W.B. Saunder's Company, Medina, USA. 3rd edition; Berg, J.M., Tymoczko, J.L., Stryer, L., Glycolysis and gluconeogenesis (2001) Biochemistry, pp. 425-464. , Berg JM, Tymoczko JL, Stryer L (eds). New York: W.H. Freeman and Company; Ghosh, S., Suryawanshi, S.A., Effect of Vinca rosea extracts in treatment of alloxan diabetes in male albino rats (2001) Ind J Expt Biol, 39, pp. 748-759; Felig, P., Marliss, E., Ohman, J., Cahill, J.F., Plasma aminoacid levels in diabetic ketoacidosis (1970) Diabetes, 19, pp. 727-730; Halliwell, B., Gutteridge, J.M.C., Free Radicals and Toxicology (1985) Free Radicals in Biology and Medicine, pp. 1-27. , Oxford: Clarendon Press; Mccord, J.M., Keele, B.B., Fridovich, I., An enzyme based theory of obligate anaerobiosis, the physiological functions of superoxide dismutase (1976) Proc Natl Acad Sci USA, 68, pp. 1024-1027; Chance, B., Greenstein, D.S., Roughton, R.J.W., The mechanism of catalase action - Steady state analysis (1952) Arch Biochem Biophys, 37, pp. 301-339; Searle, A.J., Wilson, R., Glutathione perioxide effect of superoxide, hydroxyl and bromine free radicals on enzyme activity (1980) Int J Radiat Biol, 37, pp. 213-217; Meister, A., New aspects of glutathione biochemistry and transport selective alterations of glutathione metabolism (1984) Nutr Rev, 42, pp. 397-410; Nicotera, P., Orrenius, S., Role of thiols in protection against biological reactive intermediates (1986) Adv Exp Med Biol, 197, pp. 41-49; Anuradha, C.V., Selvam, R., Effect of oral methionine on tissue lipid peroxidation and antioxidants in alloxan induced diabetic rats (1993) J Nutr Biochem, 4, pp. 212-217; Bruce, A., Freeman, D., James, C., Biology of disease-free radicals and tissue injury (1982) Lab Invest, 47, pp. 412-426; Kinalski, M., Sledziewski, A., Telejko, B., Zarzycki, W., Kinalska, T., Lipid peroxidation and scavenging enzyme activity in streptozotocin induced diabetes (2000) Acta Diabetologia, 37, pp. 179-183; Halliwell, B., Gutteridge, J.M.C., The antioxidant of human extra cellular fluids (1990) Arch Biochem Biophys, 280, pp. 1-8; Frei, B., England, L., Ames, B.N., Ascorbate is an outstanding antioxidant in human blood plasma (1986) Proc Natl Acad Sci USA, 86, pp. 6377-6381; Inefers, H., Sies, H., The protection by ascorbate and glutathione against microsomal lipid peroxidation is dependent on vitamin E (1988) Eur J Biochem, 174, pp. 353-357; Sajithlal, G.B., Chitra, P., Chandrakasan, G., Effect of curcumin on the advanced glycation and cross linking of collagen in diabetic rats (1998) Biochem and Pharmacol, 56, pp. 1607-1614; Jin, X.L., Shao, Y., Wang, M.J., Chen, L.J., Jin, G.Z., Tetrahydro protoberberines inhibit lipid peroxidation and scavenge hydroxyl free radicals (2000) Acta Pharmacol Sin, 21, pp. 477-480; Auddy, B., Ferreiru, M., Blasina, F., Lafon, L., Arredondo, F., Dajas, F., Screening of antioxidant activity of three Indian medicinal plant traditionally used for the management of neurodegerative diseases (2003) J Ethnopharmacol, 84, pp. 131-138; Hung, C.Y., Yen, G.C., Antioxidant activity of phenolic compounds isolated from Mesona procumbens Hemsl (2002) J Agric Food Chem, 50, pp. 2993-2997",
year = "2005",
doi = "10.1093/ecam/neh099",
language = "English",
volume = "2",
pages = "375--381",
journal = "Evidence-based Complementary and Alternative Medicine",
issn = "1741-427X",
publisher = "Hindawi Publishing Corporation",
number = "3",

}

TY - JOUR

T1 - Alcoholic stem extract of Coscinium fenestratum regulates carbohydrate metabolism and improves antioxidant status in streptozotocin-nicotinamide induced diabetic rats

AU - Punitha, I.S.R.

AU - Rajendran, K.

AU - Shirwaikar, A.

N1 - Cited By :85 Export Date: 10 November 2017 Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal, Karnataka 576104, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: alanine aminotransferase, 9000-86-6, 9014-30-6; alcohol, 64-17-5; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; catalase, 9001-05-2; ceruloplasmin, 9031-37-2; creatinine, 19230-81-0, 60-27-5; glucose 6 phosphate dehydrogenase, 37259-83-9, 9001-40-5; glutathione peroxidase, 9013-66-5; glutathione synthase, 9023-62-5; hexokinase, 9001-51-8; lactate dehydrogenase, 9001-60-9; nicotinamide, 11032-50-1, 98-92-0; peroxidase, 9003-99-0; streptozocin, 18883-66-4; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; tocopherol, 1406-66-2; urea, 57-13-6 Manufacturers: Sigma Aldrich, Germany References: Mohamed, A.K., Bierhaus, A., Schiekofer, S., Tritschlet, H., Ziegler, H., Nawroth, P.P., The role of oxidative stress and NF (B) activation in late diabetic complications (1999) Biofactors, 10, pp. 175-179; Oberley, L.W., Free radicals and diabetes (1988) Free Radic Biol Med, 5, pp. 113-124; Halliwell, B., Gutteridge, J.M.C., Lipid peroxidation, oxygen radicals, cell damage and antioxidant therapy (1994) Lancet, 344, pp. 1396-1397; Simmons, K.J., Defense against free radicals has therapeutic implications (1984) J Am Med Assoc, 251, pp. 2187-2192; Wohaieb, S.A., Godin, D.V., Alterations in free radical tissue defense mechanism in STZ induced diabetes in rat, effects of insulin treatment (1987) Diabetes, 36, pp. 1014-1018; Varier, P.S., Coscinium fenestratum (1994) Indian Medicinal Plants, Compendium of 500 Species, 2, pp. 191-193. , Hyderabad, India: Orient Longman Ltd; Coscinium fenestratum (1950) The Wealth of India, Publication Information and Directorate, 2, p. 360. , India: CSIR; Datta, S.C., Mathur, R.K., Baruah, J.N., Minor alkaloids of Coscinium fenestratum root (1988) Indian Drugs, 25, p. 350; Singh, G.B., Singh, S., Bani, S., Malhotra, S., Hypotensive action of a Coscinium fenestratum stem extract (1990) J Ethnopharmacol, 38, pp. 151-155; Venukumar, M.R., Latha, M.S., Effect of Coscinium fenestratum on hepatotoxicity in rats (2004) Indian J Exp Biol, 42, pp. 792-797; Shirwaikar, A., Rajendran, K., Punitha, I.S.R., Antidiabetic activity of alcoholic stem extract of C. fenestratum in streptozotocin nicotinamide induced type 2 diabetic rats (2005) J Ethnopharmacol, 97, pp. 369-374; Kokate, C.K., Purohit, A.P., Gokhale, S.B., Phytochemical Screening (1994) Practical Pharmacognosy, pp. 107-113. , New Delhi: Vallabh Prakashan; Harborne, J.B., Methods of Extraction and Isolation (1998) Phytochemical Methods, pp. 60-66. , London: Chapman & Hall; Masiello, P., Broca, C., Gross, R., Roye, M., Manteghetti, M., Hillaire-Buys, D., Development of a new model of type 2 diabetes in adult rats administered with streptozotocin and nicotinamide (1998) Diabetes, 47, p. 224; Shirwaikar, A., Rajendran, K., Dinesh Kumar, C., Oral antidiabetic activity of Annona squamosa leaf alcohol extract in NIDDM rats (2004) Pharmaceut Biol, 42, pp. 30-35; Shirwaikar, A., Rajendran, K., Dinesh Kumar, C., Bodla, R.G., Antidiabetic activity of aqueous leaf extract of Annona squamosa in streptozotocin nicotinamide type 2 diabetic rats (2004) J Ethnopharmacol, 91, pp. 171-175; Brandstrup, N., Kirk, J.E., Bruni, C., Determination of hexokinase in tissues (1957) J Gerontol, 12, pp. 166-171; Deutsch, J., Glucose 6 Phosphate dehydrogenase (1983) Bergmeyer Methods of Enzymatic Analysis, 3, p. 190. , 3rd edition. Deerfield Beach, FL: Verlag Chemie; Vassault, A., Lactate dehydrogenase (1983) Bergmeyer Methods of Enzymatic Analysis, 3, p. 118. , 3rd edition. Deerfield Beach, FL: Verlag Chemie; Baginsky, E.S., Foa, P.P., Zad, B., Glucose 6-phosphatase (1974) Bergmeyer's Methods in Enzymatic Analysis, 2, pp. 788-792. , New York: Academic Press; Horder, M., Rej, R., Alanine amino transferase (1983) Bergmeyer Methods of Enzymatic Analysis, 3, p. 190. , 3rd edition. Deerfield Beach, FL: Verlag Chemie; Rotruck, J.T., Pope, A.L., Ganther, H.E., Swanson, A.B., Hafeman, D.G., Hoekstra, W.G., Selenium biochemical role as a component of glutathione peroxide purification and assay (1973) Science, 179, pp. 588-590; Aebi, E.H., Catalase (1983) Bergmeyer Methods of Enzymatic Analysis, 3, p. 273. , 3rd edition. Deerfield Beach, FL: Verlag Chemie; Sinha, K.A., Colorimetric assay of catalase (1972) Anal Biochem, 47, pp. 389-394; Putter, J., Becker, R., Peroxidase (1983) Bergmeyer Methods of Enzymatic Analysis, 3, p. 286. , 3rd Edition. Deerfield Beach, Florida: Verlag Chemie; Kakkar, P., Dos, B., Viswanathan, P.N., A modified spectrophotometric assay of superoxide dismutase (1984) Ind J Biochem Biophys, 21, pp. 130-132; Ravin, H.A., An improved colorimetric assay of ceruloplasmin (1961) J Labor Clin Med, 589, pp. 161-168; Desai, L.D., Vitamin E analysis methods for animal tissues (1984) Methods Enzymol, 105, pp. 138-142; Omaye, S.T., Turnball, J.D., Sauberlich, H.E., Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids (1979) Methods in Enzymology, 62, pp. 1-11. , McCormick DB, Wright CD (eds). New York: Academic Press; Lowry, O.H., Roesborough, M.J., Farr, A.L., Randall, R.J., Protein measurement with folin- phenol reagent (1951) J Biol Chem, 193, pp. 265-275; Baquer, N.Z., Gupta, D., Raju, J., Regulation of metabolic pathways in liver and kidney during experimetal diabetes: Effects of antidiabetic compounds (1998) Ind J Clin Biochem, 13, pp. 63-80; Ugochukwu, N.H., Babady, N.E., Antihyperglycaemic effect of aqueous and ethanolic extract of Gongronema latifolium levels of glucose and glycogen metabolism in liver of normal and STZ induced diabetic rats (2003) Life Science, 73, pp. 1924-1938; Burtis, C.A., Ashwood, E.R., Carbohydrate metabolism. Methods of Extraction and Isolation (1998) Tietz Textbook of Clinical Chemistry, pp. 594-655. , W.B. Saunder's Company, Medina, USA. 3rd edition; Berg, J.M., Tymoczko, J.L., Stryer, L., Glycolysis and gluconeogenesis (2001) Biochemistry, pp. 425-464. , Berg JM, Tymoczko JL, Stryer L (eds). New York: W.H. Freeman and Company; Ghosh, S., Suryawanshi, S.A., Effect of Vinca rosea extracts in treatment of alloxan diabetes in male albino rats (2001) Ind J Expt Biol, 39, pp. 748-759; Felig, P., Marliss, E., Ohman, J., Cahill, J.F., Plasma aminoacid levels in diabetic ketoacidosis (1970) Diabetes, 19, pp. 727-730; Halliwell, B., Gutteridge, J.M.C., Free Radicals and Toxicology (1985) Free Radicals in Biology and Medicine, pp. 1-27. , Oxford: Clarendon Press; Mccord, J.M., Keele, B.B., Fridovich, I., An enzyme based theory of obligate anaerobiosis, the physiological functions of superoxide dismutase (1976) Proc Natl Acad Sci USA, 68, pp. 1024-1027; Chance, B., Greenstein, D.S., Roughton, R.J.W., The mechanism of catalase action - Steady state analysis (1952) Arch Biochem Biophys, 37, pp. 301-339; Searle, A.J., Wilson, R., Glutathione perioxide effect of superoxide, hydroxyl and bromine free radicals on enzyme activity (1980) Int J Radiat Biol, 37, pp. 213-217; Meister, A., New aspects of glutathione biochemistry and transport selective alterations of glutathione metabolism (1984) Nutr Rev, 42, pp. 397-410; Nicotera, P., Orrenius, S., Role of thiols in protection against biological reactive intermediates (1986) Adv Exp Med Biol, 197, pp. 41-49; Anuradha, C.V., Selvam, R., Effect of oral methionine on tissue lipid peroxidation and antioxidants in alloxan induced diabetic rats (1993) J Nutr Biochem, 4, pp. 212-217; Bruce, A., Freeman, D., James, C., Biology of disease-free radicals and tissue injury (1982) Lab Invest, 47, pp. 412-426; Kinalski, M., Sledziewski, A., Telejko, B., Zarzycki, W., Kinalska, T., Lipid peroxidation and scavenging enzyme activity in streptozotocin induced diabetes (2000) Acta Diabetologia, 37, pp. 179-183; Halliwell, B., Gutteridge, J.M.C., The antioxidant of human extra cellular fluids (1990) Arch Biochem Biophys, 280, pp. 1-8; Frei, B., England, L., Ames, B.N., Ascorbate is an outstanding antioxidant in human blood plasma (1986) Proc Natl Acad Sci USA, 86, pp. 6377-6381; Inefers, H., Sies, H., The protection by ascorbate and glutathione against microsomal lipid peroxidation is dependent on vitamin E (1988) Eur J Biochem, 174, pp. 353-357; Sajithlal, G.B., Chitra, P., Chandrakasan, G., Effect of curcumin on the advanced glycation and cross linking of collagen in diabetic rats (1998) Biochem and Pharmacol, 56, pp. 1607-1614; Jin, X.L., Shao, Y., Wang, M.J., Chen, L.J., Jin, G.Z., Tetrahydro protoberberines inhibit lipid peroxidation and scavenge hydroxyl free radicals (2000) Acta Pharmacol Sin, 21, pp. 477-480; Auddy, B., Ferreiru, M., Blasina, F., Lafon, L., Arredondo, F., Dajas, F., Screening of antioxidant activity of three Indian medicinal plant traditionally used for the management of neurodegerative diseases (2003) J Ethnopharmacol, 84, pp. 131-138; Hung, C.Y., Yen, G.C., Antioxidant activity of phenolic compounds isolated from Mesona procumbens Hemsl (2002) J Agric Food Chem, 50, pp. 2993-2997

PY - 2005

Y1 - 2005

N2 - Alcoholic extract of the stems of Coscinium fenestratum, a medicinal plant indigenous to India and Sri Lanka used in ayurveda and siddha medicine for treating diabetes, was studied for its carbohydrate metabolism effect and antioxidant status in streptozotocin-nicotinamide induced type 2 diabetic rats. Oral administration of C. fenestratum stem extract in graded doses caused a significant increase in enzymatic antioxidants such as catalase, superoxide dismutase, glutathione synthetase, peroxidase, and glutathione peroxidase and in the nonenzymatic antioxidants ascorbic acid, ceruloplasmin and tocopherol. Effects of alcoholic extract on glycolytic enzymes such as glucose-6-phosphate dehydrogenase, lactate dehydrogenase and hexokinase showed a significant increase in their levels, whereas a significant decrease was observed in the levels of gluconeogenic enzyme, glucose-6-phosphatase and alanine aminotransferase in treated diabetic rats. Serum creatinine and urea levels also declined significantly. This investigation demonstrates significant antidiabetic activity of C. fenestratum. © The Author (2005). Published by Oxford University Press. All rights reserved.

AB - Alcoholic extract of the stems of Coscinium fenestratum, a medicinal plant indigenous to India and Sri Lanka used in ayurveda and siddha medicine for treating diabetes, was studied for its carbohydrate metabolism effect and antioxidant status in streptozotocin-nicotinamide induced type 2 diabetic rats. Oral administration of C. fenestratum stem extract in graded doses caused a significant increase in enzymatic antioxidants such as catalase, superoxide dismutase, glutathione synthetase, peroxidase, and glutathione peroxidase and in the nonenzymatic antioxidants ascorbic acid, ceruloplasmin and tocopherol. Effects of alcoholic extract on glycolytic enzymes such as glucose-6-phosphate dehydrogenase, lactate dehydrogenase and hexokinase showed a significant increase in their levels, whereas a significant decrease was observed in the levels of gluconeogenic enzyme, glucose-6-phosphatase and alanine aminotransferase in treated diabetic rats. Serum creatinine and urea levels also declined significantly. This investigation demonstrates significant antidiabetic activity of C. fenestratum. © The Author (2005). Published by Oxford University Press. All rights reserved.

U2 - 10.1093/ecam/neh099

DO - 10.1093/ecam/neh099

M3 - Article

VL - 2

SP - 375

EP - 381

JO - Evidence-based Complementary and Alternative Medicine

JF - Evidence-based Complementary and Alternative Medicine

SN - 1741-427X

IS - 3

ER -