Analysis of reticulin fiber pattern in lymph nodes with metastasis from oral squamous cell carcinoma

Shanmukha Raviteja Yinti, Srikant Natarajan, Karen Boaz, Amitha J. Lewis, Jay Pandya, Supriya Nikita Kapila

Research output: Contribution to journalArticle

Abstract

Introduction: The aim of the study was to determine the susceptibility of lymph nodes to metastasis in oral squamous cell carcinoma (OSCC) by analyzing the alterations in reticulin fiber pattern. Materials and Methods: This retrospective study on 30 cases of OSCC had 15 cases that presented with lymph node metastasis (test group) and 15 cases without metastatic episodes (control group). Four micron thick sections of the tumor proper and the resected lymph nodes of the cases were stained with hematoxylin and eosin, and Gordon and Sweet's stain for reticulin. Statistical analysis of the variations in the staining patterns of reticulin at the invasive tumor front (ITF), involved and uninvolved lymph nodes were done with Statistical Package for Social Sciences (SPSS) 15 version using chi-square test. Results: The assessment of reticulin fiber patterns at the ITF, cortical and medullary areas of lymph nodes in test cases showed thin, long individual fibers, as compared to thick, shorter interlacing fibers that were seen in control cases. The peritumoral and uninvolved areas in metastatic lymph nodes of almost all test cases showed very scant fibers. Conclusion: Reticulin pattern alteration by aggressive tumors may be appreciated as a part of the wide spectrum of "proneoplastic" stromal alterations. The histopathologist can discern these changes and thereby aid the clinician in predicting tumor behavior, the clinical course of the disease, and weighing the prognostic implications.

Original languageEnglish
Pages (from-to)104-108
Number of pages5
JournalDental Hypotheses
Volume6
Issue number3
DOIs
Publication statusPublished - 01-07-2015

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Reticulin
Squamous Cell Carcinoma
Lymph Nodes
Neoplasm Metastasis
Neoplasms
Social Sciences
Chi-Square Distribution
Hematoxylin
Eosine Yellowish-(YS)
Coloring Agents
Retrospective Studies
Staining and Labeling
Control Groups

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Yinti, Shanmukha Raviteja ; Natarajan, Srikant ; Boaz, Karen ; Lewis, Amitha J. ; Pandya, Jay ; Kapila, Supriya Nikita. / Analysis of reticulin fiber pattern in lymph nodes with metastasis from oral squamous cell carcinoma. In: Dental Hypotheses. 2015 ; Vol. 6, No. 3. pp. 104-108.
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abstract = "Introduction: The aim of the study was to determine the susceptibility of lymph nodes to metastasis in oral squamous cell carcinoma (OSCC) by analyzing the alterations in reticulin fiber pattern. Materials and Methods: This retrospective study on 30 cases of OSCC had 15 cases that presented with lymph node metastasis (test group) and 15 cases without metastatic episodes (control group). Four micron thick sections of the tumor proper and the resected lymph nodes of the cases were stained with hematoxylin and eosin, and Gordon and Sweet's stain for reticulin. Statistical analysis of the variations in the staining patterns of reticulin at the invasive tumor front (ITF), involved and uninvolved lymph nodes were done with Statistical Package for Social Sciences (SPSS) 15 version using chi-square test. Results: The assessment of reticulin fiber patterns at the ITF, cortical and medullary areas of lymph nodes in test cases showed thin, long individual fibers, as compared to thick, shorter interlacing fibers that were seen in control cases. The peritumoral and uninvolved areas in metastatic lymph nodes of almost all test cases showed very scant fibers. Conclusion: Reticulin pattern alteration by aggressive tumors may be appreciated as a part of the wide spectrum of {"}proneoplastic{"} stromal alterations. The histopathologist can discern these changes and thereby aid the clinician in predicting tumor behavior, the clinical course of the disease, and weighing the prognostic implications.",
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Analysis of reticulin fiber pattern in lymph nodes with metastasis from oral squamous cell carcinoma. / Yinti, Shanmukha Raviteja; Natarajan, Srikant; Boaz, Karen; Lewis, Amitha J.; Pandya, Jay; Kapila, Supriya Nikita.

In: Dental Hypotheses, Vol. 6, No. 3, 01.07.2015, p. 104-108.

Research output: Contribution to journalArticle

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AB - Introduction: The aim of the study was to determine the susceptibility of lymph nodes to metastasis in oral squamous cell carcinoma (OSCC) by analyzing the alterations in reticulin fiber pattern. Materials and Methods: This retrospective study on 30 cases of OSCC had 15 cases that presented with lymph node metastasis (test group) and 15 cases without metastatic episodes (control group). Four micron thick sections of the tumor proper and the resected lymph nodes of the cases were stained with hematoxylin and eosin, and Gordon and Sweet's stain for reticulin. Statistical analysis of the variations in the staining patterns of reticulin at the invasive tumor front (ITF), involved and uninvolved lymph nodes were done with Statistical Package for Social Sciences (SPSS) 15 version using chi-square test. Results: The assessment of reticulin fiber patterns at the ITF, cortical and medullary areas of lymph nodes in test cases showed thin, long individual fibers, as compared to thick, shorter interlacing fibers that were seen in control cases. The peritumoral and uninvolved areas in metastatic lymph nodes of almost all test cases showed very scant fibers. Conclusion: Reticulin pattern alteration by aggressive tumors may be appreciated as a part of the wide spectrum of "proneoplastic" stromal alterations. The histopathologist can discern these changes and thereby aid the clinician in predicting tumor behavior, the clinical course of the disease, and weighing the prognostic implications.

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