Analytical Method Development and Validation for HPLC-ECD Determination of Moxifloxacin in Marketed Formulations

G. Phani Sekhar Reddy, K. S. Navyasree, P. C. Jagadish, Krishnamurthy Bhat

Research output: Contribution to journalArticle

Abstract

An HPLC-ECD analytical method with high reproducibility and wide linearity range has been developed and validated. Moxifloxacin was separated and identified using this method with a simple mobile phase comprising Britton Robinson buffer pH 5.0 and methanol (93: 7 v/v) flowing at a rate of 0.5 mL/min through Acclaim C18 column (150 mm × 4.6 mm × 5 im) maintained at 35°C and detected at redox potential value of 1.0 V. The LOD and LOQ were found to be 2.2 and 6.6 μg/mL, respectively. The HPLC-ECD method of moxifloxacin analysis was validated as per ICH Q2R1 guidelines and applied for assay of marketed moxifloxacin formulations to establish the acceptable recovery.

Original languageEnglish
Pages (from-to)674-679
Number of pages6
JournalPharmaceutical Chemistry Journal
Volume52
Issue number7
DOIs
Publication statusPublished - 01-10-2018

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High Pressure Liquid Chromatography
Oxidation-Reduction
Methanol
Buffers
Guidelines
moxifloxacin

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

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title = "Analytical Method Development and Validation for HPLC-ECD Determination of Moxifloxacin in Marketed Formulations",
abstract = "An HPLC-ECD analytical method with high reproducibility and wide linearity range has been developed and validated. Moxifloxacin was separated and identified using this method with a simple mobile phase comprising Britton Robinson buffer pH 5.0 and methanol (93: 7 v/v) flowing at a rate of 0.5 mL/min through Acclaim C18 column (150 mm × 4.6 mm × 5 im) maintained at 35°C and detected at redox potential value of 1.0 V. The LOD and LOQ were found to be 2.2 and 6.6 μg/mL, respectively. The HPLC-ECD method of moxifloxacin analysis was validated as per ICH Q2R1 guidelines and applied for assay of marketed moxifloxacin formulations to establish the acceptable recovery.",
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Analytical Method Development and Validation for HPLC-ECD Determination of Moxifloxacin in Marketed Formulations. / Phani Sekhar Reddy, G.; Navyasree, K. S.; Jagadish, P. C.; Bhat, Krishnamurthy.

In: Pharmaceutical Chemistry Journal, Vol. 52, No. 7, 01.10.2018, p. 674-679.

Research output: Contribution to journalArticle

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