Alteration in the radiation-induced LD release in HeLa cells by acyclovir

Ganesh Chandra Jagetia, R. Aruna, Bijoor Shivananda Nayak

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aim of the present investigation was to study the effect of various concentrations of acyclovir (ACV) alone or the effect of 0.1 μmol/l acycloir pretreatment on the radion-induced lactate dehydrogenase (LD) release by HeLa cells exposed to 0-3 Gy of γ-radiation. The plateau-phase cells were treated with 0-100 μmol/l ACV for 8 h or 0.1 μmol/l ACV before exposure to 0-3 Gy of γ-radiation. The whole media was removed from the culture and the LD release was measured at 0, 0.5, 1, 2, and 4 h post-drug treatment or post- irradiation. The ACV treatment resulted in a concentration-dependent elevation in the LD release at all the post-treatment time periods. A peak level for LD release was observed at 0 h up to 1 μmol/l ACV and declined thereafter. However, at higher doses a highest elevation in LD release was observed at 1 h post-treatment. Pretreatment of HeLa cells with 0.1 μmol/l ACV before irradiation to different doses of γ-radiation resulted in significant elevation in the release of LD in the medium. The LD release was twofold greater in the ACV + irradiation group at 0 h post-irridiation when compared with the concurrent PBS + γ-radiation group. The highest increase in LD activity was observed at 0 h post-irradiation and a gradual decline thereafter, without restoration to non-drug treated control level up to 4 h post-irradiation. The LD measurements can serve as a short-term assay to evaluate the cytotoxicity of ACV or radiation in vitro. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)129-138
Number of pages10
JournalClinica Chimica Acta
Volume294
Issue number1-2
DOIs
Publication statusPublished - 01-04-2000
Externally publishedYes

Fingerprint

Acyclovir
HeLa Cells
L-Lactate Dehydrogenase
Radiation
Irradiation
Radiation Dosage
Drug therapy
Level control
Cytotoxicity
Restoration
Dosimetry
Assays

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry

Cite this

Jagetia, Ganesh Chandra ; Aruna, R. ; Nayak, Bijoor Shivananda. / Alteration in the radiation-induced LD release in HeLa cells by acyclovir. In: Clinica Chimica Acta. 2000 ; Vol. 294, No. 1-2. pp. 129-138.
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Alteration in the radiation-induced LD release in HeLa cells by acyclovir. / Jagetia, Ganesh Chandra; Aruna, R.; Nayak, Bijoor Shivananda.

In: Clinica Chimica Acta, Vol. 294, No. 1-2, 01.04.2000, p. 129-138.

Research output: Contribution to journalArticle

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