The aim of the present investigation was to study the effect of various concentrations of acyclovir (ACV) alone or the effect of 0.1 μmol/l acycloir pretreatment on the radion-induced lactate dehydrogenase (LD) release by HeLa cells exposed to 0-3 Gy of γ-radiation. The plateau-phase cells were treated with 0-100 μmol/l ACV for 8 h or 0.1 μmol/l ACV before exposure to 0-3 Gy of γ-radiation. The whole media was removed from the culture and the LD release was measured at 0, 0.5, 1, 2, and 4 h post-drug treatment or post- irradiation. The ACV treatment resulted in a concentration-dependent elevation in the LD release at all the post-treatment time periods. A peak level for LD release was observed at 0 h up to 1 μmol/l ACV and declined thereafter. However, at higher doses a highest elevation in LD release was observed at 1 h post-treatment. Pretreatment of HeLa cells with 0.1 μmol/l ACV before irradiation to different doses of γ-radiation resulted in significant elevation in the release of LD in the medium. The LD release was twofold greater in the ACV + irradiation group at 0 h post-irridiation when compared with the concurrent PBS + γ-radiation group. The highest increase in LD activity was observed at 0 h post-irradiation and a gradual decline thereafter, without restoration to non-drug treated control level up to 4 h post-irradiation. The LD measurements can serve as a short-term assay to evaluate the cytotoxicity of ACV or radiation in vitro. (C) 2000 Elsevier Science B.V.
All Science Journal Classification (ASJC) codes
- Clinical Biochemistry