An in vitro chicken gut model for the assessment of phytase producing bacteria

Paul Priyodip, Seetharaman Balaji

Research output: Contribution to journalArticle

Abstract

An in vitro simulated chicken gut model was proposed for studying the phytase activity of selected bacteria such as Streptococcus thermophilus, Sporosarcina pasteurii, Sporosarcina globispora, and Sporosarcina psychrophila using known probiotic bacterium, Lactobacillus helveticus as a control. The selected bacteria were viable in the intestinal lumen and produced extracellular phytase at optimal phytate concentration of 6.25 mM when compared to 3.125 mM and 12.5 mM. These bacteria demonstrated significantly higher (p < 0.05) phosphate liberation (up to 387 µM) due to better phytase activity in the production medium, when compared to the growth medium (339 µM). The phytase activity showed a steady increase in phosphate liberation up to 150 min after which it became constant. This trend is observed for the selected bacteria at pH 5, 6 and 7. However, the liberation of phosphates showed no significant difference (p > 0.05) at the tested pH. Among the analyzed bacteria, the members of the genus Sporosarcina showed better phytate degradation when compared to S. thermophilus. The proposed model can be extended to analyze any extracellular enzymes produced by gut microbes.

Original languageEnglish
Article number294
Journal3 Biotech
Volume9
Issue number8
DOIs
Publication statusPublished - 01-08-2019

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Sporosarcina
6-Phytase
phytases
Chickens
digestive system
chickens
Bacteria
bacterium
Streptococcus thermophilus
bacteria
Phytic Acid
phytic acid
Sporosarcina psychrophila
Sporosarcina globispora
Sporosarcina pasteurii
Lactobacillus helveticus
probiotics
Probiotics
In Vitro Techniques
enzyme

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Science (miscellaneous)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

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abstract = "An in vitro simulated chicken gut model was proposed for studying the phytase activity of selected bacteria such as Streptococcus thermophilus, Sporosarcina pasteurii, Sporosarcina globispora, and Sporosarcina psychrophila using known probiotic bacterium, Lactobacillus helveticus as a control. The selected bacteria were viable in the intestinal lumen and produced extracellular phytase at optimal phytate concentration of 6.25 mM when compared to 3.125 mM and 12.5 mM. These bacteria demonstrated significantly higher (p < 0.05) phosphate liberation (up to 387 µM) due to better phytase activity in the production medium, when compared to the growth medium (339 µM). The phytase activity showed a steady increase in phosphate liberation up to 150 min after which it became constant. This trend is observed for the selected bacteria at pH 5, 6 and 7. However, the liberation of phosphates showed no significant difference (p > 0.05) at the tested pH. Among the analyzed bacteria, the members of the genus Sporosarcina showed better phytate degradation when compared to S. thermophilus. The proposed model can be extended to analyze any extracellular enzymes produced by gut microbes.",
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An in vitro chicken gut model for the assessment of phytase producing bacteria. / Priyodip, Paul; Balaji, Seetharaman.

In: 3 Biotech, Vol. 9, No. 8, 294, 01.08.2019.

Research output: Contribution to journalArticle

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