Anti-osteoporotic activity of the petroleum ether extract of Cissus quadrangularis Linn. in ovariectomized wistar rats

Bhagath Kumar Potu, Muddanna S. Rao, Gopalan Kutty Nampurath, Mallikarjuna Rao Chamallamudi, Soubhagya Ranjan Nayak, Huban Thomas

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Abstract

Background: The plant Cissus quadrangularis (CQ) is used as an osteoprotective agent in Ayurveda, the Indian system of alternative medicine. The present study was done to validate the anti-osteoporotic role of the petroleum ether extract of CQ on ovariectomy-induced osteoporosis in rats. Methods: Female wistar rats were divided into five groups of six rats each; (1) The Normal control (NC) group (n = 6) received no surgery and no treatment, (2). The Sham control (SHAM) group (n = 6) received sham surgery but no treatment, (3) the Ovariectomized (OVX) group (n = 6) received an ovariectomy and normal saline treatment for 90 days, (4) the Ovariectomized + raloxifene (OVX + RAL) group (n = 6) received an ovariectomy and treatment with raloxifene, a known anti-osteoporotic agent for 90 days commencing from 22nd post ovariectomy day and (5) the Ovariectomy + Cissus quadrangularis (OVX + CQ) group (n = 6) received an ovariectomy, and treatment with the petroleum ether extract of CQ 500 mg/kg body weight daily for 90 days commencing from 22nd post ovariectomy day. At the end of the treatment period, rats in all groups were sacrificed and the right femur was used for biomechanical analysis, and the left femur for histomorphometrical analysis. Results: CQ significantly increased the force required to break the femur (p <0.001) and significantly increased the thickness of both cortical (p <0.001) and trabecular bone (p <0.001). This action of CQ was comparable to the action of raloxifene. Conclusions:The petroleum ether extract of CQ stem seems to possess anti-osteoporotic activity in rats.
Original languageEnglish
Pages (from-to)252-257
Number of pages6
JournalChang Gung Medical Journal
Volume33
Issue number3
Publication statusPublished - 2010

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Cissus
Ovariectomy
Wistar Rats
Femur
Therapeutics
Control Groups
naphtha
Complementary Therapies
Systems Analysis
Osteoporosis
Body Weight

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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Potu, Bhagath Kumar ; Rao, Muddanna S. ; Nampurath, Gopalan Kutty ; Chamallamudi, Mallikarjuna Rao ; Nayak, Soubhagya Ranjan ; Thomas, Huban. / Anti-osteoporotic activity of the petroleum ether extract of Cissus quadrangularis Linn. in ovariectomized wistar rats. In: Chang Gung Medical Journal. 2010 ; Vol. 33, No. 3. pp. 252-257.
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title = "Anti-osteoporotic activity of the petroleum ether extract of Cissus quadrangularis Linn. in ovariectomized wistar rats",
abstract = "Background: The plant Cissus quadrangularis (CQ) is used as an osteoprotective agent in Ayurveda, the Indian system of alternative medicine. The present study was done to validate the anti-osteoporotic role of the petroleum ether extract of CQ on ovariectomy-induced osteoporosis in rats. Methods: Female wistar rats were divided into five groups of six rats each; (1) The Normal control (NC) group (n = 6) received no surgery and no treatment, (2). The Sham control (SHAM) group (n = 6) received sham surgery but no treatment, (3) the Ovariectomized (OVX) group (n = 6) received an ovariectomy and normal saline treatment for 90 days, (4) the Ovariectomized + raloxifene (OVX + RAL) group (n = 6) received an ovariectomy and treatment with raloxifene, a known anti-osteoporotic agent for 90 days commencing from 22nd post ovariectomy day and (5) the Ovariectomy + Cissus quadrangularis (OVX + CQ) group (n = 6) received an ovariectomy, and treatment with the petroleum ether extract of CQ 500 mg/kg body weight daily for 90 days commencing from 22nd post ovariectomy day. At the end of the treatment period, rats in all groups were sacrificed and the right femur was used for biomechanical analysis, and the left femur for histomorphometrical analysis. Results: CQ significantly increased the force required to break the femur (p <0.001) and significantly increased the thickness of both cortical (p <0.001) and trabecular bone (p <0.001). This action of CQ was comparable to the action of raloxifene. Conclusions:The petroleum ether extract of CQ stem seems to possess anti-osteoporotic activity in rats.",
author = "Potu, {Bhagath Kumar} and Rao, {Muddanna S.} and Nampurath, {Gopalan Kutty} and Chamallamudi, {Mallikarjuna Rao} and Nayak, {Soubhagya Ranjan} and Huban Thomas",
note = "Cited By :18 Export Date: 10 November 2017 CODEN: CIHCE Correspondence Address: Rao, M. S.; Department of Anatomy, Faculty of Medicine, Kuwait University, P.O. box: 24923, Safat, 13110, Kuwait; email: muddannas@yahoo.com Chemicals/CAS: petroleum ether, 8032-32-4; raloxifene, 82640-04-8, 84449-90-1; Plant Extracts; Raloxifene References: Potu, B.K., Rao, M.S., Swamy, N.V.B., Kutty, G.N., Cissus quadrangularis plant extract enhances the ossification of fetal bones (2007) Pharmacologyonline, 1, pp. 63-70; Rao, M.S., Potu, B.K., Swamy, N.V.B., Kutty, G.N., Cissus Quadrangularis plant extract enhances the development of cortical bone and trabeculae in the fetal femur (2007) Pharmacologyonline, 3, pp. 190-202; Potu, B.K., Rao, M.S., Kutty, G.N., Bhat, M.R.K., Chamallamudi, M.R., Nayak, S.R., Petroleum ether extract of Cissus quadrangularis (Linn) stimulates the growth of fetal bone during intrauterine developmental period: A morphometric analysis (2008) Clinics, 63, pp. 815-820. , (Sao Paulo); Shirwaikar, A., Khan, S., Malini, S., Antiosteoporotic effect of ethanol extract of Cissus quadrangularis Linn. on ovariectomized rat (2003) J Ethnopharmacol, 89, pp. 245-250; Potu, B.K., Bhat, K.M., Rao, M.S., Nampurath, G.K., Chamallamudi, M.R., Nayak, S.R., Petroleum ether extract of Cissus quadrangularis (Linn.) enhances bone marrow mesenchymal stem cell proliferation and facilitates osteoblastogenesis (2009) Clinics, 64, pp. 993-998; Ghosh, M.N., (1984) Fundamentals of Experimental Pharmacology, pp. 153-157. , 2nd ed. Calcutta, India: Scientific Book Agency; Joffe, I., Katz, I., Jacobs, T., Stein, B., Takizawa, M., Liu, C., Berlin, J., Epstein, S., 17 beta-estradiol prevents osteopenia in the oophorectomized rat treated with cyclosporin-A (1992) Endocrinology, 130, pp. 1578-1586; Cheung, J., Mak, Y.T., Papaioannou, S., Evans, B.A.J., Fogelman, I., Hampson, G., Interleukin-6 (IL-6), IL-1, receptor activator of nuclear factor kB ligand (RANKL) and osteoprotegerin production by human osteoblastic cells: Comparison of the effects of 17-beta oestrodiol and raloxifene (2003) J Endocrinol, 177, pp. 423-433; Peng, Z., Tuukkanen, J., Zhang, H., The mechanical strength of bone in different rat models of experimental osteoporosis (1994) Bone, 15, pp. 523-532; Miao, D., Scutt, A., Histochemical localization of alkaline phosphatase activity in decalcified bone and cartilage (2002) J Histochem Cytochem, 50, pp. 333-340; Sen, S.P., Studies on the active constituents of Cissus quadrangularis Wall (1966) Curr Sci, 35, p. 317; Singh, G., Rawat, P., Maurya, R., Constituents of Cissus quadrangularis (2007) Nat Prod Res, 21, pp. 522-528; Bhutani, K.K., Kapoor, R., Atal, C.K., Two unsymmetric tetracyclic triterpenoids from Cissus quadrangularis (1984) Phytochemistry, 23, pp. 407-410; Gupta, M.M., Verma, R.K., Unsymmetric tetracyclic triterpenoids from Cissus quadrangularis (1990) Phytochemistry, 29, pp. 336-337; Mehta, M., Kaur, N., Bhutani, K.K., Determination of marker constituents from Cissus quadiangularis Linn. and their quantitation by HPTLC and HPLC (2001) Phytochem Anal, 12, pp. 91-95; Kausch, A.P., Horner, H.T., A comparison of calcium oxalate crystals isolated from callus cultures and their explant sources (1982) Scan Electron Microsc, 1, pp. 199-211; Gupta, M.M., Verma, R.K., Lipid constituents of Cissus quadrangularis (1991) Phytochemistry, 30, pp. 875-878; Adesanya, S.A., Nia, R., Maartin, M.T., Boukamcha, N., Montagnac, A., Pais, M., Stilbene derivatives from Cissus quadrangularis (1999) J Nat Prod, 62, pp. 1694-1695; Pluemjai, T., Saifah, E., Constituents of Cissus quadrangularis Linn (1986) Thai J Pharm Sci, 11, pp. 205-211; Bahram, H.A., Lee, A., Bruce, W.H., Daxa, A., Maria, S., Peilin, G., Subhas, C.K., Dietary soybean prevents bone loss in a ovariectomized rat model of Osteoporosis (1996) J Nutr, 126, pp. 161-167",
year = "2010",
language = "English",
volume = "33",
pages = "252--257",
journal = "Chang Gung Medical Journal",
issn = "2072-0939",
publisher = "Chang Gung Medical Journal",
number = "3",

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Anti-osteoporotic activity of the petroleum ether extract of Cissus quadrangularis Linn. in ovariectomized wistar rats. / Potu, Bhagath Kumar; Rao, Muddanna S.; Nampurath, Gopalan Kutty; Chamallamudi, Mallikarjuna Rao; Nayak, Soubhagya Ranjan; Thomas, Huban.

In: Chang Gung Medical Journal, Vol. 33, No. 3, 2010, p. 252-257.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Anti-osteoporotic activity of the petroleum ether extract of Cissus quadrangularis Linn. in ovariectomized wistar rats

AU - Potu, Bhagath Kumar

AU - Rao, Muddanna S.

AU - Nampurath, Gopalan Kutty

AU - Chamallamudi, Mallikarjuna Rao

AU - Nayak, Soubhagya Ranjan

AU - Thomas, Huban

N1 - Cited By :18 Export Date: 10 November 2017 CODEN: CIHCE Correspondence Address: Rao, M. S.; Department of Anatomy, Faculty of Medicine, Kuwait University, P.O. box: 24923, Safat, 13110, Kuwait; email: muddannas@yahoo.com Chemicals/CAS: petroleum ether, 8032-32-4; raloxifene, 82640-04-8, 84449-90-1; Plant Extracts; Raloxifene References: Potu, B.K., Rao, M.S., Swamy, N.V.B., Kutty, G.N., Cissus quadrangularis plant extract enhances the ossification of fetal bones (2007) Pharmacologyonline, 1, pp. 63-70; Rao, M.S., Potu, B.K., Swamy, N.V.B., Kutty, G.N., Cissus Quadrangularis plant extract enhances the development of cortical bone and trabeculae in the fetal femur (2007) Pharmacologyonline, 3, pp. 190-202; Potu, B.K., Rao, M.S., Kutty, G.N., Bhat, M.R.K., Chamallamudi, M.R., Nayak, S.R., Petroleum ether extract of Cissus quadrangularis (Linn) stimulates the growth of fetal bone during intrauterine developmental period: A morphometric analysis (2008) Clinics, 63, pp. 815-820. , (Sao Paulo); Shirwaikar, A., Khan, S., Malini, S., Antiosteoporotic effect of ethanol extract of Cissus quadrangularis Linn. on ovariectomized rat (2003) J Ethnopharmacol, 89, pp. 245-250; Potu, B.K., Bhat, K.M., Rao, M.S., Nampurath, G.K., Chamallamudi, M.R., Nayak, S.R., Petroleum ether extract of Cissus quadrangularis (Linn.) enhances bone marrow mesenchymal stem cell proliferation and facilitates osteoblastogenesis (2009) Clinics, 64, pp. 993-998; Ghosh, M.N., (1984) Fundamentals of Experimental Pharmacology, pp. 153-157. , 2nd ed. Calcutta, India: Scientific Book Agency; Joffe, I., Katz, I., Jacobs, T., Stein, B., Takizawa, M., Liu, C., Berlin, J., Epstein, S., 17 beta-estradiol prevents osteopenia in the oophorectomized rat treated with cyclosporin-A (1992) Endocrinology, 130, pp. 1578-1586; Cheung, J., Mak, Y.T., Papaioannou, S., Evans, B.A.J., Fogelman, I., Hampson, G., Interleukin-6 (IL-6), IL-1, receptor activator of nuclear factor kB ligand (RANKL) and osteoprotegerin production by human osteoblastic cells: Comparison of the effects of 17-beta oestrodiol and raloxifene (2003) J Endocrinol, 177, pp. 423-433; Peng, Z., Tuukkanen, J., Zhang, H., The mechanical strength of bone in different rat models of experimental osteoporosis (1994) Bone, 15, pp. 523-532; Miao, D., Scutt, A., Histochemical localization of alkaline phosphatase activity in decalcified bone and cartilage (2002) J Histochem Cytochem, 50, pp. 333-340; Sen, S.P., Studies on the active constituents of Cissus quadrangularis Wall (1966) Curr Sci, 35, p. 317; Singh, G., Rawat, P., Maurya, R., Constituents of Cissus quadrangularis (2007) Nat Prod Res, 21, pp. 522-528; Bhutani, K.K., Kapoor, R., Atal, C.K., Two unsymmetric tetracyclic triterpenoids from Cissus quadrangularis (1984) Phytochemistry, 23, pp. 407-410; Gupta, M.M., Verma, R.K., Unsymmetric tetracyclic triterpenoids from Cissus quadrangularis (1990) Phytochemistry, 29, pp. 336-337; Mehta, M., Kaur, N., Bhutani, K.K., Determination of marker constituents from Cissus quadiangularis Linn. and their quantitation by HPTLC and HPLC (2001) Phytochem Anal, 12, pp. 91-95; Kausch, A.P., Horner, H.T., A comparison of calcium oxalate crystals isolated from callus cultures and their explant sources (1982) Scan Electron Microsc, 1, pp. 199-211; Gupta, M.M., Verma, R.K., Lipid constituents of Cissus quadrangularis (1991) Phytochemistry, 30, pp. 875-878; Adesanya, S.A., Nia, R., Maartin, M.T., Boukamcha, N., Montagnac, A., Pais, M., Stilbene derivatives from Cissus quadrangularis (1999) J Nat Prod, 62, pp. 1694-1695; Pluemjai, T., Saifah, E., Constituents of Cissus quadrangularis Linn (1986) Thai J Pharm Sci, 11, pp. 205-211; Bahram, H.A., Lee, A., Bruce, W.H., Daxa, A., Maria, S., Peilin, G., Subhas, C.K., Dietary soybean prevents bone loss in a ovariectomized rat model of Osteoporosis (1996) J Nutr, 126, pp. 161-167

PY - 2010

Y1 - 2010

N2 - Background: The plant Cissus quadrangularis (CQ) is used as an osteoprotective agent in Ayurveda, the Indian system of alternative medicine. The present study was done to validate the anti-osteoporotic role of the petroleum ether extract of CQ on ovariectomy-induced osteoporosis in rats. Methods: Female wistar rats were divided into five groups of six rats each; (1) The Normal control (NC) group (n = 6) received no surgery and no treatment, (2). The Sham control (SHAM) group (n = 6) received sham surgery but no treatment, (3) the Ovariectomized (OVX) group (n = 6) received an ovariectomy and normal saline treatment for 90 days, (4) the Ovariectomized + raloxifene (OVX + RAL) group (n = 6) received an ovariectomy and treatment with raloxifene, a known anti-osteoporotic agent for 90 days commencing from 22nd post ovariectomy day and (5) the Ovariectomy + Cissus quadrangularis (OVX + CQ) group (n = 6) received an ovariectomy, and treatment with the petroleum ether extract of CQ 500 mg/kg body weight daily for 90 days commencing from 22nd post ovariectomy day. At the end of the treatment period, rats in all groups were sacrificed and the right femur was used for biomechanical analysis, and the left femur for histomorphometrical analysis. Results: CQ significantly increased the force required to break the femur (p <0.001) and significantly increased the thickness of both cortical (p <0.001) and trabecular bone (p <0.001). This action of CQ was comparable to the action of raloxifene. Conclusions:The petroleum ether extract of CQ stem seems to possess anti-osteoporotic activity in rats.

AB - Background: The plant Cissus quadrangularis (CQ) is used as an osteoprotective agent in Ayurveda, the Indian system of alternative medicine. The present study was done to validate the anti-osteoporotic role of the petroleum ether extract of CQ on ovariectomy-induced osteoporosis in rats. Methods: Female wistar rats were divided into five groups of six rats each; (1) The Normal control (NC) group (n = 6) received no surgery and no treatment, (2). The Sham control (SHAM) group (n = 6) received sham surgery but no treatment, (3) the Ovariectomized (OVX) group (n = 6) received an ovariectomy and normal saline treatment for 90 days, (4) the Ovariectomized + raloxifene (OVX + RAL) group (n = 6) received an ovariectomy and treatment with raloxifene, a known anti-osteoporotic agent for 90 days commencing from 22nd post ovariectomy day and (5) the Ovariectomy + Cissus quadrangularis (OVX + CQ) group (n = 6) received an ovariectomy, and treatment with the petroleum ether extract of CQ 500 mg/kg body weight daily for 90 days commencing from 22nd post ovariectomy day. At the end of the treatment period, rats in all groups were sacrificed and the right femur was used for biomechanical analysis, and the left femur for histomorphometrical analysis. Results: CQ significantly increased the force required to break the femur (p <0.001) and significantly increased the thickness of both cortical (p <0.001) and trabecular bone (p <0.001). This action of CQ was comparable to the action of raloxifene. Conclusions:The petroleum ether extract of CQ stem seems to possess anti-osteoporotic activity in rats.

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