Analytical method development and validation for aspirin

S. Suresh Kumar, Latif D. Jamadar, Krishnamurthy Bhat, Prashant B. Musmade, S. G. Vasantharaju, N. Udupa

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for aspirin in presence of its degradation products is developed and validated. The method employed Hypersil BDS C 18 (100 x 4.6 mm 5μ) column as stationary phase.The mobile phase consisted of sodium perchlorate buffer (pH 2.5): acetonitrile: isopropyl alcohol (85:14:1 % v/v). It is pumped through the chromatographic system at a flow rate of 1.5 ml min-1.The UV detector is operated at 275 nm. This system was found to give good resolution between aspirin and its degradation products. Method was validated as per ICH guidelines.

Original languageEnglish
Pages (from-to)389-399
Number of pages11
JournalInternational Journal of ChemTech Research
Volume2
Issue number1
Publication statusPublished - 01-2010

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Aspirin
Ultraviolet detectors
Degradation
2-Propanol
Acetonitrile
Buffers
Alcohols
Sodium
Flow rate
Liquids
Costs
sodium perchlorate
acetonitrile

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Suresh Kumar, S. ; Jamadar, Latif D. ; Bhat, Krishnamurthy ; Musmade, Prashant B. ; Vasantharaju, S. G. ; Udupa, N. / Analytical method development and validation for aspirin. In: International Journal of ChemTech Research. 2010 ; Vol. 2, No. 1. pp. 389-399.
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Analytical method development and validation for aspirin. / Suresh Kumar, S.; Jamadar, Latif D.; Bhat, Krishnamurthy; Musmade, Prashant B.; Vasantharaju, S. G.; Udupa, N.

In: International Journal of ChemTech Research, Vol. 2, No. 1, 01.2010, p. 389-399.

Research output: Contribution to journalArticle

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