Analysis of flow cytometric enumeration of CD34+ cells in leukapheresis product from South Indian cancer centre

Mohandoss Murugesan, Chandran K. Nair, Sangeetha K. Nayanar, Kalyana Chakravarthy Pentapati

Research output: Contribution to journalArticle

Abstract

Background: The success of peripheral blood stem cell (PBSC) transplant often relies on CD34+ cells counting. As the assay needs to be repeated in duplicate, this study was conducted to observe the variations between the duplicates with respect to concentration of leukapheresis product. Methods: The assay followed was single platform method - ISHAGE protocol published by the International Society of Haematotherapy and Graft Engineering. New parameter named variation index was used to compare the difference between the duplicates. Results: We present our data on 111 samples collected from leukapheresis product. 10% of the assay did not meet the criteria and required repeat testing. We observed no significant difference betweenthe duplicate samples with respect to low (CD34+ cells/μl < 500; P=0.632), Medium: 500 to 1000 CD34+ cells/μl, P=0.085 and High > 1000 CD34+ cells/μl, P=0.864). Conclusion: Testing the CD34 enumeration in duplicate and verifying its repeatability for variation index of less than 10 will help in accurately determining CD34+ dose calculations.

Original languageEnglish
Article number7
JournalOnline Journal of Health and Allied Sciences
Volume18
Issue number1
Publication statusPublished - 01-01-2019

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Leukapheresis
Transplants
Neoplasms

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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title = "Analysis of flow cytometric enumeration of CD34+ cells in leukapheresis product from South Indian cancer centre",
abstract = "Background: The success of peripheral blood stem cell (PBSC) transplant often relies on CD34+ cells counting. As the assay needs to be repeated in duplicate, this study was conducted to observe the variations between the duplicates with respect to concentration of leukapheresis product. Methods: The assay followed was single platform method - ISHAGE protocol published by the International Society of Haematotherapy and Graft Engineering. New parameter named variation index was used to compare the difference between the duplicates. Results: We present our data on 111 samples collected from leukapheresis product. 10{\%} of the assay did not meet the criteria and required repeat testing. We observed no significant difference betweenthe duplicate samples with respect to low (CD34+ cells/μl < 500; P=0.632), Medium: 500 to 1000 CD34+ cells/μl, P=0.085 and High > 1000 CD34+ cells/μl, P=0.864). Conclusion: Testing the CD34 enumeration in duplicate and verifying its repeatability for variation index of less than 10 will help in accurately determining CD34+ dose calculations.",
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Analysis of flow cytometric enumeration of CD34+ cells in leukapheresis product from South Indian cancer centre. / Murugesan, Mohandoss; Nair, Chandran K.; Nayanar, Sangeetha K.; Pentapati, Kalyana Chakravarthy.

In: Online Journal of Health and Allied Sciences, Vol. 18, No. 1, 7, 01.01.2019.

Research output: Contribution to journalArticle

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T1 - Analysis of flow cytometric enumeration of CD34+ cells in leukapheresis product from South Indian cancer centre

AU - Murugesan, Mohandoss

AU - Nair, Chandran K.

AU - Nayanar, Sangeetha K.

AU - Pentapati, Kalyana Chakravarthy

PY - 2019/1/1

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N2 - Background: The success of peripheral blood stem cell (PBSC) transplant often relies on CD34+ cells counting. As the assay needs to be repeated in duplicate, this study was conducted to observe the variations between the duplicates with respect to concentration of leukapheresis product. Methods: The assay followed was single platform method - ISHAGE protocol published by the International Society of Haematotherapy and Graft Engineering. New parameter named variation index was used to compare the difference between the duplicates. Results: We present our data on 111 samples collected from leukapheresis product. 10% of the assay did not meet the criteria and required repeat testing. We observed no significant difference betweenthe duplicate samples with respect to low (CD34+ cells/μl < 500; P=0.632), Medium: 500 to 1000 CD34+ cells/μl, P=0.085 and High > 1000 CD34+ cells/μl, P=0.864). Conclusion: Testing the CD34 enumeration in duplicate and verifying its repeatability for variation index of less than 10 will help in accurately determining CD34+ dose calculations.

AB - Background: The success of peripheral blood stem cell (PBSC) transplant often relies on CD34+ cells counting. As the assay needs to be repeated in duplicate, this study was conducted to observe the variations between the duplicates with respect to concentration of leukapheresis product. Methods: The assay followed was single platform method - ISHAGE protocol published by the International Society of Haematotherapy and Graft Engineering. New parameter named variation index was used to compare the difference between the duplicates. Results: We present our data on 111 samples collected from leukapheresis product. 10% of the assay did not meet the criteria and required repeat testing. We observed no significant difference betweenthe duplicate samples with respect to low (CD34+ cells/μl < 500; P=0.632), Medium: 500 to 1000 CD34+ cells/μl, P=0.085 and High > 1000 CD34+ cells/μl, P=0.864). Conclusion: Testing the CD34 enumeration in duplicate and verifying its repeatability for variation index of less than 10 will help in accurately determining CD34+ dose calculations.

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