Antibacterial efficacy of essential oil of two different varieties of ocimum (tulsi) on oral microbiota-an invitro study

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Abstract

Introduction: Ocimum (Tulsi) is an Indian sacred plant which has immense medicinal values. There are various varieties of Tulsi grown in India most common being Ocimum sanctum and Ocimum basilicum. Objectives: To test in-vitro the antibacterial efficacy of commercially available essential oil extracts of different varieties of Ocimum (Tulsi) on common oral pathogens. Methodology: Commercially available essential oil of two varieties of Ocimum i.e. Ocimum sanctum and Ocimum basilicum were procured and checked for their antibacterial activity in-vitro. Five common oral pathogens were selected (two aerobic and three anaerobic). The organisms were incubated on respective culture media. Agar well diffusion method was used to check their activity. The oils were tested undiluted form and 1 in 10 dilutions. The activity of oils was compared with chlorhexidine. Results: Both the oils showed antimicrobial activity against all the test strains. The zone of inhibition produced by Ocimum sanctum oil was maximum for Porphyromonas gingivalis (55 mm) followed by Prevotella intermedia (48 mm). The zone produced was much wider than that of chlorhexidine. For Fusobacterium nucleatum, the zone was equivalent to control. For aerobic bacteria, Ocimum sanctum, showed almost equal efficacy as of chlorhexidine but the effect produced by Ocimum basilicum oil was lesser than control. Conclusion: The essential oil of two varieties of Tulsi showed good antimicrobial activity against the common anaerobic and aerobic organisms of the oral cavity. The activity was more pronounced against anaerobes and was found to be better than chlorhexidine. Ocimum sanctum oil produced a wider zone of inhibition as compared to Ocimum basilicum for all the test strains.

Original languageEnglish
Pages (from-to)188-193
Number of pages6
JournalIndian Journal of Public Health Research and Development
Volume10
Issue number6
DOIs
Publication statusPublished - 01-07-2019

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Ocimum
Ocimum basilicum
Microbiota
Volatile Oils
Chlorhexidine
Oils
Prevotella intermedia
Fusobacterium nucleatum
Porphyromonas gingivalis
Aerobic Bacteria
Agar
Culture Media
Mouth
India
Ocimum sanctum
ocimum oil

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health

Cite this

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title = "Antibacterial efficacy of essential oil of two different varieties of ocimum (tulsi) on oral microbiota-an invitro study",
abstract = "Introduction: Ocimum (Tulsi) is an Indian sacred plant which has immense medicinal values. There are various varieties of Tulsi grown in India most common being Ocimum sanctum and Ocimum basilicum. Objectives: To test in-vitro the antibacterial efficacy of commercially available essential oil extracts of different varieties of Ocimum (Tulsi) on common oral pathogens. Methodology: Commercially available essential oil of two varieties of Ocimum i.e. Ocimum sanctum and Ocimum basilicum were procured and checked for their antibacterial activity in-vitro. Five common oral pathogens were selected (two aerobic and three anaerobic). The organisms were incubated on respective culture media. Agar well diffusion method was used to check their activity. The oils were tested undiluted form and 1 in 10 dilutions. The activity of oils was compared with chlorhexidine. Results: Both the oils showed antimicrobial activity against all the test strains. The zone of inhibition produced by Ocimum sanctum oil was maximum for Porphyromonas gingivalis (55 mm) followed by Prevotella intermedia (48 mm). The zone produced was much wider than that of chlorhexidine. For Fusobacterium nucleatum, the zone was equivalent to control. For aerobic bacteria, Ocimum sanctum, showed almost equal efficacy as of chlorhexidine but the effect produced by Ocimum basilicum oil was lesser than control. Conclusion: The essential oil of two varieties of Tulsi showed good antimicrobial activity against the common anaerobic and aerobic organisms of the oral cavity. The activity was more pronounced against anaerobes and was found to be better than chlorhexidine. Ocimum sanctum oil produced a wider zone of inhibition as compared to Ocimum basilicum for all the test strains.",
author = "Kush Kalra and Ramprasad Vasthare and Shenoy, {Padmaja Ananth} and Shashidhar Vishwanath and Singhal, {Deepak Kumar}",
year = "2019",
month = "7",
day = "1",
doi = "10.5958/0976-5506.2019.01264.6",
language = "English",
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pages = "188--193",
journal = "Indian Journal of Public Health Research and Development",
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T1 - Antibacterial efficacy of essential oil of two different varieties of ocimum (tulsi) on oral microbiota-an invitro study

AU - Kalra, Kush

AU - Vasthare, Ramprasad

AU - Shenoy, Padmaja Ananth

AU - Vishwanath, Shashidhar

AU - Singhal, Deepak Kumar

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Introduction: Ocimum (Tulsi) is an Indian sacred plant which has immense medicinal values. There are various varieties of Tulsi grown in India most common being Ocimum sanctum and Ocimum basilicum. Objectives: To test in-vitro the antibacterial efficacy of commercially available essential oil extracts of different varieties of Ocimum (Tulsi) on common oral pathogens. Methodology: Commercially available essential oil of two varieties of Ocimum i.e. Ocimum sanctum and Ocimum basilicum were procured and checked for their antibacterial activity in-vitro. Five common oral pathogens were selected (two aerobic and three anaerobic). The organisms were incubated on respective culture media. Agar well diffusion method was used to check their activity. The oils were tested undiluted form and 1 in 10 dilutions. The activity of oils was compared with chlorhexidine. Results: Both the oils showed antimicrobial activity against all the test strains. The zone of inhibition produced by Ocimum sanctum oil was maximum for Porphyromonas gingivalis (55 mm) followed by Prevotella intermedia (48 mm). The zone produced was much wider than that of chlorhexidine. For Fusobacterium nucleatum, the zone was equivalent to control. For aerobic bacteria, Ocimum sanctum, showed almost equal efficacy as of chlorhexidine but the effect produced by Ocimum basilicum oil was lesser than control. Conclusion: The essential oil of two varieties of Tulsi showed good antimicrobial activity against the common anaerobic and aerobic organisms of the oral cavity. The activity was more pronounced against anaerobes and was found to be better than chlorhexidine. Ocimum sanctum oil produced a wider zone of inhibition as compared to Ocimum basilicum for all the test strains.

AB - Introduction: Ocimum (Tulsi) is an Indian sacred plant which has immense medicinal values. There are various varieties of Tulsi grown in India most common being Ocimum sanctum and Ocimum basilicum. Objectives: To test in-vitro the antibacterial efficacy of commercially available essential oil extracts of different varieties of Ocimum (Tulsi) on common oral pathogens. Methodology: Commercially available essential oil of two varieties of Ocimum i.e. Ocimum sanctum and Ocimum basilicum were procured and checked for their antibacterial activity in-vitro. Five common oral pathogens were selected (two aerobic and three anaerobic). The organisms were incubated on respective culture media. Agar well diffusion method was used to check their activity. The oils were tested undiluted form and 1 in 10 dilutions. The activity of oils was compared with chlorhexidine. Results: Both the oils showed antimicrobial activity against all the test strains. The zone of inhibition produced by Ocimum sanctum oil was maximum for Porphyromonas gingivalis (55 mm) followed by Prevotella intermedia (48 mm). The zone produced was much wider than that of chlorhexidine. For Fusobacterium nucleatum, the zone was equivalent to control. For aerobic bacteria, Ocimum sanctum, showed almost equal efficacy as of chlorhexidine but the effect produced by Ocimum basilicum oil was lesser than control. Conclusion: The essential oil of two varieties of Tulsi showed good antimicrobial activity against the common anaerobic and aerobic organisms of the oral cavity. The activity was more pronounced against anaerobes and was found to be better than chlorhexidine. Ocimum sanctum oil produced a wider zone of inhibition as compared to Ocimum basilicum for all the test strains.

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