Analytical method development and validation for dipyridamole

S. Suresh Kumar; Latif D. Jamadar; Krishnamurthy Bhat; P. C. Jagdish; Shriram Pathak; N. Udupa

Analytical method development and validation for dipyridamole

S. Suresh Kumar, Latif D. Jamadar, Krishnamurthy Bhat, P. C. Jagdish, Shriram Pathak, N. Udupa

Research output: Contribution to journal › Article › peer-review

Abstract

A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for dipyridamole in presence of its degradation products is developed and validated. The method employed Targa C₈ column i.e., (250 × 4.6 mm 5 μm particle size) column as stationary phase. The mobile phase consists of acetonitrile and pH3.0 buffer in the ratio of 35:65%. It is pumped through the chromatographic system at a flow rate of 1.2 ml/min. The UV detector is operated at 282 nm. This system was found to give good resolution between dipyridamole and its degradation products. Method was validated as per ICH guidelines.

Original language	English
Pages (from-to)	333-338
Number of pages	6
Journal	Journal of Pharmaceutical Research and Health Care
Volume	2
Issue number	4
Publication status	Published - 10-2010

All Science Journal Classification (ASJC) codes

Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

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title = "Analytical method development and validation for dipyridamole",

abstract = "A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for dipyridamole in presence of its degradation products is developed and validated. The method employed Targa C8 column i.e., (250 × 4.6 mm 5 μm particle size) column as stationary phase. The mobile phase consists of acetonitrile and pH3.0 buffer in the ratio of 35:65%. It is pumped through the chromatographic system at a flow rate of 1.2 ml/min. The UV detector is operated at 282 nm. This system was found to give good resolution between dipyridamole and its degradation products. Method was validated as per ICH guidelines.",

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T1 - Analytical method development and validation for dipyridamole

AU - Suresh Kumar, S.

AU - Jamadar, Latif D.

AU - Bhat, Krishnamurthy

AU - Jagdish, P. C.

AU - Pathak, Shriram

AU - Udupa, N.

PY - 2010/10

Y1 - 2010/10

N2 - A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for dipyridamole in presence of its degradation products is developed and validated. The method employed Targa C8 column i.e., (250 × 4.6 mm 5 μm particle size) column as stationary phase. The mobile phase consists of acetonitrile and pH3.0 buffer in the ratio of 35:65%. It is pumped through the chromatographic system at a flow rate of 1.2 ml/min. The UV detector is operated at 282 nm. This system was found to give good resolution between dipyridamole and its degradation products. Method was validated as per ICH guidelines.

AB - A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for dipyridamole in presence of its degradation products is developed and validated. The method employed Targa C8 column i.e., (250 × 4.6 mm 5 μm particle size) column as stationary phase. The mobile phase consists of acetonitrile and pH3.0 buffer in the ratio of 35:65%. It is pumped through the chromatographic system at a flow rate of 1.2 ml/min. The UV detector is operated at 282 nm. This system was found to give good resolution between dipyridamole and its degradation products. Method was validated as per ICH guidelines.

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JF - Journal of Pharmaceutical Research and Health Care

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Analytical method development and validation for dipyridamole

Abstract

All Science Journal Classification (ASJC) codes

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