A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for aspirin in presence of its degradation products is developed and validated. The method employed Hypersil BDS C 18 (100 x 4.6 mm 5μ) column as stationary phase.The mobile phase consisted of sodium perchlorate buffer (pH 2.5): acetonitrile: isopropyl alcohol (85:14:1 % v/v). It is pumped through the chromatographic system at a flow rate of 1.5 ml min-1.The UV detector is operated at 275 nm. This system was found to give good resolution between aspirin and its degradation products. Method was validated as per ICH guidelines.
|Number of pages||11|
|Journal||International Journal of ChemTech Research|
|Publication status||Published - 01-2010|
All Science Journal Classification (ASJC) codes
- Chemical Engineering(all)