Analytical and bioanalytical HPLC method for simultaneous estimation of 5-fluorouracil and sonidegib

Bharath S. Padya, Aswathi R. Hegde, Sadhana P. Mutalik, Swati Biswas, Srinivas Mutalik

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: To develop a new sensitive RP-HPLC method for simultaneous estimation of 5-fluorouracil (5-FU) and sonidegib (SDG). Materials & methods: Analytical and bioanalytical methods for simultaneous quantification of 5-FU and SDG in bulk, nanoformulations and in rat plasma were developed and validated using a gradient elution technique. Results: Separation of the analytes was effected on a Luna® C18 LC column using a mobile mixture comprising acetonitrile and acidified water. 5-FU and SDG were extracted from plasma matrix using liquid-liquid extraction. The applicability of the method was verified through single-dose oral pharmacokinetic study in Wistar rats. Conclusion: The developed methods allow a specific, sensitive and steady analytical procedure for the simultaneous estimation of 5-FU and SDG in nanoformulations and biological matrix.

Original languageEnglish
Pages (from-to)29-45
Number of pages17
JournalBioanalysis
Volume14
Issue number1
DOIs
Publication statusPublished - 01-2022

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

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