Analytical Method Development and Validation for HPLC-ECD Determination of Moxifloxacin in Marketed Formulations

G. Phani Sekhar Reddy, K. S. Navyasree, P. C. Jagadish, Krishnamurthy Bhat

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An HPLC-ECD analytical method with high reproducibility and wide linearity range has been developed and validated. Moxifloxacin was separated and identified using this method with a simple mobile phase comprising Britton Robinson buffer pH 5.0 and methanol (93: 7 v/v) flowing at a rate of 0.5 mL/min through Acclaim C18 column (150 mm × 4.6 mm × 5 im) maintained at 35°C and detected at redox potential value of 1.0 V. The LOD and LOQ were found to be 2.2 and 6.6 μg/mL, respectively. The HPLC-ECD method of moxifloxacin analysis was validated as per ICH Q2R1 guidelines and applied for assay of marketed moxifloxacin formulations to establish the acceptable recovery.

Original languageEnglish
Pages (from-to)674-679
Number of pages6
JournalPharmaceutical Chemistry Journal
Issue number7
Publication statusPublished - 01-10-2018


All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

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