Analgesic and antiinflammatory activity of leaf extracts of Commiphora caudata in rodents

C.G. Mohan, S. Gokavi, G.L. Viswanatha, H. Shylaja, K. Nandakumar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The present study was undertaken to evaluate the analgesic and anti-inflammatory activity of Alcoholic (70%) and Aqueos extracts of leaves of Commiphora caudata in rodents. The dried powder of the leaves was initially extracted with 70% Alcoholic (ALCC) and Water (AQCC) successively. Acute oral toxicity of ALCC and AQCC were conducted as per OECD guidelines 425. Acute toxicity studies revealed that both the extracts are safe upto 2000mg/kg.The extracts were evaluated for Analgesic activity with formalin induced paw licking test in mice using Pentazocin (2mg/kg,i.p.) as a standard and Anti-inflammatory activity was evaluated with carrageenan induced rat paw edema model in wistar rats using Diclofenac (15mg/kg,i.p) a standard. Acute toxicity results have revealed that both ALCC and AQCC were safe upto 2000 mg/kg dose. In formalin induced paw licking test ALCC at 100, 200 and 400mg/kg, AQCC at 200 and 400mg/kg have showed significant inhibition of paw licking in both early and late phase of the formalin test when compare to control group; In carrageenan induced rat paw edema model ALCC at 200 and 400mg/ kg, AQCC at 400mg/kg have showed significant inhibition in carrageenan induced rat paw when compare to control group, these observations suggests that ALCC and AQCC possess good analgesic and antiinflammatory activity but the Alcoholic is more potent when compare to Aqueous extract.
Original languageEnglish
Pages (from-to)991-998
Number of pages8
JournalPharmacologyonline
Volume2
Publication statusPublished - 2009

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Commiphora
Urodela
Carrageenan
Analgesics
Rodentia
Anti-Inflammatory Agents
Formaldehyde
Edema
Control Groups
Diclofenac
Non-Steroidal Anti-Inflammatory Agents
Pain Measurement
Powders
Wistar Rats
Guidelines
Water

Cite this

Mohan, C.G. ; Gokavi, S. ; Viswanatha, G.L. ; Shylaja, H. ; Nandakumar, K. / Analgesic and antiinflammatory activity of leaf extracts of Commiphora caudata in rodents. In: Pharmacologyonline. 2009 ; Vol. 2. pp. 991-998.
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title = "Analgesic and antiinflammatory activity of leaf extracts of Commiphora caudata in rodents",
abstract = "The present study was undertaken to evaluate the analgesic and anti-inflammatory activity of Alcoholic (70{\%}) and Aqueos extracts of leaves of Commiphora caudata in rodents. The dried powder of the leaves was initially extracted with 70{\%} Alcoholic (ALCC) and Water (AQCC) successively. Acute oral toxicity of ALCC and AQCC were conducted as per OECD guidelines 425. Acute toxicity studies revealed that both the extracts are safe upto 2000mg/kg.The extracts were evaluated for Analgesic activity with formalin induced paw licking test in mice using Pentazocin (2mg/kg,i.p.) as a standard and Anti-inflammatory activity was evaluated with carrageenan induced rat paw edema model in wistar rats using Diclofenac (15mg/kg,i.p) a standard. Acute toxicity results have revealed that both ALCC and AQCC were safe upto 2000 mg/kg dose. In formalin induced paw licking test ALCC at 100, 200 and 400mg/kg, AQCC at 200 and 400mg/kg have showed significant inhibition of paw licking in both early and late phase of the formalin test when compare to control group; In carrageenan induced rat paw edema model ALCC at 200 and 400mg/ kg, AQCC at 400mg/kg have showed significant inhibition in carrageenan induced rat paw when compare to control group, these observations suggests that ALCC and AQCC possess good analgesic and antiinflammatory activity but the Alcoholic is more potent when compare to Aqueous extract.",
author = "C.G. Mohan and S. Gokavi and G.L. Viswanatha and H. Shylaja and K. Nandakumar",
note = "Cited By :2 Export Date: 10 November 2017 Correspondence Address: Viswanatha, G.L.; Preclinical Pharmacology and Toxicology, Connexios Life Sciences Pvt Ltd, J.P. Nagar, Bangalore, India; email: glv_000@yahoo.com Chemicals/CAS: alcohol, 64-17-5; carrageenan, 9000-07-1, 9049-05-2, 9061-82-9, 9064-57-7; diclofenac, 15307-79-6, 15307-86-5; formaldehyde, 50-00-0; pentazocine, 359-83-1, 64024-15-3; water, 7732-18-5 Manufacturers: Cadila, India; Novartis, United States References: Ritter, J.M., Anti-inflammatory and immunosuppressive drugs (2000) Pharmacology, pp. 229-232. , edited by Rang HP, Dale MM, Churchill Livingston, Edinburgh; Haslett C, Chilvers ER, Boon NA, Colledge NR, Hunter JA. Side effects of NSAIDS.In: Davidson's Principles and Practice of Medicine. Churchill Livingstone, Edinburgh 2002:989-990; Shanmugasundaram, P., Venkataraman, S., Antinociceptive activity of Hygrophila auriculata (schum) heine (2005) Afr J Trad Complem Alter Med, 2, pp. 62-69; Prajapathi, Purohit, Sharma, Kumar. In: A hand book of medicinal plants Jodhpur:Dr.Upadesh purohit for Agrobios (India):2003.p.306-7 (sec II); (1996) Indian Materia Medica, , Nadkarni KM, Nadkarni AK, Editors, Bombay; Popular prakashan; Deepa, V.S., Kumar, P.S., Latha, S., Selvamani, P., Srinivasan, S., Antioxidant studies on the ethanolic extract of Commiphora spp (2009) African Journal of Biotechnology, 8 (8), pp. 1630-1636; OECD 2001-gudeline on acute oral toxicity (AOT) Environmental health and safety monograph series on testing and adjustment No.425; Dubuisson, D., Dennis, S.G., The formalin test : A quantitative study of the analgesic effects of morphine, epedrine, and brain stem stimulation in rats and cats (1977) Pain, 4, p. 161; Shibata, M., Ohkubo, T., Takahashi, H., Inoki, R., Modified formalin test characteristic biphasic pain response (1989) Pain, 38, p. 347; Rosland, J.H., Tjoisen, A., Maehle, B., Hole, K., The formalin test in mice:effect of formalin concentration (1990) Pain, 42, p. 235; Vogel, G.H., Vogel, W.H., Scholkens, B.A., Sandow, J., Muller, G., Vogel, W.F., Pharmacological assays (2002) Drug discovery and evaluation, pp. 725-772. , 2nd ed. Springer-Verlag, Berlin; Niemegeer, C.J.E., Verbruggen, F.J., Janssen, P.A.J., Effect of various drugs in carrageenan-induced oedema in the rat hind paw (1964) The J Pharmacy and Pharmacology, 16, p. 810",
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Analgesic and antiinflammatory activity of leaf extracts of Commiphora caudata in rodents. / Mohan, C.G.; Gokavi, S.; Viswanatha, G.L.; Shylaja, H.; Nandakumar, K.

In: Pharmacologyonline, Vol. 2, 2009, p. 991-998.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analgesic and antiinflammatory activity of leaf extracts of Commiphora caudata in rodents

AU - Mohan, C.G.

AU - Gokavi, S.

AU - Viswanatha, G.L.

AU - Shylaja, H.

AU - Nandakumar, K.

N1 - Cited By :2 Export Date: 10 November 2017 Correspondence Address: Viswanatha, G.L.; Preclinical Pharmacology and Toxicology, Connexios Life Sciences Pvt Ltd, J.P. Nagar, Bangalore, India; email: glv_000@yahoo.com Chemicals/CAS: alcohol, 64-17-5; carrageenan, 9000-07-1, 9049-05-2, 9061-82-9, 9064-57-7; diclofenac, 15307-79-6, 15307-86-5; formaldehyde, 50-00-0; pentazocine, 359-83-1, 64024-15-3; water, 7732-18-5 Manufacturers: Cadila, India; Novartis, United States References: Ritter, J.M., Anti-inflammatory and immunosuppressive drugs (2000) Pharmacology, pp. 229-232. , edited by Rang HP, Dale MM, Churchill Livingston, Edinburgh; Haslett C, Chilvers ER, Boon NA, Colledge NR, Hunter JA. Side effects of NSAIDS.In: Davidson's Principles and Practice of Medicine. Churchill Livingstone, Edinburgh 2002:989-990; Shanmugasundaram, P., Venkataraman, S., Antinociceptive activity of Hygrophila auriculata (schum) heine (2005) Afr J Trad Complem Alter Med, 2, pp. 62-69; Prajapathi, Purohit, Sharma, Kumar. In: A hand book of medicinal plants Jodhpur:Dr.Upadesh purohit for Agrobios (India):2003.p.306-7 (sec II); (1996) Indian Materia Medica, , Nadkarni KM, Nadkarni AK, Editors, Bombay; Popular prakashan; Deepa, V.S., Kumar, P.S., Latha, S., Selvamani, P., Srinivasan, S., Antioxidant studies on the ethanolic extract of Commiphora spp (2009) African Journal of Biotechnology, 8 (8), pp. 1630-1636; OECD 2001-gudeline on acute oral toxicity (AOT) Environmental health and safety monograph series on testing and adjustment No.425; Dubuisson, D., Dennis, S.G., The formalin test : A quantitative study of the analgesic effects of morphine, epedrine, and brain stem stimulation in rats and cats (1977) Pain, 4, p. 161; Shibata, M., Ohkubo, T., Takahashi, H., Inoki, R., Modified formalin test characteristic biphasic pain response (1989) Pain, 38, p. 347; Rosland, J.H., Tjoisen, A., Maehle, B., Hole, K., The formalin test in mice:effect of formalin concentration (1990) Pain, 42, p. 235; Vogel, G.H., Vogel, W.H., Scholkens, B.A., Sandow, J., Muller, G., Vogel, W.F., Pharmacological assays (2002) Drug discovery and evaluation, pp. 725-772. , 2nd ed. Springer-Verlag, Berlin; Niemegeer, C.J.E., Verbruggen, F.J., Janssen, P.A.J., Effect of various drugs in carrageenan-induced oedema in the rat hind paw (1964) The J Pharmacy and Pharmacology, 16, p. 810

PY - 2009

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N2 - The present study was undertaken to evaluate the analgesic and anti-inflammatory activity of Alcoholic (70%) and Aqueos extracts of leaves of Commiphora caudata in rodents. The dried powder of the leaves was initially extracted with 70% Alcoholic (ALCC) and Water (AQCC) successively. Acute oral toxicity of ALCC and AQCC were conducted as per OECD guidelines 425. Acute toxicity studies revealed that both the extracts are safe upto 2000mg/kg.The extracts were evaluated for Analgesic activity with formalin induced paw licking test in mice using Pentazocin (2mg/kg,i.p.) as a standard and Anti-inflammatory activity was evaluated with carrageenan induced rat paw edema model in wistar rats using Diclofenac (15mg/kg,i.p) a standard. Acute toxicity results have revealed that both ALCC and AQCC were safe upto 2000 mg/kg dose. In formalin induced paw licking test ALCC at 100, 200 and 400mg/kg, AQCC at 200 and 400mg/kg have showed significant inhibition of paw licking in both early and late phase of the formalin test when compare to control group; In carrageenan induced rat paw edema model ALCC at 200 and 400mg/ kg, AQCC at 400mg/kg have showed significant inhibition in carrageenan induced rat paw when compare to control group, these observations suggests that ALCC and AQCC possess good analgesic and antiinflammatory activity but the Alcoholic is more potent when compare to Aqueous extract.

AB - The present study was undertaken to evaluate the analgesic and anti-inflammatory activity of Alcoholic (70%) and Aqueos extracts of leaves of Commiphora caudata in rodents. The dried powder of the leaves was initially extracted with 70% Alcoholic (ALCC) and Water (AQCC) successively. Acute oral toxicity of ALCC and AQCC were conducted as per OECD guidelines 425. Acute toxicity studies revealed that both the extracts are safe upto 2000mg/kg.The extracts were evaluated for Analgesic activity with formalin induced paw licking test in mice using Pentazocin (2mg/kg,i.p.) as a standard and Anti-inflammatory activity was evaluated with carrageenan induced rat paw edema model in wistar rats using Diclofenac (15mg/kg,i.p) a standard. Acute toxicity results have revealed that both ALCC and AQCC were safe upto 2000 mg/kg dose. In formalin induced paw licking test ALCC at 100, 200 and 400mg/kg, AQCC at 200 and 400mg/kg have showed significant inhibition of paw licking in both early and late phase of the formalin test when compare to control group; In carrageenan induced rat paw edema model ALCC at 200 and 400mg/ kg, AQCC at 400mg/kg have showed significant inhibition in carrageenan induced rat paw when compare to control group, these observations suggests that ALCC and AQCC possess good analgesic and antiinflammatory activity but the Alcoholic is more potent when compare to Aqueous extract.

M3 - Article

VL - 2

SP - 991

EP - 998

JO - Pharmacologyonline

JF - Pharmacologyonline

SN - 1827-8620

ER -