Analytical method development and validation for dipyridamole

S. Suresh Kumar, Latif D. Jamadar, Krishnamurthy Bhat, P. C. Jagdish, Shriram Pathak, N. Udupa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for dipyridamole in presence of its degradation products is developed and validated. The method employed Targa C8 column i.e., (250 × 4.6 mm 5 μm particle size) column as stationary phase. The mobile phase consists of acetonitrile and pH3.0 buffer in the ratio of 35:65%. It is pumped through the chromatographic system at a flow rate of 1.2 ml/min. The UV detector is operated at 282 nm. This system was found to give good resolution between dipyridamole and its degradation products. Method was validated as per ICH guidelines.

Original languageEnglish
Pages (from-to)333-338
Number of pages6
JournalJournal of Pharmaceutical Research and Health Care
Volume2
Issue number4
Publication statusPublished - 10-2010

Fingerprint

Dipyridamole
Particle Size
Buffers
High Pressure Liquid Chromatography
Guidelines
Costs and Cost Analysis

All Science Journal Classification (ASJC) codes

  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

@article{c068337da66d48eaa136fb1b830cef25,
title = "Analytical method development and validation for dipyridamole",
abstract = "A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for dipyridamole in presence of its degradation products is developed and validated. The method employed Targa C8 column i.e., (250 × 4.6 mm 5 μm particle size) column as stationary phase. The mobile phase consists of acetonitrile and pH3.0 buffer in the ratio of 35:65{\%}. It is pumped through the chromatographic system at a flow rate of 1.2 ml/min. The UV detector is operated at 282 nm. This system was found to give good resolution between dipyridamole and its degradation products. Method was validated as per ICH guidelines.",
author = "{Suresh Kumar}, S. and Jamadar, {Latif D.} and Krishnamurthy Bhat and Jagdish, {P. C.} and Shriram Pathak and N. Udupa",
year = "2010",
month = "10",
language = "English",
volume = "2",
pages = "333--338",
journal = "Journal of Pharmaceutical Research and Health Care",
issn = "2250-1444",
publisher = "Journal of Pharmaceutical Research and Health Care",
number = "4",

}

Analytical method development and validation for dipyridamole. / Suresh Kumar, S.; Jamadar, Latif D.; Bhat, Krishnamurthy; Jagdish, P. C.; Pathak, Shriram; Udupa, N.

In: Journal of Pharmaceutical Research and Health Care, Vol. 2, No. 4, 10.2010, p. 333-338.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analytical method development and validation for dipyridamole

AU - Suresh Kumar, S.

AU - Jamadar, Latif D.

AU - Bhat, Krishnamurthy

AU - Jagdish, P. C.

AU - Pathak, Shriram

AU - Udupa, N.

PY - 2010/10

Y1 - 2010/10

N2 - A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for dipyridamole in presence of its degradation products is developed and validated. The method employed Targa C8 column i.e., (250 × 4.6 mm 5 μm particle size) column as stationary phase. The mobile phase consists of acetonitrile and pH3.0 buffer in the ratio of 35:65%. It is pumped through the chromatographic system at a flow rate of 1.2 ml/min. The UV detector is operated at 282 nm. This system was found to give good resolution between dipyridamole and its degradation products. Method was validated as per ICH guidelines.

AB - A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for dipyridamole in presence of its degradation products is developed and validated. The method employed Targa C8 column i.e., (250 × 4.6 mm 5 μm particle size) column as stationary phase. The mobile phase consists of acetonitrile and pH3.0 buffer in the ratio of 35:65%. It is pumped through the chromatographic system at a flow rate of 1.2 ml/min. The UV detector is operated at 282 nm. This system was found to give good resolution between dipyridamole and its degradation products. Method was validated as per ICH guidelines.

UR - http://www.scopus.com/inward/record.url?scp=78650259042&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650259042&partnerID=8YFLogxK

M3 - Article

VL - 2

SP - 333

EP - 338

JO - Journal of Pharmaceutical Research and Health Care

JF - Journal of Pharmaceutical Research and Health Care

SN - 2250-1444

IS - 4

ER -